BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	92,589	T→C	L393L (TTG→CTG)	ftsI →	peptidoglycan DD‑transpeptidase FtsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	92,589	0	T	C	100.0%	389.7 / NA	104	L393L (TTG→CTG)	ftsI	peptidoglycan DD‑transpeptidase FtsI
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (46/58); total (46/58)

Only 100 of 105 total aligned reads displayed.

GCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGC  >  NC_000913/92455‑92720

gCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGTGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTc                                                                                                                                <  2:415151/140‑1 (MQ=255)

    aTTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGgag                                                                                                                            >  2:467888/1‑140 (MQ=255)

      tAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGaa                                                                                                                          >  2:194860/1‑140 (MQ=255)

        aCCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACg                                                                                                                        <  2:25165/140‑1 (MQ=255)

         cccTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGc                                                                                                                       <  2:399234/140‑1 (MQ=255)

          ccTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCa                                                                                                                      <  2:882437/140‑1 (MQ=255)

          ccTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCa                                                                                                                      <  1:117189/140‑1 (MQ=255)

          ccTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGACTGGTCGGAGAACGCa                                                                                                                      <  1:1002118/140‑1 (MQ=255)

           cTGTCCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAg                                                                                                                     <  1:77529/140‑1 (MQ=255)

             gACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTg                                                                                                                   >  1:1277204/1‑140 (MQ=255)

              aCCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACg                                                                                                                        >  2:924482/1‑134 (MQ=255)

              aCCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACg                                                                                                                        <  1:924482/134‑1 (MQ=255)

              aCCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTgg                                                                                                                  >  1:1230874/1‑140 (MQ=255)

              aCCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTgg                                                                                                                  >  1:1059482/1‑140 (MQ=255)

                   ggTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGACGGAGAACGCAGTGGCTtat                                                                                                             >  2:950711/1‑140 (MQ=255)

                        tACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCa                                                                                                                      >  1:1142012/1‑126 (MQ=255)

                        tACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGCAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCa                                                                                                                      <  2:1142012/126‑1 (MQ=255)

                          cAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCa                                                                                                      >  1:560910/1‑140 (MQ=255)

                          cAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCa                                                                                                      <  1:316986/140‑1 (MQ=255)

                             aaGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCaaaa                                                                                                   >  1:562558/1‑140 (MQ=255)

                             aaGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCaaaa                                                                                                   >  1:528913/1‑140 (MQ=255)

                             aaGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCaaaa                                                                                                   >  2:1038512/1‑140 (MQ=255)

                             aaGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCaaaa                                                                                                   >  1:281283/1‑140 (MQ=255)

                             aaGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCaaaa                                                                                                   >  2:474146/1‑140 (MQ=255)

                               gTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAaac                                                                                                 >  2:597844/1‑140 (MQ=255)

                                  gAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGATAACGCAGTGGCTTATATCCTCAAAaacaac                                                                                              >  2:894882/1‑140 (MQ=255)

                                  gAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAaacaac                                                                                              >  1:630097/1‑140 (MQ=255)

                                  gAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAaacaac                                                                                              >  1:1225809/1‑140 (MQ=255)

                                    gTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACgg                                                                                            <  1:396303/140‑1 (MQ=255)

                                    gTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACgg                                                                                            <  2:1230874/140‑1 (MQ=255)

                                    gTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACgg                                                                                            <  1:447180/140‑1 (MQ=255)

                                    gTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACgg                                                                                            <  2:853939/140‑1 (MQ=255)

                                    gTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACgg                                                                                            <  2:216797/140‑1 (MQ=255)

                                      aaCGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACggtg                                                                                          <  1:950711/140‑1 (MQ=255)

                                      aaCGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACggtg                                                                                          <  2:777633/140‑1 (MQ=255)

                                      aaCGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACggtg                                                                                          <  1:1259759/140‑1 (MQ=255)

                                      aaCGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACggtg                                                                                          <  1:194860/140‑1 (MQ=255)

                                        cGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACggtggt                                                                                        <  2:387835/140‑1 (MQ=255)

                                        cGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACggtggt                                                                                        <  2:7410/140‑1 (MQ=255)

                                        cGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACggtggt                                                                                        <  2:221320/140‑1 (MQ=255)

                                        cGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACggtggt                                                                                        <  1:989362/140‑1 (MQ=255)

                                         gTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTc                                                                                       >  1:169371/1‑140 (MQ=255)

                                             gtgtTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGAc                                                                                   >  2:25715/1‑140 (MQ=255)

                                                     aaGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATagagag                                                                           >  1:534710/1‑140 (MQ=255)

                                                      aGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAgg                                                                          <  2:1059482/140‑1 (MQ=255)

                                                          ggCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCa                                                                      >  2:310526/1‑140 (MQ=255)

                                                            cGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCAcc                                                                    >  1:231267/1‑140 (MQ=255)

                                                            cGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCAcc                                                                    >  2:84223/1‑140 (MQ=255)

                                                             gTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCt                                                                   <  2:177091/140‑1 (MQ=255)

                                                             gTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCt                                                                   <  2:118856/140‑1 (MQ=255)

                                                                  cGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTctct                                                              >  2:701203/1‑140 (MQ=255)

                                                                          tCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTa                                                      <  1:1248972/140‑1 (MQ=255)

                                                                          tCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTa                                                      >  1:550438/1‑140 (MQ=255)

                                                                            cTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACg                                                    >  1:749979/1‑140 (MQ=255)

                                                                              cAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACggg                                                  <  2:534710/140‑1 (MQ=255)

                                                                              cAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACggg                                                  <  2:528913/140‑1 (MQ=255)

                                                                                gCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCt                                                <  1:54194/140‑1 (MQ=255)

                                                                                gCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCt                                                <  1:726520/140‑1 (MQ=255)

                                                                                gCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCt                                                <  2:630097/140‑1 (MQ=255)

                                                                                gCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCt                                                <  1:474146/140‑1 (MQ=255)

                                                                                 cGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTa                                               <  1:467888/140‑1 (MQ=255)

                                                                                    tagtagATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTaa                                              >  2:468349/1‑138 (MQ=255)

                                                                                    tagtagATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTaa                                              <  1:468349/138‑1 (MQ=255)

                                                                                      gtagATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGt                                          <  1:439902/140‑1 (MQ=255)

                                                                                      gtagATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGt                                          <  1:563243/140‑1 (MQ=255)

                                                                                      gtagATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGt                                          <  1:84223/140‑1 (MQ=255)

                                                                                       tagATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTa                                         >  2:946460/1‑140 (MQ=255)

                                                                                        agATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTaa                                        <  1:700733/140‑1 (MQ=255)

                                                                                         gATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACg                                                    >  1:824506/1‑127 (MQ=255)

                                                                                         gATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACg                                                    <  2:824506/127‑1 (MQ=255)

                                                                                          aTACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCGCCTTCTCTTTCGGCTACGGGCTAATGGTAaca                                      <  1:894882/140‑1 (MQ=255)

                                                                                          aTACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAaca                                      <  2:968047/140‑1 (MQ=255)

                                                                                          aTACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAaca                                      <  1:1038512/140‑1 (MQ=255)

                                                                                             cttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCt                                                <  2:253626/127‑1 (MQ=255)

                                                                                             cttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCt                                                >  1:253626/1‑127 (MQ=255)

                                                                                              ttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCt                                                <  2:700763/126‑1 (MQ=255)

                                                                                              ttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCAt                                  >  1:1144348/1‑140 (MQ=255)

                                                                                              ttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCAt                                  <  1:259597/140‑1 (MQ=255)

                                                                                                actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCTCCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTa                                <  2:690908/140‑1 (MQ=255)

                                                                                                actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCt                                                >  1:80060/1‑124 (MQ=255)

                                                                                                actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCt                                                <  2:80060/124‑1 (MQ=255)

                                                                                                actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTaa                                        <  1:1260073/132‑1 (MQ=255)

                                                                                                actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTaa                                        >  2:1260073/1‑132 (MQ=255)

                                                                                                actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTa                                >  1:690908/1‑140 (MQ=255)

                                                                                                actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTa                                >  2:355108/1‑140 (MQ=255)

                                                                                                actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTa                                >  2:234303/1‑140 (MQ=255)

                                                                                                actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTa                                >  2:224395/1‑140 (MQ=255)

                                                                                                actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTa                                >  2:182834/1‑140 (MQ=255)

                                                                                                   cACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAg                             >  1:1110259/1‑140 (MQ=255)

                                                                                                       ttttGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAg                         <  2:1277204/140‑1 (MQ=255)

                                                                                                         ttGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAaca                                      <  2:726882/125‑1 (MQ=255)

                                                                                                         ttGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAaca                                      >  1:726882/1‑125 (MQ=255)

                                                                                                         ttGGACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAgcg                       <  1:182834/140‑1 (MQ=255)

                                                                                                            gACTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGa                    <  1:597844/140‑1 (MQ=255)

                                                                                                              cTGGGAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGt                  <  1:701203/140‑1 (MQ=255)

                                                                                                                 ggAAAAGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTa               >  2:850440/1‑140 (MQ=255)

                                                                                                                   aaaaGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACg             <  2:550438/140‑1 (MQ=255)

                                                                                                                      aGCGACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCaa          >  1:152987/1‑140 (MQ=255)

                                                                                                                         gACCAATTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTa       <  2:152987/140‑1 (MQ=255)

                                                                                                                              aTTTGGGGCTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGc  <  1:224395/140‑1 (MQ=255)

GCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGC  >  NC_000913/92455‑92720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`