Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,487,815	A→G	D342G (GAC→GGC)	yhfK →	putative transporter YhfK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,487,815	0	A	G	100.0%	246.2 / NA	66	D342G (GAC→GGC)	yhfK	putative transporter YhfK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/34);  total (32/34)

CGATGGAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGACTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGCCTGATGGGCA  >  NC_000913/3487680‑3487946                                                                                                                                        |                                                                                                                                    cGATGGAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTa                                                                                                                                 <  2:461790/140‑1 (MQ=255) cGATGGAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTa                                                                                                                                 <  1:299250/140‑1 (MQ=255)   aTGGAAAGGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACt                                                                                                                               <  1:752717/140‑1 (MQ=255)   aTGGAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACt                                                                                                                               <  2:551990/140‑1 (MQ=255)      gAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGc                                                                                                                            >  1:115259/1‑140 (MQ=255)        aaaGCCAATTGGCGCACTGGAAAAAAACGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACTCAAAAACCGCTCTACGCCCGTGGCTTACTGGGCg                                                                                                                          <  2:1133778/140‑1 (MQ=255)           gCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATa                                                                                                                       <  1:806946/140‑1 (MQ=255)               aTTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACa                                                                                                                   <  1:479451/140‑1 (MQ=255)               aTTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACa                                                                                                                   <  2:731463/140‑1 (MQ=255)               aTTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACa                                                                                                                   >  2:581387/1‑140 (MQ=255)                ttGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAg                                                                                                                  <  2:435355/140‑1 (MQ=255)                ttGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAg                                                                                                                  <  1:1210011/140‑1 (MQ=255)                ttGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAg                                                                                                                  >  2:423686/1‑140 (MQ=255)                      cACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGa                                                                                                            >  2:516030/1‑140 (MQ=255)                         tGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATg                                                                                                         >  1:162897/1‑140 (MQ=255)                           gAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGcc                                                                                                       >  2:1260617/1‑140 (MQ=255)                                     cccgccAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAgcggcg                                                                                                             <  1:581939/124‑1 (MQ=255)                                     cccgccAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAgcggcg                                                                                                             >  2:581939/1‑124 (MQ=255)                                     cccgccAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCg                                                                                             >  2:732795/1‑140 (MQ=255)                                                    aTAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTAt                                                                              <  2:349840/140‑1 (MQ=255)                                                      aaTCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCt                                                                            <  1:732795/140‑1 (MQ=255)                                                        tCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTgc                                                                          >  1:83775/1‑139 (MQ=255)                                                        tCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGAGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGCTATCTGt                                                                          >  1:873746/1‑140 (MQ=255)                                                             ttGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTa                                                                     >  1:311127/1‑140 (MQ=255)                                                                  cAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGtc                                                                >  1:54124/1‑140 (MQ=255)                                                                    aTTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGtctc                                                              <  2:311127/140‑1 (MQ=255)                                                                          cTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGgcgc                                                        >  2:1191648/1‑140 (MQ=255)                                                                           tACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCt                                                       >  2:1136807/1‑140 (MQ=255)                                                                              tGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACg                                                    <  1:516030/140‑1 (MQ=255)                                                                              tGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACg                                                    <  1:364399/140‑1 (MQ=255)                                                                               ggCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGc                                                   >  1:1016862/1‑140 (MQ=255)                                                                                gCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACTGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGTCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACg                                                    <  1:750022/138‑1 (MQ=255)                                                                                gCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACTGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGTCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACg                                                    >  2:750022/1‑138 (MQ=255)                                                                                gCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCa                                                  >  2:642433/1‑140 (MQ=255)                                                                                 cATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCaa                                                 <  1:696167/140‑1 (MQ=255)                                                                                   tttCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATg                                               >  1:1091650/1‑140 (MQ=255)                                                                                    ttCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGc                                              >  2:730921/1‑140 (MQ=255)                                                                                    ttCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGc                                              >  1:898905/1‑140 (MQ=255)                                                                                          cGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGgacg                                        >  1:622078/1‑140 (MQ=255)                                                                                             aTCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACt                                     <  1:581387/140‑1 (MQ=255)                                                                                              tCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTc                                    >  1:177389/1‑140 (MQ=255)                                                                                               cGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCa                                   <  1:1260617/140‑1 (MQ=255)                                                                                               cGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCa                                   <  1:1191648/140‑1 (MQ=255)                                                                                               cGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCa                                   <  2:779044/140‑1 (MQ=255)                                                                                                  ccGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAgtg                                <  1:423686/140‑1 (MQ=255)                                                                                                   cgcgTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAgtgt                               <  1:1136807/140‑1 (MQ=255)                                                                                                       tgctgcGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATg                           >  1:105552/1‑140 (MQ=255)                                                                                                         ctgcGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGtt                         >  2:1045831/1‑140 (MQ=255)                                                                                                          tgcGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTa                        >  2:319345/1‑140 (MQ=255)                                                                                                          tgcGCACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTa                        <  1:642433/140‑1 (MQ=255)                                                                                                            cgcACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAg                      >  2:143054/1‑140 (MQ=255)                                                                                                            cgcACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAg                      <  1:105846/140‑1 (MQ=255)                                                                                                            cgcACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAg                      <  1:477622/140‑1 (MQ=255)                                                                                                              cACCCAAAAACCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCg                    >  2:195603/1‑140 (MQ=255)                                                                                                                    aaaaCCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCa              <  2:115259/140‑1 (MQ=255)                                                                                                                    aaaaCCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCa              >  1:858820/1‑140 (MQ=255)                                                                                                                      aaCCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGc            <  2:162897/140‑1 (MQ=255)                                                                                                                      aaCCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGc            <  2:54124/140‑1 (MQ=255)                                                                                                                      aaCCGCTCTATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGc            <  1:730921/140‑1 (MQ=255)                                                                                                                           ctctATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGc            <  2:997702/135‑1 (MQ=255)                                                                                                                           ctctATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGc            >  1:997702/1‑135 (MQ=255)                                                                                                                           ctctATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGCCTGAt       >  1:8013/1‑140 (MQ=255)                                                                                                                           ctctATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGCCTGAt       >  2:1249075/1‑140 (MQ=255)                                                                                                                              tATGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGCCTGATggg    <  1:505592/140‑1 (MQ=255)                                                                                                                                tGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGCCTGATGGGCa  >  1:883105/1‑140 (MQ=255)                                                                                                                                tGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGCCTGATGGGCa  <  1:195603/140‑1 (MQ=255)                                                                                                                                tGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGCCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGCCTGATGGGCa  <  2:83775/140‑1 (MQ=255)                                                                                                                                tGCCCGTGGCTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCAGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGCCTGATGGGCa  >  2:501117/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    CGATGGAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGACTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTATCTGTCACTAAAGTCTCCGGCGCTACGCAATGCCGGACGACTCAGTGTGATGTTAAGCGTTGCCAGCCTGATGGGCA  >  NC_000913/3487680‑3487946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑