Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,657,230	T→C	V79A (GTA→GCA)	hdeD →	acid‑resistance membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,657,230	0	T	C	100.0%	295.7 / NA	79	V79A (GTA→GCA)	hdeD	acid‑resistance membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/35);  total (44/35)

GTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGCCTGATG  >  NC_000913/3657094‑3657366                                                                                                                                         |                                                                                                                                         gtcgGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGcatt                                                                                                                                       <  1:1048805/140‑1 (MQ=255)  tcgGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGcatta                                                                                                                                      >  2:553358/1‑140 (MQ=255)  tcgGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGcatta                                                                                                                                      >  1:214396/1‑140 (MQ=255)  tcgGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGcatta                                                                                                                                      >  1:547084/1‑140 (MQ=255)     ggTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATcc                                                                                                                                   >  2:611128/1‑140 (MQ=255)       ttGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCgg                                                                                                                                 >  1:1030580/1‑140 (MQ=255)        tGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGt                                                                                                                                <  2:755459/140‑1 (MQ=255)           tgtgTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTc                                                                                                                             <  2:1109620/140‑1 (MQ=255)               tATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCtcg                                                                                                                         <  2:685752/140‑1 (MQ=255)                  cAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCtcgtcg                                                                                                                      <  2:1132579/140‑1 (MQ=255)                    gTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCa                                                                                                                    >  1:881532/1‑140 (MQ=255)                    gTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCa                                                                                                                    <  1:611128/140‑1 (MQ=255)                      ttCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTCGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCTCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGt                                                                                                                  <  2:881532/140‑1 (MQ=255)                      ttCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGt                                                                                                                  <  2:1030580/140‑1 (MQ=255)                      ttCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGt                                                                                                                  <  2:805463/140‑1 (MQ=255)                        cccGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCg                                                                                                                <  1:294567/140‑1 (MQ=255)                         ccGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGc                                                                                                               <  1:39889/140‑1 (MQ=255)                         ccGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGc                                                                                                               <  1:716853/140‑1 (MQ=255)                         ccGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGc                                                                                                               <  2:775336/140‑1 (MQ=255)                               gTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGt                                                                                                                  <  1:392012/131‑1 (MQ=255)                               gTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGt                                                                                                                  >  2:392012/1‑131 (MQ=255)                               gTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCtattt                                                                                                         <  2:799663/140‑1 (MQ=255)                                          tatTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCt                                                                                              <  2:192307/140‑1 (MQ=255)                                          tatTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCt                                                                                              <  1:1143457/140‑1 (MQ=255)                                          tatTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCt                                                                                              <  2:1227640/140‑1 (MQ=255)                                           atTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTa                                                                                             >  2:961972/1‑140 (MQ=255)                                               tAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATttc                                                                                         <  1:553358/140‑1 (MQ=255)                                                      agtagtGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGACGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATcc                                                                                  >  1:682637/1‑140 (MQ=255)                                                       gtagtGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCcg                                                                                 <  1:786739/140‑1 (MQ=255)                                                        tagtGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCcgc                                                                                >  1:843322/1‑140 (MQ=255)                                                             ggTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCAt                                                                                    >  1:78239/1‑131 (MQ=255)                                                             ggTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCAt                                                                                    <  2:78239/131‑1 (MQ=255)                                                                gCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGa                                                                        >  1:7315/1‑140 (MQ=255)                                                                gCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGa                                                                        >  2:433947/1‑140 (MQ=255)                                                                  attattAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGc                                                                      >  1:588412/1‑140 (MQ=255)                                                                    tattaATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCcg                                                                                 >  2:965987/1‑127 (MQ=255)                                                                    tattaATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCcg                                                                                 <  1:965987/127‑1 (MQ=255)                                                                     attaATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTgg                                                                   >  1:100743/1‑140 (MQ=255)                                                                     attaATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTgg                                                                   >  2:599517/1‑140 (MQ=255)                                                                      ttaATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCcgcg                                                                               <  1:67712/127‑1 (MQ=255)                                                                      ttaATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCcgcg                                                                               >  2:67712/1‑127 (MQ=255)                                                                       taATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGc                                                                 >  1:768091/1‑140 (MQ=255)                                                                              cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGTCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:1117593/1‑140 (MQ=255)                                                                              cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  2:807507/1‑140 (MQ=255)                                                                              cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  2:999917/1‑140 (MQ=255)                                                                              cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  2:662792/1‑140 (MQ=255)                                                                              cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:573532/1‑140 (MQ=255)                                                                              cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:330263/1‑140 (MQ=255)                                                                              cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  2:9076/1‑140 (MQ=255)                                                                              cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  2:335553/1‑140 (MQ=255)                                                                              cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:1147837/1‑140 (MQ=255)                                                                              cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:696861/1‑140 (MQ=255)                                                                              cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:765112/1‑140 (MQ=255)                                                                              cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:949062/1‑140 (MQ=255)                                                                              cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGACTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:225568/1‑140 (MQ=255)                                                                                cGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGcc                                                        >  1:1164537/1‑140 (MQ=255)                                                                                      ttGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTgg                                                                   <  2:268815/123‑1 (MQ=255)                                                                                      ttGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTgg                                                                   >  1:268815/1‑123 (MQ=255)                                                                                      ttGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCg                                                  >  2:901344/1‑140 (MQ=255)                                                                                      ttGCGCTTATTGTCGGGTTATTCAGCAACCGCAGGCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCg                                                  >  1:200099/1‑140 (MQ=255)                                                                                        gcgcTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGc                                                >  2:1036341/1‑140 (MQ=255)                                                                                         cgcTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCa                                                       >  1:406582/1‑132 (MQ=255)                                                                                         cgcTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCa                                                       <  2:406582/132‑1 (MQ=255)                                                                                         cgcTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCa                                               >  2:471517/1‑140 (MQ=255)                                                                                           cTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCAtt                                             >  2:1193877/1‑140 (MQ=255)                                                                                              aTTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTAt                                          >  1:465381/1‑140 (MQ=255)                                                                                                tGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTg                                        <  1:433947/140‑1 (MQ=255)                                                                                                   cGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCg                                     >  1:930668/1‑140 (MQ=255)                                                                                                     ggTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGt                                   <  2:843322/140‑1 (MQ=255)                                                                                                         aTTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGt                               >  2:145878/1‑140 (MQ=255)                                                                                                             aGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCtg                           <  1:662792/140‑1 (MQ=255)                                                                                                               cAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGt                                   <  2:803438/130‑1 (MQ=255)                                                                                                               cAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGt                                   >  1:803438/1‑130 (MQ=255)                                                                                                                      aGTCATAATTTCTGGCCGGCATTATCCGGTTTTCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAgg                  <  2:353164/140‑1 (MQ=255)                                                                                                                           tAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAAGGGTTa             <  2:588412/140‑1 (MQ=255)                                                                                                                             aTTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTAtt           <  2:1096018/140‑1 (MQ=255)                                                                                                                             aTTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTAtt           <  2:100743/140‑1 (MQ=255)                                                                                                                              tttCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTc          <  2:465381/140‑1 (MQ=255)                                                                                                                                tCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGc        <  2:765112/140‑1 (MQ=255)                                                                                                                                tCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGc        >  2:266643/1‑140 (MQ=255)                                                                                                                                  tGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGCCt      >  2:797270/1‑140 (MQ=255)                                                                                                                                  tGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGCCt      >  1:1253266/1‑140 (MQ=255)                                                                                                                                    gCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGACTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGCCtga    <  2:225568/140‑1 (MQ=255)                                                                                                                                      cGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGCCtgatg  <  1:469795/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGCCTGATG  >  NC_000913/3657094‑3657366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑