Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	549,638	C→T	I4I (ATC→ATT)	ylbF →	DUF2877 domain‑containing protein YlbF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	549,638	0	C	T	100.0%	179.9 / NA	50	I4I (ATC→ATT)	ylbF	DUF2877 domain‑containing protein YlbF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/26);  total (24/26)

GTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAG  >  NC_000913/549501‑549766                                                                                                                                          |                                                                                                                                 gTATCGCCCATAAAGAGGCGGGGGTCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGGGCTTTTAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGGATTGGTTGAGGAACGCGCAAAGACGATcattca                                                                                                                                >  2:1020163/1‑140 (MQ=255) gTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATcattca                                                                                                                                >  1:434081/1‑140 (MQ=255)   aTCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATcattcatc                                                                                                                              <  2:741126/140‑1 (MQ=255)     cGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCct                                                                                                                            <  1:769514/140‑1 (MQ=255)      gCCCATAAAGAGGCGGGGGTCGGGGAGATTTGGGCCGGCCCCCGGCGGGGACCCCCGGCGGGGTTTTAAAAGGCGCCGGAAGCACCGGCTGAAAACAAGGGGATTTGTTGAGGAAAGCGCAAAGACGAACattcatcccc                                                                                                                           >  2:633437/1‑138 (MQ=255)      gCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCctc                                                                                                                           >  2:816451/1‑140 (MQ=255)       cccATAAAGAGGCGGGGATCGGGGAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGGGCTTTTAACAGGCGCTGGGAGCACTGGCTGAAAGCATGGGGATTGGGTGAGGAACGCGCAATGACGATCattcatccctg                                                                                                                          >  2:240442/1‑137 (MQ=255)         cATAAAGAGGCGGGGGTCGGGCAGAATTGCGCAGGCACCGTGCGGGGACCGCCGGCGTGCTTTGAACAAGCGCTGGGAACACTGGGTGAAAACATGGGGATTTGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGc                                                                                                                        >  2:578617/1‑140 (MQ=255)           tAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCtt                                                                                                                      <  1:557217/140‑1 (MQ=255)            aaaGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTg                                                                                                                     <  1:816451/140‑1 (MQ=255)             aaGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGc                                                                                                                    <  1:185711/140‑1 (MQ=255)             aaGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGc                                                                                                                    <  2:434081/140‑1 (MQ=255)              agagGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGcc                                                                                                                   <  1:1068840/140‑1 (MQ=255)                  gCGGGGGTCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGGAAGCATGGGGATTTGTTGAGGGACGCGCAATGACGATCATTCATCCTCTGCTTGCCcgta                                                                                                               >  2:1068840/1‑140 (MQ=255)                        aTCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAgcg                                                                                                         <  1:1129724/140‑1 (MQ=255)                            ggCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCAcc                                                                                                     >  1:1241917/1‑140 (MQ=255)                                gATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAAt                                                                                                 >  1:1101543/1‑140 (MQ=255)                                gATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAAt                                                                                                 <  1:633437/140‑1 (MQ=255)                                       gcAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTACTTGCCAGTAGTAGCGCACCGAATTATCGcc                                                                                          <  2:247453/140‑1 (MQ=255)                                               cGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTgg                                                                                  <  2:32553/140‑1 (MQ=255)                                               cGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTgg                                                                                  <  2:1101543/140‑1 (MQ=255)                                               cGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTgg                                                                                  >  1:1187827/1‑140 (MQ=255)                                                 tGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCg                                                                                <  2:1241917/140‑1 (MQ=255)                                                     ggCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTa                                                                            >  1:1027861/1‑140 (MQ=255)                                                     ggCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTa                                                                            >  1:805483/1‑140 (MQ=255)                                                      gCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAg                                                                           >  2:890482/1‑140 (MQ=255)                                                        aCCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGc                                                                                           >  1:733908/1‑122 (MQ=255)                                                        aCCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGc                                                                                           <  2:733908/122‑1 (MQ=255)                                                            cTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCgg                                                                        >  1:185700/1‑137 (MQ=255)                                                            cTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCgg                                                                        <  2:185700/137‑1 (MQ=255)                                                                    cTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTggcgg                                                             >  1:430112/1‑140 (MQ=255)                                                                      ttGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGgcggcg                                                           <  1:890482/140‑1 (MQ=255)                                                                      ttGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGgcggcg                                                           <  1:309655/140‑1 (MQ=255)                                                                              gcgcTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTa                                                   >  1:200883/1‑140 (MQ=255)                                                                                   ggAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCtg                                              >  1:456/1‑140 (MQ=255)                                                                                        cACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGAc                                         >  2:66946/1‑140 (MQ=255)                                                                                                gAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAgcg                                 <  1:230785/140‑1 (MQ=255)                                                                                                   aGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAgcggcg                              <  1:922002/140‑1 (MQ=255)                                                                                                   aGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAgcggcg                              <  2:1036171/140‑1 (MQ=255)                                                                                                    gCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGa                             <  1:190313/140‑1 (MQ=255)                                                                                                       tGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGGGGTTAACCTGATGGAGGAAAGCGGCGGACt                          >  2:922002/1‑140 (MQ=255)                                                                                                       tGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGGTTAACCTGATGACGGGAAGCGGCGGACt                          >  1:621204/1‑140 (MQ=255)                                                                                                       tGGGTATTGGTTGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACt                          >  1:121900/1‑140 (MQ=255)                                                                                                                 ttGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTg                <  1:1020163/140‑1 (MQ=255)                                                                                                                  tGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGc               <  1:578617/140‑1 (MQ=255)                                                                                                                  tGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGc               <  1:66946/140‑1 (MQ=255)                                                                                                                  tGAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGc               <  2:200883/140‑1 (MQ=255)                                                                                                                   gAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTTACCTGATGACGGAAAGCGGCGAAATGTTAACGTTGCa              >  2:594963/1‑140 (MQ=255)                                                                                                                   gAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCa              >  2:431260/1‑140 (MQ=255)                                                                                                                   gAGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAACGGCGAACTGTTAACGTTGCa              >  1:946857/1‑140 (MQ=255)                                                                                                                    aGGAACGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCAt             >  1:1104282/1‑140 (MQ=255)                                                                                                                        aCGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTc         >  1:148850/1‑140 (MQ=255)                                                                                                                        aCGCGCAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTc         >  1:1083051/1‑140 (MQ=255)                                                                                                                          gcgcAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCCTCGTCAg       >  1:541981/1‑140 (MQ=255)                                                                                                                           cgcAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGGGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGGAAGCGGCGAACTGTTAACGTTGCATCGGCAgg      >  2:1189838/1‑140 (MQ=255)                                                                                                                           cgcAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGt          <  2:221878/136‑1 (MQ=255)                                                                                                                           cgcAATGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGt          >  1:221878/1‑136 (MQ=255)                                                                                                                               aTGACGATCATTCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAg  <  2:1027861/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 GTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAG  >  NC_000913/549501‑549766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑