Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	430,579	C→T	intergenic (+103/‑26)	secF → / → yajD	Sec translocon accessory complex subunit SecF/HNH nuclease family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	430,579	0	C	T	100.0%	157.4 / NA	45	intergenic (+103/‑26)	secF/yajD	Sec translocon accessory complex subunit SecF/HNH nuclease family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/19);  total (26/19)

AAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTA  >  NC_000913/430442‑430711                                                                                                                                          |                                                                                                                                     aagaagGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAActct                                                                                                                                    <  2:706559/140‑1 (MQ=255)               cAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAgg                                                                                                                      <  1:863242/140‑1 (MQ=255)                  ccGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTaa                                                                                                                   <  2:276676/140‑1 (MQ=255)                          tCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCAt                                                                                                           <  2:1270733/140‑1 (MQ=255)                                gTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTat                                                                                                     <  1:583483/140‑1 (MQ=255)                                  aaTCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTatca                                                                                                   <  2:850671/140‑1 (MQ=255)                                   aTCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTatca                                                                                                   <  2:693712/139‑1 (MQ=255)                                   aTCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTatca                                                                                                   >  1:693712/1‑139 (MQ=255)                                    tCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTatcatc                                                                                                 <  1:686957/140‑1 (MQ=255)                                        gTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCaa                                                                                             >  2:1032089/1‑140 (MQ=255)                                         ttCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCaaa                                                                                            >  2:736226/1‑140 (MQ=255)                                             cGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAAc                                                                                        >  1:411444/1‑140 (MQ=255)                                             cGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGCAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAAc                                                                                        >  1:888822/1‑140 (MQ=255)                                                  aTGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATgc                                                                                   >  1:579761/1‑140 (MQ=255)                                                      tGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCgg                                                                               >  2:1052808/1‑140 (MQ=255)                                                       gAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAAGTAACTTTTCATGGCTATCATCCCAAAAAACAATGCGCGGt                                                                              >  1:506729/1‑140 (MQ=255)                                                                ggTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCg                                                                     >  1:740346/1‑140 (MQ=255)                                                                 gTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCgg                                                                    >  1:647308/1‑140 (MQ=255)                                                                 gTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCgg                                                                    >  2:548425/1‑140 (MQ=255)                                                                 gTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCgg                                                                    <  2:810422/140‑1 (MQ=255)                                                                      aagaTCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATc                                                               >  2:326478/1‑140 (MQ=255)                                                                        gaTCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGt                                                                              >  1:747479/1‑123 (MQ=255)                                                                        gaTCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGt                                                                              <  2:747479/123‑1 (MQ=255)                                                                           cGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGaa                                                          >  2:760081/1‑140 (MQ=255)                                                                           cGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGaa                                                          >  2:988379/1‑140 (MQ=255)                                                                             ggATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGaaaa                                                        <  1:326478/140‑1 (MQ=255)                                                                                   tttttGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGTGAAAAAGCAtt                                                  <  1:736226/140‑1 (MQ=255)                                                                                   tttttGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCAtt                                                  <  1:1052808/140‑1 (MQ=255)                                                                                     tttGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaa                                                <  2:49283/140‑1 (MQ=255)                                                                                     tttGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaa                                                <  2:740346/140‑1 (MQ=255)                                                                                                gagaTTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCg                                     <  1:548425/140‑1 (MQ=255)                                                                                                 agaTTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGt                                    <  2:732609/140‑1 (MQ=255)                                                                                                   aTTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTgg                                  >  2:197260/1‑140 (MQ=255)                                                                                                    ttGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTggg                                 >  1:848073/1‑140 (MQ=255)                                                                                                     tGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGt                                >  2:231318/1‑140 (MQ=255)                                                                                                     tGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGt                                >  1:848226/1‑140 (MQ=255)                                                                                                      gCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTc                               >  1:659921/1‑140 (MQ=255)                                                                                                       cGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCt                              >  1:753510/1‑140 (MQ=255)                                                                                                                            aaaCTGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAgt         <  1:760081/140‑1 (MQ=255)                                                                                                                                tGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAgtttgt     <  2:753510/140‑1 (MQ=255)                                                                                                                                tGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAgtttgt     <  1:197260/140‑1 (MQ=255)                                                                                                                                tGCGCGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAG‑GGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAgtttgtt    >  1:206638/1‑140 (MQ=255)                                                                                                                                tGCGCGTAACTCTACATTTCATCCAAGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAgtttgt     <  2:506729/140‑1 (MQ=255)                                                                                                                                 gcgcGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAgtttgtt    >  1:897617/1‑140 (MQ=255)                                                                                                                                 gcgcGTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAgtttgtt    >  1:830401/1‑140 (MQ=255)                                                                                                                                  cgcgTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAgtttgttt   >  2:538410/1‑140 (MQ=255)                                                                                                                                   gcgTAACTCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTa  >  2:34153/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     AAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTA  >  NC_000913/430442‑430711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑