Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,081,038	T→C	Q64R (CAG→CGG)	fdhE ←	formate dehydrogenase formation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,081,038	0	T	C	100.0%	205.3 / NA	55	Q64R (CAG→CGG)	fdhE	formate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/22);  total (33/22)

CGCCATCAGCAGCTTTTGCCAGTGCTTATCACGCGGCAGAACGTGAATATCCAGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCTGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCC  >  NC_000913/4080902‑4081172                                                                                                                                         |                                                                                                                                       cgcCATCAGCAGCTTTTGCCAGTGCTTATCACGCGGCAGAACGTGAATATCCAGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCcggg                                                                                                                                     >  1:318131/1‑140 (MQ=255)   ccATCAGCAGCTTTTGCCAGTGCTTATCACGCGGCAGAACGTGAATATCCAGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCcgggcg                                                                                                                                   <  2:17693/140‑1 (MQ=255)      tCAGCAGCTTTTGCCAGTGCTTATCACGCGGCAGAACGTGAATATCCAGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCcgggcgtgg                                                                                                                                <  1:435709/140‑1 (MQ=255)             cTTTTGCCAGTGCTTATCACGCGGCAGAACGTGAATATCCAGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATaa                                                                                                                         >  1:16849/1‑140 (MQ=255)                   ccAGTGCTTATCACGCGGCAGAACGTGAATATCCAGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCgcag                                                                                                                   <  1:55911/140‑1 (MQ=255)                        gCTTATCACGCGGCAGAACGTGAATATCCAGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAACAAAg                                                                                                              <  1:288799/140‑1 (MQ=255)                           tATCACGCGGCAGAACGTGAATATCCAGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAgcgc                                                                                                           <  1:773577/140‑1 (MQ=255)                            aTCACGCGGCAGAACGTGAATATCCAGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCa                                                                                                          <  2:396474/140‑1 (MQ=255)                                 gcgGCAGAACGTGAATATCCAGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGTACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTaa                                                                                                     >  1:398913/1‑140 (MQ=255)                                   ggCAGAACGTGAATATCCAGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATc                                                                                                   >  2:288413/1‑140 (MQ=255)                                   ggCAGAACGTGAATATCCAGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATc                                                                                                   >  2:1250091/1‑140 (MQ=255)                                              aaTATCCAGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGat                                                                                        <  2:1047049/140‑1 (MQ=255)                                                     aGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTc                                                                                 >  1:1182898/1‑140 (MQ=255)                                                       cGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTg                                                                               >  1:693665/1‑140 (MQ=255)                                                          gggCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCa                                                                            <  2:612657/140‑1 (MQ=255)                                                            gCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCACAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGc                                                                          >  2:1168554/1‑140 (MQ=255)                                                               tGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTcg                                                                       <  1:288413/140‑1 (MQ=255)                                                                      tgCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGAc                                                                >  1:1280647/1‑140 (MQ=255)                                                                      tgCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGAc                                                                >  2:842938/1‑140 (MQ=255)                                                                         gcTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCt                                                             <  2:693665/140‑1 (MQ=255)                                                                          cTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTc                                                            >  1:1167668/1‑140 (MQ=255)                                                                           tGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTcg                                                           >  1:898249/1‑140 (MQ=255)                                                                                    tAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGcggcgg                                                  <  1:387441/140‑1 (MQ=255)                                                                                    tAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGcggcgg                                                  <  1:1250091/140‑1 (MQ=255)                                                                                         cgcgcAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGta                                             >  1:102504/1‑140 (MQ=255)                                                                                           cgcAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGtata                                           >  1:185103/1‑140 (MQ=255)                                                                                           cgcAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGCTggtta                                           >  2:483819/1‑136 (MQ=255)                                                                                           cgcAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGCTGttta                                           >  1:589927/1‑140 (MQ=255)                                                                                                      ccATCTCCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGc                                <  2:16849/140‑1 (MQ=255)                                                                                                         tctcCAGCGGATGGTCGTACAGTACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGa                             <  2:398913/140‑1 (MQ=255)                                                                                                         tctcCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGa                             <  2:806374/140‑1 (MQ=255)                                                                                                         tctcCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGa                             >  1:443119/1‑140 (MQ=255)                                                                                                          ctcCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTTAGCCGAg                            >  2:286863/1‑140 (MQ=255)                                                                                                          ctcCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAg                            >  1:131662/1‑140 (MQ=255)                                                                                                          ctcCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAg                            >  1:1168126/1‑140 (MQ=255)                                                                                                          ctcCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAg                            >  2:668156/1‑140 (MQ=255)                                                                                                          ctcCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAg                            >  1:885375/1‑140 (MQ=255)                                                                                                          ctcCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAAACGCTCGGCGCGGCGGTTGtata                                           >  1:446703/1‑125 (MQ=255)                                                                                                          ctcCAGCGGATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAAACGCTCGGCGCGGCGGTTGtata                                           <  2:446703/125‑1 (MQ=255)                                                                                                                  gATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACaaa                    >  1:1031677/1‑139 (MQ=255)                                                                                                                  gATGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAAt                    <  1:1020134/140‑1 (MQ=255)                                                                                                                   aTGGTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATa                   >  2:938404/1‑140 (MQ=255)                                                                                                                     ggTCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAAc                 >  1:475272/1‑140 (MQ=255)                                                                                                                       tCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGa                             <  2:946813/126‑1 (MQ=255)                                                                                                                       tCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGa                             >  1:946813/1‑126 (MQ=255)                                                                                                                       tCGTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAAcgg               <  2:318131/140‑1 (MQ=255)                                                                                                                         gTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAAcggcg             <  2:12156/140‑1 (MQ=255)                                                                                                                         gTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAAcggcg             <  1:938404/140‑1 (MQ=255)                                                                                                                         gTACAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAAcggcg             <  1:668156/140‑1 (MQ=255)                                                                                                                           aCAGCACCACTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGa           <  2:131662/140‑1 (MQ=255)                                                                                                                               caccacTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCa       >  1:754694/1‑140 (MQ=255)                                                                                                                               caccacTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCa       >  2:860617/1‑140 (MQ=255)                                                                                                                               caccacTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGGTGGATAAATTCTTGGACCGAGGGGACAATAAACGCGGAAACa       >  2:651897/1‑140 (MQ=255)                                                                                                                                 ccacTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGGACAATAAaggcgggacatat     >  2:290262/1‑133 (MQ=255)                                                                                                                                 ccacTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGGACAATAACGGCGGGAACata     >  2:1213912/1‑140 (MQ=255)                                                                                                                                 ccacTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCata     >  2:429637/1‑140 (MQ=255)                                                                                                                                  cacTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCatat    <  1:1074977/140‑1 (MQ=255)                                                                                                                                    cTTCCCGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTTTTGAGCCGAGGGAACAATAACGGCGGGAACATATcc  >  2:70414/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CGCCATCAGCAGCTTTTGCCAGTGCTTATCACGCGGCAGAACGTGAATATCCAGCGGGGGCTTGCCTTGTGCGCTGGCTTCTTTAATGCGCGCAGTCAGATCCATCTCCAGCGGATGGTCGTACAGCACCACTTCCTGGGCGTGGGCGATAAGCGCAGCAAAGCGCAGGTAATCACCCAGAGGATTATTTTCTGCCAGCTCGCGCAGACGCTCGGCGCGGCGGTTGTATAAATTCTTGAGCCGAGGGAACAATAACGGCGGAATCATATCC  >  NC_000913/4080902‑4081172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑