Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 118,297 C→T G117S (GGC→AGC)  nadC ← quinolinate phosphoribosyltransferase (decarboxylating)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913118,2970CT98.4% 220.6 / NA 63G117S (GGC→AGC) nadCquinolinate phosphoribosyltransferase (decarboxylating)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (34/28);  minor base G (0/1);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGC  >  NC_000913/118160‑118431
                                                                                                                                         |                                                                                                                                      
agCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCg                                                                                                                                      >  2:545617/1‑140 (MQ=255)
     aaCGCAGGCCGGGTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAAc                                                                                                                                 >  2:354864/1‑140 (MQ=255)
     aaCGCAGGCCGGGTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAAc                                                                                                                                 >  2:370583/1‑140 (MQ=255)
     aaCGCAGGCCGGGTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAAc                                                                                                                                 >  1:377084/1‑140 (MQ=255)
       cGCAGGCCGGGTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAg                                                                                                                               <  1:1187672/140‑1 (MQ=255)
         cAGGCCGGGTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCa                                                                                                                             <  1:137649/140‑1 (MQ=255)
           ggCCGGGTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCAcg                                                                                                                           <  1:94407/140‑1 (MQ=255)
           ggCCGGGTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCAcg                                                                                                                           <  1:545617/140‑1 (MQ=255)
               gggTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCggg                                                                                                                       >  1:1262558/1‑140 (MQ=255)
               gggTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCggg                                                                                                                       >  1:624660/1‑140 (MQ=255)
                ggTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGa                                                                                                                      >  1:822646/1‑140 (MQ=255)
                          tGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCa                                                                                                            >  1:204135/1‑140 (MQ=255)
                          tGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCa                                                                                                            >  1:25528/1‑140 (MQ=255)
                           gcgcGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTc                                                                                                             >  1:933923/1‑138 (MQ=255)
                           gcgcGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTc                                                                                                             <  2:933923/138‑1 (MQ=255)
                              cgTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGtt                                                                                                        <  2:481988/140‑1 (MQ=255)
                              cgTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCGCGTTAACAGCACGCGGGATGGGCCTTCAAGtt                                                                                                        <  1:934850/140‑1 (MQ=255)
                                 aTCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGa                                                                                                     >  2:751272/1‑140 (MQ=255)
                                        aacTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGga                                                                                              <  2:344661/140‑1 (MQ=255)
                                             cGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGgattgat                                                                                         >  1:538718/1‑140 (MQ=255)
                                             cGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGgattgat                                                                                         >  2:413031/1‑140 (MQ=255)
                                             cGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGgattgat                                                                                         >  1:893177/1‑140 (MQ=255)
                                             cGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGgattgat                                                                                         <  1:370583/140‑1 (MQ=255)
                                             cGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGgattgat                                                                                         <  2:564238/140‑1 (MQ=255)
                                                 ttGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGc                                                                                     <  1:751272/140‑1 (MQ=255)
                                                     tGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATtg                                                                                 >  1:882853/1‑140 (MQ=255)
                                                      gCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATtga                                                                                <  2:538718/140‑1 (MQ=255)
                                                       ccTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATtgat                                                                               >  1:1171512/1‑140 (MQ=255)
                                                           ccAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACa                                                                           <  2:121400/140‑1 (MQ=255)
                                                              gCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCg                                                                        <  2:452126/140‑1 (MQ=255)
                                                              gCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCg                                                                        <  2:25528/140‑1 (MQ=255)
                                                              gCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCg                                                                        <  2:1157354/140‑1 (MQ=255)
                                                                aaTTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGcc                                                                      <  2:951118/140‑1 (MQ=255)
                                                                  ttCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGTATTGATGACATCGCCGt                                                                    >  2:953977/1‑140 (MQ=255)
                                                                      aCATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATc                                                                <  1:413031/140‑1 (MQ=255)
                                                                       cATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATcc                                                               >  1:477263/1‑140 (MQ=255)
                                                                        aTAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCca                                                              <  2:477263/140‑1 (MQ=255)
                                                                        aTAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCca                                                              <  2:204135/140‑1 (MQ=255)
                                                                         tAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCcac                                                             <  1:137012/140‑1 (MQ=255)
                                                                                 tACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGa                                                     >  2:982291/1‑140 (MQ=255)
                                                                                    cTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTa                                                  >  2:245177/1‑140 (MQ=255)
                                                                                    cTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTa                                                  >  2:320149/1‑140 (MQ=255)
                                                                                    cTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTa                                                  >  2:510446/1‑140 (MQ=255)
                                                                                     ttACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGc                                                         <  2:886677/132‑1 (MQ=255)
                                                                                     ttACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGc                                                         >  1:886677/1‑132 (MQ=255)
                                                                                             aaCTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAt                                         >  1:776213/1‑140 (MQ=255)
                                                                                              aCTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtc                                        >  2:484080/1‑140 (MQ=255)
                                                                                              aCTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtc                                        >  2:519291/1‑140 (MQ=255)
                                                                                                tCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACAtcgt                                      >  1:806219/1‑140 (MQ=255)
                                                                                                       aGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGTATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGc                               <  1:953977/140‑1 (MQ=255)
                                                                                                       aGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGc                               <  2:110334/140‑1 (MQ=255)
                                                                                                          gTTTGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAg                            >  1:234626/1‑140 (MQ=255)
                                                                                                            ttGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGtt                          >  1:922042/1‑140 (MQ=255)
                                                                                                            ttGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGtt                          >  1:753887/1‑140 (MQ=255)
                                                                                                             tGCACAAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTg                         <  1:982291/140‑1 (MQ=255)
                                                                                                                 caAAATTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATa                     <  2:1262558/140‑1 (MQ=255)
                                                                                                                   aaaTTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATaaa                   >  1:923067/1‑140 (MQ=255)
                                                                                                                    aaTTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAac                  <  2:83168/140‑1 (MQ=255)
                                                                                                                    aaTTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAac                  >  1:550858/1‑140 (MQ=255)
                                                                                                                    aaTTAAGCGCAGTGCGTTCGCTCGTTAACAGCACGAGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAac                  <  2:882853/140‑1 (MQ=255)
                                                                                                                      ttAAGCGCAGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAacac                <  2:806219/140‑1 (MQ=255)
                                                                                                                              aGTGCGTTCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCaa        >  2:202093/1‑140 (MQ=255)
                                                                                                                                    ttCGCTCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGc  >  2:767545/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                      
AGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCACGCGGGATGGGCCTTCAAGTTCGAACAAGGATTGATTGGCATTGATGACATCGCCGTCATCCACATGCCAGATTATGGTGACATCGTCGCCTGCCAGTTGAATAAACACCTCTTCAACCCAGC  >  NC_000913/118160‑118431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑