Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	413,751	G→A	A668V (GCG→GTG)	sbcC ←	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	413,751	0	G	A	100.0%	190.8 / NA	54	A668V (GCG→GTG)	sbcC	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/27);  total (27/27)

CAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATAATTTGCTGATTATGCGCGGCAATCTGCC  >  NC_000913/413614‑413886                                                                                                                                          |                                                                                                                                        caGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCAcc                                                                                                                                       >  1:1257573/1‑140 (MQ=255)          cGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGctc                                                                                                                              >  1:135579/1‑140 (MQ=255)             aGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGctctct                                                                                                                           >  1:995558/1‑140 (MQ=255)              gTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCtctt                                                                                                                          <  1:1035075/140‑1 (MQ=255)              gTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCtctt                                                                                                                          >  2:387115/1‑140 (MQ=255)               tGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCtcttc                                                                                                                         >  2:55471/1‑140 (MQ=255)                gCGGGTGTTCATCACTTTGCGGCAACGTTTCCATAATCGGCGTCAGCTGTTGTATACGATTTTGCAGCGCGATTAATTCGTTCTGGCGTTGATGCCTGCTCTGCACTTCTTGATGACGTGGCACCAACCAGCTCactact                                                                                                                        <  2:521418/140‑1 (MQ=255)                        tcatcaCTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTc                                                                                                                <  1:311841/140‑1 (MQ=255)                         catcaCTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCAt                                                                                                                    <  2:251764/135‑1 (MQ=255)                         catcaCTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCAt                                                                                                                    >  1:251764/1‑135 (MQ=255)                         catcaCTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTcc                                                                                                               >  2:729609/1‑140 (MQ=255)                         catcaCTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTcc                                                                                                               >  1:580945/1‑140 (MQ=255)                               tttGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGc                                                                                                         <  2:135579/140‑1 (MQ=255)                                 tGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCaa                                                                                                       >  1:918633/1‑140 (MQ=255)                                   cGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATg                                                                                                     >  2:20986/1‑140 (MQ=255)                                    ggCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGt                                                                                                    <  2:1257573/140‑1 (MQ=255)                                    ggCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGt                                                                                                    <  1:264824/140‑1 (MQ=255)                                    ggCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGt                                                                                                    <  1:20986/140‑1 (MQ=255)                                     gCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTTGCAATGTc                                                                                                   <  2:580945/140‑1 (MQ=255)                                     gCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTGCTTCATCTTCCTGTGGCAATGTc                                                                                                   <  2:11625/140‑1 (MQ=255)                                         cGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTg                                                                                               <  1:395469/140‑1 (MQ=255)                                         cGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTg                                                                                               >  2:1261068/1‑140 (MQ=255)                                         cGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTg                                                                                               <  1:729609/140‑1 (MQ=255)                                         cGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTg                                                                                               >  2:877240/1‑140 (MQ=255)                                           tttCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCATCCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCa                                                                                             >  1:1033952/1‑140 (MQ=255)                                            ttCCAGAATCGGCGTCAGCTGCTGATTACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCAt                                                                                            <  2:376281/140‑1 (MQ=255)                                            ttCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCAt                                                                                            <  2:484309/140‑1 (MQ=255)                                              ccAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATaa                                                                                          <  1:541070/140‑1 (MQ=255)                                                       gCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAAt                                                                                 <  1:183034/140‑1 (MQ=255)                                                       gCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAAt                                                                                 <  1:763164/140‑1 (MQ=255)                                                            aGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGt                                                                            >  2:250616/1‑140 (MQ=255)                                                              ctgctgAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTa                                                                          >  1:446226/1‑140 (MQ=255)                                                              ctgctgAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTa                                                                          >  2:187354/1‑140 (MQ=255)                                                                         ggTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGc                                                               <  1:55471/140‑1 (MQ=255)                                                                              tGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCg                                                          >  1:45832/1‑140 (MQ=255)                                                                                     cgGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCa                                                   >  2:183519/1‑140 (MQ=255)                                                                                         tAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAAttt                                               <  2:995558/140‑1 (MQ=255)                                                                                         tAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAAttt                                               <  1:1254978/140‑1 (MQ=255)                                                                                          aaTTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTg                                              <  1:1261068/140‑1 (MQ=255)                                                                                              cGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGt                                          <  2:446226/140‑1 (MQ=255)                                                                                                ttCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTg                                              >  2:629743/1‑134 (MQ=255)                                                                                                ttCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTg                                              <  1:629743/134‑1 (MQ=255)                                                                                                  cTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGAt                                      <  1:183519/140‑1 (MQ=255)                                                                                                          gctgcCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATa                              >  2:138245/1‑140 (MQ=255)                                                                                                           ctgcCAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTTGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATaa                             >  2:1145085/1‑140 (MQ=255)                                                                                                               cAGCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATAATTTg                         >  1:852729/1‑140 (MQ=255)                                                                                                                 gCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATAATTTg                         <  1:852713/138‑1 (MQ=255)                                                                                                                 gCTCTGCGCTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATAATTTg                         >  2:852713/1‑138 (MQ=255)                                                                                                                   tctGCGCTTCTTGCTGACGTGTCACCATCCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATAATTTGCTGa                     <  2:1033952/140‑1 (MQ=255)                                                                                                                       cgcTTCTTGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATAATTTGCTGATTAt                 >  2:1268565/1‑140 (MQ=255)                                                                                                                           tcttGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATAATTTGCTGATTATgcgc             >  1:847672/1‑140 (MQ=255)                                                                                                                            cttGCTGACGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATAATTTGCTGATTATGcgcg            >  2:446300/1‑140 (MQ=255)                                                                                                                                    cGTGTCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATAATTTGCTGATTATGCGCGGCAATCTg    <  1:250616/140‑1 (MQ=255)                                                                                                                                     gtgtCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATAATTTGCTGATTATGCGCGGCAATCTGc   <  1:877240/140‑1 (MQ=255)                                                                                                                                     gtgtCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATAATTTGCTGATTATGCGCGGCAATCTGc   <  1:101830/140‑1 (MQ=255)                                                                                                                                      tgtCACCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATAATTTGCTGATTATGCGCGGCAATCTGcc  >  2:138624/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CAGTTTCTTCGCAGTGCGGGAGTTCATCACTTTGCGGCAACGTTTCCAGAATCGGCGTCAGCTGCTGAATACGGTTTTGCAGCGCGGTTAATTCGTTCTGGCGTTGCTGCCAGCTCTGCGCTTCTTGCTGACGTGTCGCCAACCAGCTCTCTTCTTCATCTTCCTGTGGCAATGTCAGTGCATAACCCGTCAATGTCGTTAAAAGTAGTTGCTGGCGTTGTTCAATTTGCTGTTGATACTGGATAATTTGCTGATTATGCGCGGCAATCTGCC  >  NC_000913/413614‑413886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑