Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	634,857	C→T	G174S (GGC→AGC)	ybdM ←	ParB‑like nuclease domain‑containing protein YbdM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	634,857	0	C	T	100.0%	176.1 / NA	49	G174S (GGC→AGC)	ybdM	ParB‑like nuclease domain‑containing protein YbdM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/23);  total (26/23)

AGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACA  >  NC_000913/634722‑634992                                                                                                                                        |                                                                                                                                        agcTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAAtt                                                                                                                                     >  1:839819/1‑140 (MQ=255) agcTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAAtt                                                                                                                                     <  1:1261491/140‑1 (MQ=255)             gcgcCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGtcc                                                                                                                         >  1:461990/1‑140 (MQ=255)              cgcCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGtccc                                                                                                                        >  2:1063875/1‑140 (MQ=255)                     tttAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCaa                                                                                                                 <  2:452421/140‑1 (MQ=255)                         gCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTATTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTgg                                                                                                             <  2:955329/140‑1 (MQ=255)                           tATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCt                                                                                                           <  1:114212/140‑1 (MQ=255)                                     tCCAGGCGCGGGAATATTGTCGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACgg                                                                                                 >  2:1117684/1‑140 (MQ=255)                                     tCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACgg                                                                                                 >  2:473295/1‑140 (MQ=255)                                      ccAGGCGCGGGAATATTGTCGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACAGa                                                                                                <  1:1117684/140‑1 (MQ=255)                                      ccAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGa                                                                                                <  2:839819/140‑1 (MQ=255)                                       cAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGAc                                                                                               >  2:84844/1‑140 (MQ=255)                                               ggAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCg                                                                                       >  2:895156/1‑140 (MQ=255)                                               ggAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCg                                                                                       >  2:878178/1‑140 (MQ=255)                                               ggAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCg                                                                                       >  2:640700/1‑140 (MQ=255)                                                 aaTATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGAc                                                                                     <  1:276928/140‑1 (MQ=255)                                                        aCGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCgg                                                                              <  1:1170428/140‑1 (MQ=255)                                                            tCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGAt                                                                          >  2:131460/1‑140 (MQ=255)                                                            tCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGAt                                                                          >  1:1022205/1‑140 (MQ=255)                                                               gCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTg                                                                       <  1:84844/140‑1 (MQ=255)                                                                         ctTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCa                                                             <  2:461990/140‑1 (MQ=255)                                                                                 ccGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGcgcg                                                     >  2:1035872/1‑140 (MQ=255)                                                                                    ttAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTTATGGCGTCCACGAGCGCGAtt                                                  >  2:828018/1‑140 (MQ=255)                                                                                            cTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAgc                                                        <  1:1117249/126‑1 (MQ=255)                                                                                            cTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAgc                                                        >  2:1117249/1‑126 (MQ=255)                                                                                               cAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGGCCACGAGCGCGATTATGACGAATaa                                       >  2:251506/1‑139 (MQ=255)                                                                                                                 gCTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGAt                     <  1:878178/140‑1 (MQ=255)                                                                                                                  cTGTCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGAtt                    >  2:980641/1‑140 (MQ=255)                                                                                                                     tCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTAcg                 >  2:169258/1‑140 (MQ=255)                                                                                                                     tCCATTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTAcg                 >  2:411202/1‑140 (MQ=255)                                                                                                                        aTTCCCAGCTCTTTGCTAATTTTATTGTCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  1:895156/140‑1 (MQ=255)                                                                                                                        aTTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  1:131460/140‑1 (MQ=255)                                                                                                                        aTTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  1:169258/140‑1 (MQ=255)                                                                                                                        aTTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  1:473295/140‑1 (MQ=255)                                                                                                                        aTTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  1:640700/140‑1 (MQ=255)                                                                                                                        aTTCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  1:827884/140‑1 (MQ=255)                                                                                                                          tCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTTATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGGTGCGTTGATTACGCGTAc            >  2:865191/1‑140 (MQ=255)                                                                                                                          tCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTTAAGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGGTGGTTAGGCGTAc            >  2:284422/1‑140 (MQ=255)                                                                                                                          tCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              >  1:807672/1‑138 (MQ=255)                                                                                                                          tCCCAGCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  2:807672/138‑1 (MQ=255)                                                                                                                               gCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTAc                  <  2:544227/129‑1 (MQ=255)                                                                                                                               gCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTAc                  >  1:544227/1‑129 (MQ=255)                                                                                                                               gCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTcc       >  1:689981/1‑140 (MQ=255)                                                                                                                               gCTCTTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTcc       >  1:358671/1‑140 (MQ=255)                                                                                                                                ctctTTGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCATCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCa      <  2:1022205/140‑1 (MQ=255)                                                                                                                                    ttGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  1:881667/128‑1 (MQ=255)                                                                                                                                    ttGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              >  2:881667/1‑128 (MQ=255)                                                                                                                                    ttGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACa  >  1:1038087/1‑140 (MQ=255)                                                                                                                                     tGCTAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGt              <  2:736210/127‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        AGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCCATCCCAACTGGCTAAGTTCACGGACGATTTCCGACATGGCGGTGATTTGATGGCGTCCACGAGCGCGATTATGACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACA  >  NC_000913/634722‑634992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑