Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,923,928	A→G	L569P (CTG→CCG)	mnmG ←	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,923,928	0	A	G	100.0%	179.7 / NA	49	L569P (CTG→CCG)	mnmG	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/27);  total (22/27)

TGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGT  >  NC_000913/3923794‑3924064                                                                                                                                       |                                                                                                                                         tGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCggg                                                                                                                                        >  1:718825/1‑137 (MQ=255) tGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGgtg                                                                                                                                     >  2:951464/1‑140 (MQ=255)    agaTGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCTATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTtc                                                                                                                                  >  2:56891/1‑140 (MQ=255)        ggCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGt                                                                                                                              <  1:951464/140‑1 (MQ=255)         gCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGtt                                                                                                                             >  2:670588/1‑140 (MQ=255)          ccGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCTATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTa                                                                                                                            <  1:56891/140‑1 (MQ=255)          ccGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTa                                                                                                                            <  1:389021/140‑1 (MQ=255)          ccGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTa                                                                                                                            <  1:464026/140‑1 (MQ=255)           cGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTAc                                                                                                                           >  2:741572/1‑140 (MQ=255)                gCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAgc                                                                                                                      >  2:914191/1‑140 (MQ=255)                   tGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAgctgc                                                                                                                   >  2:145048/1‑140 (MQ=255)                   tGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAgctgc                                                                                                                   >  1:939280/1‑140 (MQ=255)                     aCGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCAATTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCtt                                                                                                                 >  2:1191403/1‑140 (MQ=255)                          aGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGa                                                                                                            <  1:1148405/140‑1 (MQ=255)                          aGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGa                                                                                                            >  2:724885/1‑140 (MQ=255)                           gAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGAt                                                                                                           <  2:528909/140‑1 (MQ=255)                             aaTACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGAtct                                                                                                         <  1:724885/140‑1 (MQ=255)                             aaTACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGAtct                                                                                                         <  1:914191/140‑1 (MQ=255)                                  gcgAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCt                                                                                                    >  2:455732/1‑140 (MQ=255)                                    gAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTg                                                                                                  <  1:884424/140‑1 (MQ=255)                                       gCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTg                                                                                               <  1:670588/140‑1 (MQ=255)                                           ggCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGgcgc                                                                                           <  2:611587/140‑1 (MQ=255)                                            gCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGAtct                                                                                                         <  1:21781/125‑1 (MQ=255)                                            gCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGAtct                                                                                                         >  2:21781/1‑125 (MQ=255)                                            gCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGgcgcg                                                                                          >  2:756276/1‑140 (MQ=255)                                            gCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGgcgcg                                                                                          <  2:390986/140‑1 (MQ=255)                                            gCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGgcgcg                                                                                          <  1:741572/140‑1 (MQ=255)                                                 tAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGata                                                                                     <  1:356474/140‑1 (MQ=255)                                                  agagGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGatat                                                                                    <  1:1191403/140‑1 (MQ=255)                                                          gTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCg                                                                            >  1:1208006/1‑140 (MQ=255)                                                           tttGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGt                                                                           >  1:708917/1‑140 (MQ=255)                                                                    gTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCt                                                                  <  1:360955/140‑1 (MQ=255)                                                                    gTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCt                                                                  <  1:756276/140‑1 (MQ=255)                                                                    gTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCt                                                                  <  1:264733/140‑1 (MQ=255)                                                                    gTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCt                                                                  <  2:667289/140‑1 (MQ=255)                                                                         gTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTGCGCAGCTGCTTTTCGATCTCATCTTGCTGGAGCGCGATATAACCTTCGTATTTAACCTGAAtc                                                             <  2:512554/140‑1 (MQ=255)                                                                         gTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAAtc                                                             <  1:886379/140‑1 (MQ=255)                                                                            tGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCa                                                          <  1:363120/140‑1 (MQ=255)                                                                            tGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCa                                                          <  1:455732/140‑1 (MQ=255)                                                                             ggCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCaa                                                         >  2:1209034/1‑140 (MQ=255)                                                                                              aaaGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGt                                        >  1:1173899/1‑140 (MQ=255)                                                                                                aGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTc                                      >  2:971278/1‑140 (MQ=255)                                                                                                  aCCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGt                                    <  2:1208006/140‑1 (MQ=255)                                                                                                      gATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGt                                <  2:708917/140‑1 (MQ=255)                                                                                                      gATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGt                                <  2:598045/140‑1 (MQ=255)                                                                                                              gCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAg                        >  2:698469/1‑140 (MQ=255)                                                                                                                          tgtCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCg            >  2:232163/1‑140 (MQ=255)                                                                                                                               cgGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGc       >  2:1199663/1‑140 (MQ=255)                                                                                                                               cgGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGc       >  1:594178/1‑140 (MQ=255)                                                                                                                                    aGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGt  >  1:1112198/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         TGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGT  >  NC_000913/3923794‑3924064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑