Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	464,281	A→G	E115E (GAA→GAG)	ybaV →	helix‑hairpin‑helix 3 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	464,281	0	A	G	100.0%	326.2 / NA	88	E115E (GAA→GAG)	ybaV	helix‑hairpin‑helix 3 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/47);  total (41/47)

AGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATC  >  NC_000913/464152‑464416                                                                                                                                  |                                                                                                                                        aGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTgg                                                                                                                               <  2:100010/140‑1 (MQ=255)  gCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGc                                                                                                                              <  1:458667/140‑1 (MQ=255)  gCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGc                                                                                                                              <  2:1222964/140‑1 (MQ=255)  gCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGc                                                                                                                              <  2:328253/140‑1 (MQ=255)    ccGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCgg                                                                                                                            <  1:995132/140‑1 (MQ=255)       cgcgATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTAt                                                                                                                         >  1:1047962/1‑140 (MQ=255)       cgcgATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTAt                                                                                                                         >  1:363163/1‑140 (MQ=255)       cgcgATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTAt                                                                                                                         >  2:903945/1‑140 (MQ=255)          gATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTaa                                                                                                                      <  1:700868/140‑1 (MQ=255)            tGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAAcc                                                                                                                    >  2:333364/1‑140 (MQ=255)            tGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAAcc                                                                                                                    <  2:358221/140‑1 (MQ=255)                  gCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGtaa                                                                                                              <  2:946004/140‑1 (MQ=255)                     ttGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGtaatta                                                                                                           >  1:764471/1‑140 (MQ=255)                             aagaaAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCa                                                                                                    >  2:573999/1‑139 (MQ=255)                             aagaaAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCa                                                                                                    <  1:573999/139‑1 (MQ=255)                             aagaaAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCAt                                                                                                   <  2:974369/140‑1 (MQ=255)                             aagaaAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCAt                                                                                                   >  1:584243/1‑140 (MQ=255)                             aagaaAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCAt                                                                                                   >  1:546511/1‑140 (MQ=255)                             aagaaAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCATAATCTGGCGGTATTAACCCTGTAATTAATTTGCAt                                                                                                   >  2:1003359/1‑140 (MQ=255)                                  aGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTgg                                                                                              >  1:648241/1‑140 (MQ=255)                                  aGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTgg                                                                                              >  2:1174419/1‑140 (MQ=255)                                  aGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTgg                                                                                              >  1:450567/1‑140 (MQ=255)                                   gcgcAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGc                                                                                             <  2:764471/140‑1 (MQ=255)                                   gcgcAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGc                                                                                             <  2:706622/140‑1 (MQ=255)                                       aGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAAtt                                                                                         >  1:314418/1‑140 (MQ=255)                                       aGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGACGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAAtt                                                                                         >  2:1126180/1‑140 (MQ=255)                                            aTTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTg                                                                                    >  1:321549/1‑140 (MQ=255)                                            aTTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTg                                                                                    <  1:1126180/140‑1 (MQ=255)                                            aTTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTg                                                                                    <  2:321549/140‑1 (MQ=255)                                             ttGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCATAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGc                                                                                   <  1:1003359/140‑1 (MQ=255)                                               gTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCa                                                                                 <  2:444664/140‑1 (MQ=255)                                                   gTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACt                                                                             >  1:404987/1‑140 (MQ=255)                                                      aTCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGaa                                                                          <  1:333364/140‑1 (MQ=255)                                                        cgcgAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATCAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAaga                                                                        <  2:68677/140‑1 (MQ=255)                                                          cgAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAgg                                                                      >  1:792446/1‑140 (MQ=255)                                                           gAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGt                                                                     <  2:546511/140‑1 (MQ=255)                                                           gAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGt                                                                     <  2:484672/140‑1 (MQ=255)                                                                gTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAc                                                                <  2:314418/140‑1 (MQ=255)                                                                gTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAc                                                                <  2:287470/140‑1 (MQ=255)                                                                 tACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAcc                                                               >  1:423468/1‑140 (MQ=255)                                                                   cGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAc                                                                >  1:561706/1‑137 (MQ=255)                                                                   cGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAc                                                                <  2:561706/137‑1 (MQ=255)                                                                       ccGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTAt                                                         <  2:976951/140‑1 (MQ=255)                                                                       ccGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTAt                                                         <  1:1174419/140‑1 (MQ=255)                                                                         gTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGa                                                       >  1:755381/1‑140 (MQ=255)                                                                          tttAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAc                                                      <  2:404987/140‑1 (MQ=255)                                                                          tttAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAc                                                      <  2:151468/140‑1 (MQ=255)                                                                           ttAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAcc                                                     >  2:1105/1‑140 (MQ=255)                                                                           ttAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAcc                                                     >  2:1222257/1‑140 (MQ=255)                                                                            tAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACct                                                    <  1:207945/140‑1 (MQ=255)                                                                              aaaCTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACctct                                                  <  2:1047962/140‑1 (MQ=255)                                                                              aaaCTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACctct                                                  <  2:584243/140‑1 (MQ=255)                                                                                  tgtgGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTAc                                              >  2:365620/1‑140 (MQ=255)                                                                                    tgGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACtt                                            >  2:54519/1‑140 (MQ=255)                                                                                    tgGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACtt                                            >  1:326739/1‑140 (MQ=255)                                                                                      gaggaTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTtat                                          <  1:1105/140‑1 (MQ=255)                                                                                          aTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaaca                                      <  1:1222257/140‑1 (MQ=255)                                                                                           tCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaa                                     <  1:132034/140‑1 (MQ=255)                                                                                                aGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACg                                <  2:145877/140‑1 (MQ=255)                                                                                                aGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACg                                <  2:176092/140‑1 (MQ=255)                                                                                                    ggTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAg                            <  2:648241/140‑1 (MQ=255)                                                                                                     gTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAgg                           >  2:730652/1‑140 (MQ=255)                                                                                                     gTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAgg                           >  2:8575/1‑140 (MQ=255)                                                                                                     gTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAgg                           >  2:859329/1‑140 (MQ=255)                                                                                                     gTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAgg                           <  2:111404/140‑1 (MQ=255)                                                                                                     gTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAgg                           >  2:1128244/1‑140 (MQ=255)                                                                                                     gTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAgg                           >  1:111404/1‑140 (MQ=255)                                                                                                     gTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAgg                           >  2:1085145/1‑140 (MQ=255)                                                                                                     gTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAgg                           >  1:1191213/1‑140 (MQ=255)                                                                                                     gTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAgg                           >  2:138424/1‑140 (MQ=255)                                                                                                     gTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAgg                           >  1:489796/1‑140 (MQ=255)                                                                                                     gTGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAgg                           >  1:193683/1‑140 (MQ=255)                                                                                                      tGCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGt                          >  2:15668/1‑140 (MQ=255)                                                                                                       gCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGtt                         >  2:454497/1‑140 (MQ=255)                                                                                                       gCCGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGtt                         >  2:698886/1‑140 (MQ=255)                                                                                                        ccGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTa                        <  2:450567/140‑1 (MQ=255)                                                                                                        ccGGGGATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTa                        <  1:8575/140‑1 (MQ=255)                                                                                                             gATGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTgcg                   <  2:489796/140‑1 (MQ=255)                                                                                                              aTGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTgcgc                  <  2:423468/140‑1 (MQ=255)                                                                                                              aTGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTgcgc                  <  2:755381/140‑1 (MQ=255)                                                                                                              aTGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTgcgc                  <  2:1061253/140‑1 (MQ=255)                                                                                                              aTGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTgcgc                  <  2:193683/140‑1 (MQ=255)                                                                                                              aTGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTgcgc                  <  1:75864/140‑1 (MQ=255)                                                                                                               tGGGCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCt                 >  1:1151531/1‑140 (MQ=255)                                                                                                                 ggCAATTCGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTAt               >  2:729100/1‑140 (MQ=255)                                                                                                                        cGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAaca        <  1:903945/140‑1 (MQ=255)                                                                                                                        cGCTGGTGGAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAaca        <  2:792446/140‑1 (MQ=255)                                                                                                                              tggAGCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATc  >  2:19577/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        AGCCCGCGCGATGAATGGCGTTGGCCTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATC  >  NC_000913/464152‑464416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑