Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,632,782	C→T	S8L (TCA→TTA)	trpR →	DNA‑binding transcriptional repressor TrpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,632,782	0	C	T	100.0%	313.7 / NA	87	S8L (TCA→TTA)	trpR	DNA‑binding transcriptional repressor TrpR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/45);  total (42/45)

CGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGACT  >  NC_000913/4632648‑4632918                                                                                                                                       |                                                                                                                                         cGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTtagcag                                                                                                                                     >  2:598911/1‑140 (MQ=255)          gACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAg                                                                                                                            <  2:10235/140‑1 (MQ=255)           aCGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCAACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGa                                                                                                                           <  1:60566/140‑1 (MQ=255)             gtcgtTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAAc                                                                                                                         >  2:692373/1‑140 (MQ=255)               cgtTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACC‑GGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGc                                                                                                                      <  1:1020255/140‑1 (MQ=255)                gtTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGc                                                                                                                      <  2:893565/140‑1 (MQ=255)                       aTCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCa                                                                                                               <  2:349369/140‑1 (MQ=255)                       aTCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCa                                                                                                               >  1:349369/1‑140 (MQ=255)                        tCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGc                                                                                                                      <  2:141510/132‑1 (MQ=255)                        tCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGc                                                                                                                      >  1:141510/1‑132 (MQ=255)                         ccGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAgg                                                                                                             >  2:822395/1‑140 (MQ=255)                         ccGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAgg                                                                                                             <  1:692373/140‑1 (MQ=255)                         ccGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAgg                                                                                                             >  2:1129253/1‑140 (MQ=255)                          cGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGa                                                                                                            <  1:950233/140‑1 (MQ=255)                          cGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGa                                                                                                            <  2:872953/140‑1 (MQ=255)                           gCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAg                                                                                                           <  1:1104194/140‑1 (MQ=255)                           gCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAg                                                                                                           >  2:562569/1‑140 (MQ=255)                                 ttATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTa                                                                                                     >  2:462375/1‑140 (MQ=255)                                   aTGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACg                                                                                                   <  1:780154/140‑1 (MQ=255)                                         tGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGt                                                                                             >  1:1261184/1‑140 (MQ=255)                                         tGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGt                                                                                             >  2:423617/1‑140 (MQ=255)                                         tGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGt                                                                                             >  2:102792/1‑140 (MQ=255)                                         tGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGt                                                                                             >  2:514941/1‑140 (MQ=255)                                           cTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCg                                                                                           >  1:409701/1‑140 (MQ=255)                                             aTCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAc                                                                                         <  1:598911/140‑1 (MQ=255)                                             aTCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAc                                                                                         <  2:346763/140‑1 (MQ=255)                                             aTCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAc                                                                                         <  1:653983/140‑1 (MQ=255)                                             aTCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAc                                                                                         <  1:68919/140‑1 (MQ=255)                                             aTCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAc                                                                                         <  1:822395/140‑1 (MQ=255)                                             aTCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAc                                                                                         <  1:1009109/140‑1 (MQ=255)                                             aTCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAc                                                                                         <  2:409701/140‑1 (MQ=255)                                             aTCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAc                                                                                         <  1:102792/140‑1 (MQ=255)                                             aTCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAc                                                                                         <  2:856667/140‑1 (MQ=255)                                             aTCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAc                                                                                         <  1:269002/140‑1 (MQ=255)                                              tCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAcc                                                                                        >  2:501257/1‑140 (MQ=255)                                               cGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCt                                                                                       >  2:216437/1‑140 (MQ=255)                                                gTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTg                                                                                      <  1:717195/140‑1 (MQ=255)                                                   ctctTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCtt                                                                                   >  2:549199/1‑140 (MQ=255)                                                   ctctTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCtt                                                                                   >  1:1238139/1‑140 (MQ=255)                                                   ctctTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCtt                                                                                   >  1:980038/1‑140 (MQ=255)                                                    tctTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTa                                                                                  <  1:168772/140‑1 (MQ=255)                                                     ctTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTaa                                                                                 >  2:712631/1‑140 (MQ=255)                                                     ctTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTATCGACCTGCTTaa                                                                                 >  2:766557/1‑140 (MQ=255)                                                      tttAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAg                                                                                >  1:775981/1‑140 (MQ=255)                                                        tAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGaa                                                                              >  1:878102/1‑140 (MQ=255)                                                         aGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAAt                                                                             <  2:933450/140‑1 (MQ=255)                                                           cGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAc                                                                                         <  1:5510/126‑1 (MQ=255)                                                           cGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGAc                                                                                         >  2:5510/1‑126 (MQ=255)                                                             aGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCt                                                                         >  2:311939/1‑140 (MQ=255)                                                              gTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTa                                                                        >  2:270774/1‑140 (MQ=255)                                                               tACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTAc                                                                       >  1:1168431/1‑140 (MQ=255)                                                               tACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTAc                                                                       >  1:746289/1‑140 (MQ=255)                                                                aCAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTAcc                                                                      <  1:545363/140‑1 (MQ=255)                                                                aCAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTAcc                                                                      <  1:1129253/140‑1 (MQ=255)                                                                aCAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTAcc                                                                      >  1:403268/1‑140 (MQ=255)                                                                  aaCCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCaa                                                                    >  2:964482/1‑140 (MQ=255)                                                                        gggAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCCCCCCATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGGCCTGCTTAAGAATGCCTACCAAAACGAt                                                              <  2:103053/140‑1 (MQ=255)                                                                        gggAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGAt                                                              <  1:501257/140‑1 (MQ=255)                                                                        gggAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGAt                                                              >  1:1142846/1‑140 (MQ=255)                                                                         ggAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGAtc                                                             >  2:739425/1‑140 (MQ=255)                                                                         ggAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGAtc                                                             >  2:1204257/1‑140 (MQ=255)                                                                         ggAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGAtc                                                             <  1:964482/140‑1 (MQ=255)                                                                               aTTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCAtt                                                       <  1:51190/140‑1 (MQ=255)                                                                                       tCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCgttg                                               >  2:949108/1‑140 (MQ=255)                                                                                           ccGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTaa                                           >  2:438243/1‑140 (MQ=255)                                                                                              cTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAAcc                                        >  1:1053305/1‑140 (MQ=255)                                                                                                aaCAATGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTg                                      <  1:514941/140‑1 (MQ=255)                                                                                                   aaTGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATg                                   <  2:895124/140‑1 (MQ=255)                                                                                                     tGGCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCt                                 <  2:1238139/140‑1 (MQ=255)                                                                                                       gCGACATATTATGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCGCCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGa                               <  2:1261184/140‑1 (MQ=255)                                                                                                               ttatGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGAt                       <  2:775981/140‑1 (MQ=255)                                                                                                                 atGGCCCAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGa                     <  2:1168431/140‑1 (MQ=255)                                                                                                                     cccAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAgcgc                 >  1:1168308/1‑140 (MQ=255)                                                                                                                      ccAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGcgcg                <  1:721124/140‑1 (MQ=255)                                                                                                                      ccAACAATCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGcgcg                <  2:1168308/140‑1 (MQ=255)                                                                                                                       caacaaTCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGa               >  1:1016797/1‑140 (MQ=255)                                                                                                                       caacaaTCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGa               >  2:26565/1‑140 (MQ=255)                                                                                                                        aacaaTCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGaa              <  1:216437/140‑1 (MQ=255)                                                                                                                        aacaaTCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGaa              <  2:527796/140‑1 (MQ=255)                                                                                                                        aacaaTCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGaa              >  1:527796/1‑140 (MQ=255)                                                                                                                         acaaTCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAg             <  1:462375/140‑1 (MQ=255)                                                                                                                         acaaTCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAg             >  1:204287/1‑140 (MQ=255)                                                                                                                         acaaTCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAg             >  2:535379/1‑140 (MQ=255)                                                                                                                           aaTCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCg           <  2:204287/140‑1 (MQ=255)                                                                                                                             tCACCCTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGtt         >  1:871509/1‑140 (MQ=255)                                                                                                                                 ccTATTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTgggg     >  1:361314/1‑140 (MQ=255)                                                                                                                                    aTTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGACt  <  1:311939/140‑1 (MQ=255)                                                                                                                                    aTTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGACt  <  1:423617/140‑1 (MQ=255)                                                                                                                                    aTTTAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGACt  <  1:1078242/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGCCCAACAATCACCCTATTCAGCAGCGATGGCAGAACAGCGTCACCAGGAGTGGTTACGTTTTGTCGACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCGCGAAGCGTTGGGGACT  >  NC_000913/4632648‑4632918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑