Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,965,207	T→C	T142A (ACG→GCG)	rhlB ←	ATP‑dependent RNA helicase RhlB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,965,207	0	T	C	100.0%	256.1 / NA	68	T142A (ACG→GCG)	rhlB	ATP‑dependent RNA helicase RhlB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/31);  total (37/31)

GGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGC  >  NC_000913/3965071‑3965342                                                                                                                                         |                                                                                                                                        ggcATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgcggt                                                                                                                                      >  2:966985/1‑140 (MQ=255) ggcATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgcggt                                                                                                                                      >  1:797771/1‑140 (MQ=255) ggcATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCgcggt                                                                                                                                      <  2:405764/140‑1 (MQ=255)                  ccagcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCaa                                                                                                                     <  2:1010753/140‑1 (MQ=255)                  ccagcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTAAATTAAACGCCCCGCGGTGCCAATCAGAATGTCaa                                                                                                                     <  1:983697/140‑1 (MQ=255)                    agcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAAcg                                                                                                                   <  2:1066616/140‑1 (MQ=255)                     gcGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAAcgc                                                                                                                  <  1:607542/140‑1 (MQ=255)                            tCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTc                                                                                                           >  2:135843/1‑140 (MQ=255)                              tttAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCa                                                                                                         <  1:799920/140‑1 (MQ=255)                                      aGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTc                                                                                                 >  2:914970/1‑140 (MQ=255)                                       gCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCa                                                                                                <  1:914970/140‑1 (MQ=255)                                        cccAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAg                                                                                               >  2:258951/1‑140 (MQ=255)                                             aTCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGtt                                                                                          >  1:245537/1‑140 (MQ=255)                                                 tACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTc                                                                                      >  2:1154302/1‑140 (MQ=255)                                                  aCATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCg                                                                                     >  1:341794/1‑140 (MQ=255)                                                  aCATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCg                                                                                     <  2:46852/140‑1 (MQ=255)                                                  aCATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCg                                                                                     >  2:187137/1‑140 (MQ=255)                                                    aTGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTa                                                                                   <  1:966985/140‑1 (MQ=255)                                                    aTGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTa                                                                                   <  2:734752/140‑1 (MQ=255)                                                      gCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGc                                                                                 >  1:669693/1‑140 (MQ=255)                                                       cGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGcc                                                                                >  2:280104/1‑140 (MQ=255)                                                       cGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGcc                                                                                >  1:1225311/1‑140 (MQ=255)                                                         aTCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCg                                                                                     <  1:712724/133‑1 (MQ=255)                                                         aTCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCg                                                                                     >  2:712724/1‑133 (MQ=255)                                                         aTCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCAt                                                                              >  1:954876/1‑140 (MQ=255)                                                         aTCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCAt                                                                              >  2:513397/1‑140 (MQ=255)                                                         aTCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCAt                                                                              >  2:921055/1‑140 (MQ=255)                                                         aTCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCAt                                                                              >  1:1041958/1‑140 (MQ=255)                                                         aTCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAAAGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCAt                                                                              >  2:370745/1‑140 (MQ=255)                                                           cGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATca                                                                            >  2:772431/1‑140 (MQ=255)                                                           cGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATca                                                                            <  2:172548/140‑1 (MQ=255)                                                            ggCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATcac                                                                           <  2:245537/140‑1 (MQ=255)                                                            ggCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATcac                                                                           >  2:304839/1‑140 (MQ=255)                                                            ggCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATcac                                                                           <  2:67881/140‑1 (MQ=255)                                                              cTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCAt                                                                              <  1:914416/135‑1 (MQ=255)                                                              cTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCAt                                                                              >  2:914416/1‑135 (MQ=255)                                                                tCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCaccacc                                                                       <  1:304839/140‑1 (MQ=255)                                                                 cGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCTATCACCACCg                                                                      >  1:203871/1‑140 (MQ=255)                                                                  gTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGt                                                                     <  1:1154302/140‑1 (MQ=255)                                                                      aGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGc                                                                 <  1:398185/140‑1 (MQ=255)                                                                             ccaccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAGCGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGAccc                                                          >  2:944658/1‑140 (MQ=255)                                                                             ccaccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGAccc                                                          >  2:503407/1‑140 (MQ=255)                                                                             ccaccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGAccc                                                          >  1:1070054/1‑140 (MQ=255)                                                                             ccaccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGAccc                                                          >  1:83759/1‑140 (MQ=255)                                                                             ccaccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGAccc                                                          <  1:503407/140‑1 (MQ=255)                                                                             ccaccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGAccc                                                          >  1:232950/1‑140 (MQ=255)                                                                                ccTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGc                                                       >  2:470551/1‑140 (MQ=255)                                                                                  tGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGc                                                                 <  1:399307/128‑1 (MQ=255)                                                                                  tGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGc                                                                 >  2:399307/1‑128 (MQ=255)                                                                                  tGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCtt                                                     <  2:1070054/140‑1 (MQ=255)                                                                                  tGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCtt                                                     <  1:92292/140‑1 (MQ=255)                                                                                  tGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCtt                                                     >  1:484645/1‑140 (MQ=255)                                                                                    aaTGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCa                                                   <  2:232950/140‑1 (MQ=255)                                                                                        gCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGcc                                               >  1:1113050/1‑140 (MQ=255)                                                                                         cACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCa                                              >  1:716744/1‑140 (MQ=255)                                                                                          aCCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAg                                             >  2:215913/1‑140 (MQ=255)                                                                                                       tgGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAg                                <  2:969534/140‑1 (MQ=255)                                                                                                         gTTCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCg                              >  1:1183625/1‑140 (MQ=255)                                                                                                           tCTGCTTGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGt                            >  2:269013/1‑140 (MQ=255)                                                                                                                 tGGCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCg                      <  2:1218895/140‑1 (MQ=255)                                                                                                                  ggCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCTATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGt                     <  2:203871/140‑1 (MQ=255)                                                                                                                  ggCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGt                     <  2:1113050/140‑1 (MQ=255)                                                                                                                  ggCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGt                     <  1:921055/140‑1 (MQ=255)                                                                                                                  ggCGTAGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGt                     <  2:954876/140‑1 (MQ=255)                                                                                                                       aGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCa                <  2:669693/140‑1 (MQ=255)                                                                                                                       aGTCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCa                <  1:135843/140‑1 (MQ=255)                                                                                                                         tCAATTAAACGCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATg              >  2:918143/1‑140 (MQ=255)                                                                                                                                   gCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGa            <  2:1093750/132‑1 (MQ=255)                                                                                                                                   gCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGa            >  1:1093750/1‑132 (MQ=255)                                                                                                                                   gCCCCGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACg    >  2:171693/1‑140 (MQ=255)                                                                                                                                     cccGCGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGc  >  1:139525/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACCACCACCTGAATGGCACCGAGGTTAATGTGGTTCTGCTTGGCGTAGTCAATTAAACGCCCCGTGGTGCCAATCAGAATGTCAACGCCGCTTTCCAGCACTTTCAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGC  >  NC_000913/3965071‑3965342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑