Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,976,801	C→T	A175V (GCG→GTG)	wzxE →	lipid III flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,976,801	0	C	T	100.0%	209.8 / NA	64	A175V (GCG→GTG)	wzxE	lipid III flippase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/29);  total (35/29)

GCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAG  >  NC_000913/3976665‑3976930                                                                                                                                         |                                                                                                                                  gCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGtt                                                                                                                                <  1:473984/140‑1 (MQ=255)  ccTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTg                                                                                                                               >  1:585750/1‑140 (MQ=255)    tGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCt                                                                                                                             <  1:687523/140‑1 (MQ=255)          aaCCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCt                                                                                                                       <  1:770422/140‑1 (MQ=255)          aaCCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCt                                                                                                                       <  1:945701/140‑1 (MQ=255)           aCCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTg                                                                                                                      >  2:1004418/1‑140 (MQ=255)           aCCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTg                                                                                                                      >  2:1000885/1‑140 (MQ=255)           aCCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTg                                                                                                                      >  2:201301/1‑140 (MQ=255)            ccTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTgg                                                                                                                     >  1:862303/1‑140 (MQ=255)                 tACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTg                                                                                                                >  2:1202630/1‑140 (MQ=255)                      gcgcTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTcc                                                                                                           >  2:826478/1‑140 (MQ=255)                      gcgcTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTcc                                                                                                           >  1:226635/1‑140 (MQ=255)                      gcgcTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTcc                                                                                                           >  2:611133/1‑140 (MQ=255)                      gcgcTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTcc                                                                                                           >  1:842582/1‑140 (MQ=255)                      gcgcTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTcc                                                                                                           >  2:734430/1‑140 (MQ=255)                       cgcTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTccc                                                                                                          >  2:622194/1‑140 (MQ=255)                        gcTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCcg                                                                                                         >  2:1050624/1‑140 (MQ=255)                        gcTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCcg                                                                                                         >  1:98857/1‑140 (MQ=255)                          tgatgaAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCcgcg                                                                                                       <  1:1211208/140‑1 (MQ=255)                          tgatgaAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCcgcg                                                                                                       >  1:811680/1‑140 (MQ=255)                          tgatgaAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCcgcg                                                                                                       <  2:742377/140‑1 (MQ=255)                            atgaAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCt                                                                                                     <  1:826478/140‑1 (MQ=255)                             tgaAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCtg                                                                                                    <  2:811680/140‑1 (MQ=255)                             tgaAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCtg                                                                                                    <  2:754448/140‑1 (MQ=255)                                      ttCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTTGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTAGTGGTAAtt                                                                                           >  2:846342/1‑140 (MQ=255)                                      ttCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAAtt                                                                                           >  2:897223/1‑140 (MQ=255)                                             tGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTgccg                                                                                    <  1:201301/140‑1 (MQ=255)                                               ccGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGCGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTgccgcc                                                                                  <  1:1120612/140‑1 (MQ=255)                                                           cGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATc                                                                      <  1:90894/140‑1 (MQ=255)                                                            gTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCa                                                                     <  1:398360/140‑1 (MQ=255)                                                            gTTATCGCTGATTGTCGGAAGCTTGATTGGCGTACTCGCGTAATACGTCAGTTACCGGTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCAGCAGCCATCATGTTGATCa                                                                     <  2:1182772/140‑1 (MQ=255)                                                             ttATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCaa                                                                    <  1:1004418/140‑1 (MQ=255)                                                               aTCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCca                                                                                 >  2:1219327/1‑125 (MQ=255)                                                               aTCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCca                                                                                 <  1:1219327/125‑1 (MQ=255)                                                               aTCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGAt                                                                       >  2:760725/1‑135 (MQ=255)                                                               aTCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGAt                                                                       <  1:760725/135‑1 (MQ=255)                                                               aTCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAAc                                                                  >  1:1157565/1‑140 (MQ=255)                                                               aTCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAAc                                                                  <  2:1167076/140‑1 (MQ=255)                                                               aTCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAAc                                                                  >  1:614882/1‑140 (MQ=255)                                                                     gATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACgtggtg                                                            >  2:510984/1‑140 (MQ=255)                                                                     gATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACgtggtg                                                            >  2:229596/1‑140 (MQ=255)                                                                        tGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCa                                                         >  1:216868/1‑140 (MQ=255)                                                                        tGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCa                                                         <  1:1202630/140‑1 (MQ=255)                                                                        tGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCa                                                         <  2:1084545/140‑1 (MQ=255)                                                                             gCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCg                                                    >  2:173352/1‑140 (MQ=255)                                                                                 cTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTaa                                                >  1:603101/1‑140 (MQ=255)                                                                                           gTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGaa                                      >  1:812614/1‑140 (MQ=255)                                                                                           gTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGaa                                      >  1:1117591/1‑140 (MQ=255)                                                                                                cgcgTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCa                                 <  1:443440/140‑1 (MQ=255)                                                                                                  cgTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGc                               <  2:585750/140‑1 (MQ=255)                                                                                                   gTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCt                              <  1:734430/140‑1 (MQ=255)                                                                                                        acGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGAt                         <  2:842582/140‑1 (MQ=255)                                                                                                        acGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGAt                         <  2:226635/140‑1 (MQ=255)                                                                                                         cGTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATa                        >  1:588157/1‑140 (MQ=255)                                                                                                          gTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATaa                       <  2:1117591/140‑1 (MQ=255)                                                                                                          gTCAGTTACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATaa                       <  1:229596/140‑1 (MQ=255)                                                                                                               ttACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTc                  >  2:947119/1‑140 (MQ=255)                                                                                                               ttACCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTc                  <  1:1017132/140‑1 (MQ=255)                                                                                                                 aCCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTg                >  2:607613/1‑140 (MQ=255)                                                                                                                 aCCGTTTGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTg                >  2:1059687/1‑140 (MQ=255)                                                                                                                     tttGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCt                              <  2:680040/122‑1 (MQ=255)                                                                                                                     tttGGGCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCt                              >  1:680040/1‑122 (MQ=255)                                                                                                                        gggCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTggcagggc         >  1:464937/1‑140 (MQ=255)                                                                                                                         ggCGGTTATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTggcagggca        >  1:751357/1‑140 (MQ=255)                                                                                                                               tATGAAGGGGTGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAg  <  2:603101/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  GCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAG  >  NC_000913/3976665‑3976930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑