Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,432,266	G→A	P67S (CCT→TCT)	smg ←	DUF494 domain‑containing protein Smg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,432,266	0	G	A	100.0%	286.5 / NA	80	P67S (CCT→TCT)	smg	DUF494 domain‑containing protein Smg
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/33);  total (47/33)

CGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATC  >  NC_000913/3432129‑3432401                                                                                                                                          |                                                                                                                                        cgcgAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAAt                                                                                                                                       >  2:370487/1‑140 (MQ=255) cgcgAGCACTCGCCCTATCACCATTTCACGAGTTTCTAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAAt                                                                                                                                       >  2:454883/1‑140 (MQ=255)                tATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAATCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCa                                                                                                                        <  2:1115128/140‑1 (MQ=255)                tATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCa                                                                                                                        <  2:1129550/140‑1 (MQ=255)                tATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCa                                                                                                                        >  1:1115128/1‑140 (MQ=255)                    aCCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCgg                                                                                                                    <  1:98455/140‑1 (MQ=255)                     ccATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGt                                                                                                                   <  1:384260/140‑1 (MQ=255)                      cATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCa                                                                                                                        >  1:102590/1‑134 (MQ=255)                      cATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCa                                                                                                                        <  2:102590/134‑1 (MQ=255)                        tttCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGtt                                                                                                                  >  1:277103/1‑138 (MQ=255)                        tttCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGtt                                                                                                                  <  2:277103/138‑1 (MQ=255)                        tttCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCt                                                                                                                >  2:1091155/1‑140 (MQ=255)                        tttCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCt                                                                                                                >  2:914541/1‑140 (MQ=255)                        tttCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCt                                                                                                                >  2:1216189/1‑140 (MQ=255)                        tttCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCt                                                                                                                >  2:622858/1‑140 (MQ=255)                        tttCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATACGAGGCCAGTTGCATCGGTTCt                                                                                                                >  1:905698/1‑140 (MQ=255)                             tgAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCaa                                                                                                           <  2:434834/139‑1 (MQ=255)                             cGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCaa                                                                                                           <  1:731674/140‑1 (MQ=255)                             cGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCaa                                                                                                           <  1:601302/140‑1 (MQ=255)                             cGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCaa                                                                                                           <  1:558421/140‑1 (MQ=255)                                gTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGGCAGTTGCATCGGTTCTGCCAAccc                                                                                                        <  1:317830/140‑1 (MQ=255)                                   tCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTc                                                                                                     >  1:377414/1‑140 (MQ=255)                                          tGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATaa                                                                                              <  2:377414/140‑1 (MQ=255)                                            aGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATc                                                                                            >  1:824872/1‑140 (MQ=255)                                            aGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATc                                                                                            >  1:849816/1‑140 (MQ=255)                                            aGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATc                                                                                            >  2:1006837/1‑140 (MQ=255)                                            aGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATc                                                                                            >  2:1096571/1‑140 (MQ=255)                                            aGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATc                                                                                            >  1:313609/1‑140 (MQ=255)                                                  tGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAg                                                                                      >  2:162979/1‑140 (MQ=255)                                                      tCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGtttt                                                                                  >  1:1158539/1‑140 (MQ=255)                                                       cTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGttttt                                                                                 <  1:156487/140‑1 (MQ=255)                                                         gCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACTCTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAACAAGTTTTTcc                                                                               <  1:1091155/140‑1 (MQ=255)                                                         gCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTcc                                                                               <  1:137480/140‑1 (MQ=255)                                                           tCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAg                                                                             >  2:427171/1‑140 (MQ=255)                                                              aGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCa                                                                          >  2:110821/1‑140 (MQ=255)                                                               ggAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCAt                                                                         >  1:682169/1‑140 (MQ=255)                                                               ggAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCAt                                                                         >  1:75125/1‑140 (MQ=255)                                                                   aaGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCa                                                                     <  1:1096571/140‑1 (MQ=255)                                                                    aGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAg                                                                    >  1:580279/1‑140 (MQ=255)                                                                    aGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAg                                                                    >  2:711230/1‑140 (MQ=255)                                                                     gCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAgg                                                                   <  1:427171/140‑1 (MQ=255)                                                                      cAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAggg                                                                  <  1:126478/140‑1 (MQ=255)                                                                       aGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGc                                                                 <  1:167486/140‑1 (MQ=255)                                                                       aGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGc                                                                 <  1:291155/140‑1 (MQ=255)                                                                        gAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCa                                                                <  2:74300/140‑1 (MQ=255)                                                                        gAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCa                                                                <  2:736413/140‑1 (MQ=255)                                                                        gAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCa                                                                <  1:454883/140‑1 (MQ=255)                                                                              catggCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAg                                                          <  2:785516/137‑1 (MQ=255)                                                                                     gCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATAtctt                                                   >  1:1022753/1‑140 (MQ=255)                                                                                      cTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCa                                                                >  2:95724/1‑126 (MQ=255)                                                                                      cTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCa                                                                <  1:95724/126‑1 (MQ=255)                                                                                      cTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATAtcttc                                                  >  1:217461/1‑140 (MQ=255)                                                                                       tGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATAtcttct                                                 <  2:580279/140‑1 (MQ=255)                                                                                           aTCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGATAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCt                                             >  1:1118632/1‑140 (MQ=255)                                                                                                    ttCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTg                                    >  1:459470/1‑140 (MQ=255)                                                                                                    ttCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTg                                    >  1:335693/1‑140 (MQ=255)                                                                                                      cacaCTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCg                                  <  2:335693/140‑1 (MQ=255)                                                                                                       acacTCTTCCGGTGTATAAATACGCATGGAGATAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGt                                 <  2:1118632/140‑1 (MQ=255)                                                                                                       acacTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGt                                 <  2:217461/140‑1 (MQ=255)                                                                                                        cacTCTTCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTc                                >  1:870249/1‑140 (MQ=255)                                                                                                             ttCCGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTaa                           >  1:1243881/1‑140 (MQ=255)                                                                                                               ccGGTGTATAAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCGTTGTAGATATCTTCTCGCTCAAACCCTGCGTCGGTAAGa                         <  2:75125/140‑1 (MQ=255)                                                                                                                     tataAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGt                   >  1:198303/1‑140 (MQ=255)                                                                                                                      ataAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGtt                  <  1:711230/140‑1 (MQ=255)                                                                                                                       taAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCg                                  <  1:381166/123‑1 (MQ=255)                                                                                                                       taAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGt                                 <  1:836975/124‑1 (MQ=255)                                                                                                                       taAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGt                                 >  2:836975/1‑124 (MQ=255)                                                                                                                       taAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTc                 >  2:1110942/1‑140 (MQ=255)                                                                                                                       taAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTc                 >  1:1084555/1‑140 (MQ=255)                                                                                                                       taAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTc                 >  2:115875/1‑140 (MQ=255)                                                                                                                       taAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTc                 >  1:899617/1‑140 (MQ=255)                                                                                                                       taAATACGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTACg                                  >  2:381166/1‑123 (MQ=255)                                                                                                                             cGCATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGttt           >  2:460336/1‑140 (MQ=255)                                                                                                                               cATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGt         >  2:44943/1‑140 (MQ=255)                                                                                                                               cATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGt         >  1:510789/1‑140 (MQ=255)                                                                                                                               cATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGt         >  1:536499/1‑140 (MQ=255)                                                                                                                               cATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGt         >  1:712575/1‑140 (MQ=255)                                                                                                                               cATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGt         >  2:218614/1‑140 (MQ=255)                                                                                                                               cATGGAGAGAGAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGt         >  2:817137/1‑140 (MQ=255)                                                                                                                                     gagagaATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGAt   <  2:359576/140‑1 (MQ=255)                                                                                                                                     gagagaATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGAt   <  2:1022753/140‑1 (MQ=255)                                                                                                                                      agagAATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATc  >  1:547218/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAGTTTTTCCAGCCATAGCAGGGCATTGTAGATATCTTCTCGCTCAAAACCTGCGTCGGTAAGATCCTGTTCAAGTTTGTCTTGATC  >  NC_000913/3432129‑3432401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑