Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,473,215	T→C	E100E (GAA→GAG)	fusA ←	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,473,215	0	T	C	100.0%	307.5 / NA	82	E100E (GAA→GAG)	fusA	elongation factor G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/42);  total (40/42)

TTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTTCCTGCTCCT  >  NC_000913/3473090‑3473351                                                                                                                              |                                                                                                                                         ttAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATtgt                                                                                                                            <  1:443898/140‑1 (MQ=255)  tAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATtgtg                                                                                                                           >  2:445486/1‑140 (MQ=255)    acgaacgCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACtt                                                                                                                                   >  1:1003650/1‑130 (MQ=255)    acgaacgCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACtt                                                                                                                                   <  2:1003650/130‑1 (MQ=255)       aacgCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACTATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTc                                                                                                                      >  2:880460/1‑140 (MQ=255)         cgCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCaa                                                                                                                    >  1:850712/1‑140 (MQ=255)           cAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAAc                                                                                                                   >  1:599809/1‑139 (MQ=255)           cAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAAc                                                                                                                   >  2:423528/1‑139 (MQ=255)           cAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAAc                                                                                                                   <  1:423528/139‑1 (MQ=255)           cAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAAc                                                                                                                   <  2:599809/139‑1 (MQ=255)               gcgcGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGcc                                                                                                              >  1:189395/1‑140 (MQ=255)                  cgGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGTGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCg                                                                                                           <  2:745304/140‑1 (MQ=255)                  cgGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCg                                                                                                           <  1:24871/140‑1 (MQ=255)                  cgGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCg                                                                                                           <  1:445486/140‑1 (MQ=255)                      aCTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGgt                                                                                                       <  1:592373/140‑1 (MQ=255)                        tttATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAGACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGgtgt                                                                                                     <  2:577467/140‑1 (MQ=255)                        tttATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGgtgt                                                                                                     >  1:996209/1‑140 (MQ=255)                              tttgtttgCCTGACGCCATACGGTTTCAGACTGCAGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCgatga                                                                                               <  1:1106985/140‑1 (MQ=255)                               ttgtttgCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCgatgat                                                                                              >  1:574387/1‑140 (MQ=255)                               ttgtttgCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCgatgat                                                                                              >  2:258452/1‑140 (MQ=255)                                 gtttgCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGt                                                                                            >  2:545153/1‑140 (MQ=255)                                 gtttgCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGt                                                                                            >  1:575841/1‑140 (MQ=255)                                 gtttgCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGT‑AACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCgggg                                                                                                        <  1:1119003/127‑1 (MQ=255)                                 gtttgCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGT‑AACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCgggg                                                                                                        >  2:1119003/1‑127 (MQ=255)                                  tttgCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGtt                                                                                           >  1:1127616/1‑140 (MQ=255)                                  tttgCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGtt                                                                                           >  2:961976/1‑140 (MQ=255)                                  tttgCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGtt                                                                                           >  1:1000273/1‑140 (MQ=255)                                    tgCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTga                                                                                         <  1:858825/140‑1 (MQ=255)                                      ccTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTgatg                                                                                       <  1:197578/140‑1 (MQ=255)                                          aCGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTgatgcgat                                                                                   <  2:36152/140‑1 (MQ=255)                                          aCGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTgatgcgat                                                                                   <  1:52511/140‑1 (MQ=255)                                          aCGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTgatgcgat                                                                                   <  2:189395/140‑1 (MQ=255)                                          aCGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTgatgcgat                                                                                   >  2:824347/1‑140 (MQ=255)                                          aCGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTgatgcgat                                                                                   <  1:961976/140‑1 (MQ=255)                                             ccATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGatgcgatgcg                                                                                <  1:258452/140‑1 (MQ=255)                                                tACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTgatgcgat                                                                                   <  2:957863/134‑1 (MQ=255)                                                tACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTgatgcgat                                                                                   >  1:957863/1‑134 (MQ=255)                                                tACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCt                                                                             >  1:667804/1‑140 (MQ=255)                                                   ggTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCAt                                                                          <  2:574387/140‑1 (MQ=255)                                                     tttCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATAc                                                                        >  1:263408/1‑140 (MQ=255)                                                     tttCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATAc                                                                        >  1:552937/1‑140 (MQ=255)                                                     tttCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATAc                                                                        >  2:175690/1‑140 (MQ=255)                                                     tttCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATAc                                                                        >  2:1099589/1‑140 (MQ=255)                                                      ttCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCAt                                                                          >  1:1094693/1‑137 (MQ=255)                                                      ttCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCAt                                                                          <  2:1094693/137‑1 (MQ=255)                                                       tCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTg                                                                      >  2:555957/1‑140 (MQ=255)                                                        cAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGc                                                                     <  2:263408/140‑1 (MQ=255)                                                             tGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGc                                                                <  1:824347/140‑1 (MQ=255)                                                             tGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGc                                                                <  1:545153/140‑1 (MQ=255)                                                                    gAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATacca                                                         <  1:461803/140‑1 (MQ=255)                                                                             caACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAAt                                                <  2:1000273/140‑1 (MQ=255)                                                                               aCTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGc                                              >  1:750869/1‑140 (MQ=255)                                                                                    gcAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCagtag                                         >  2:973943/1‑140 (MQ=255)                                                                                           aCCATTACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGc                                  >  1:465058/1‑140 (MQ=255)                                                                                                tACCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAg                             >  2:956478/1‑140 (MQ=255)                                                                                                 aCCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGt                            <  1:909354/140‑1 (MQ=255)                                                                                                 aCCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGt                            <  2:1207740/140‑1 (MQ=255)                                                                                                 aCCGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGt                            <  1:5890/140‑1 (MQ=255)                                                                                                  ccGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTg                           >  2:111332/1‑140 (MQ=255)                                                                                                  ccGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTg                           >  2:49475/1‑140 (MQ=255)                                                                                                   cGCACCATCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGa                          <  1:1099589/140‑1 (MQ=255)                                                                                                         aTCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGc                                  >  1:960342/1‑126 (MQ=255)                                                                                                         aTCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGc                                  <  2:960342/126‑1 (MQ=255)                                                                                                         aTCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTaa                    >  2:596044/1‑140 (MQ=255)                                                                                                          tCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCg                                 <  1:631510/126‑1 (MQ=255)                                                                                                          tCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCg                                 >  2:631510/1‑126 (MQ=255)                                                                                                          tCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGt                            >  1:524870/1‑131 (MQ=255)                                                                                                          tCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGt                            <  2:524870/131‑1 (MQ=255)                                                                                                          tCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAAt                   >  1:642122/1‑140 (MQ=255)                                                                                                          tCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAAt                   >  2:258412/1‑140 (MQ=255)                                                                                                          tCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAAt                   >  2:850520/1‑140 (MQ=255)                                                                                                          tCGAGAACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAAt                   >  2:1137448/1‑140 (MQ=255)                                                                                                            gagaACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATAc                 <  1:850520/140‑1 (MQ=255)                                                                                                              gaACACGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCa               <  1:111332/140‑1 (MQ=255)                                                                                                                   cGCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTtc          <  1:49475/140‑1 (MQ=255)                                                                                                                    gCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTtcc         <  1:596044/140‑1 (MQ=255)                                                                                                                    gCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTtcc         <  2:552937/140‑1 (MQ=255)                                                                                                                    gCATGGAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTtcc         >  2:1060837/1‑140 (MQ=255)                                                                                                                        ggAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTtcctgct     <  1:478065/140‑1 (MQ=255)                                                                                                                         gAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTtcctgctc    <  1:956478/140‑1 (MQ=255)                                                                                                                         gAACGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTtcctgctc    <  1:1060837/140‑1 (MQ=255)                                                                                                                           aCGCTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTtcctgctcct  <  2:642122/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                         TTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCGGCTCATACTGCTTAGCCATACCAGACCAGAATGCAGTAGTCGCAGCGGAAGTGATGGTAATACCACGTTCCTGCTCCT  >  NC_000913/3473090‑3473351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑