Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,090,516	C→T	E592K (GAG→AAG)	pgaA ←	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine outer membrane porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,090,516	0	C	T	100.0%	104.5 / NA	30	E592K (GAG→AAG)	pgaA	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine outer membrane porin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/10);  total (20/10)

TGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAAT  >  NC_000913/1090379‑1090650                                                                                                                                          |                                                                                                                                       tGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGa                                                                                                                                      >  2:211949/1‑140 (MQ=255)   cccGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGc                                                                                                                                    <  2:976311/140‑1 (MQ=255)   cccGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGc                                                                                                                                    <  2:946095/140‑1 (MQ=255)   cccGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGc                                                                                                                                    <  1:211949/140‑1 (MQ=255)    ccGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGcc                                                                                                                                   >  2:630099/1‑140 (MQ=255)    ccGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGcc                                                                                                                                   <  1:423107/140‑1 (MQ=255)     cGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCa                                                                                                                                  <  2:681456/140‑1 (MQ=255)     cGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCa                                                                                                                                  <  2:478691/140‑1 (MQ=255)     cGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCa                                                                                                                                  <  2:278180/140‑1 (MQ=255)       tAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGACTGAAAACGCGTTCAGCGTCCTCTGCCTCGAGCCAGa                                                                                                                                <  2:224840/140‑1 (MQ=255)        aaTGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGat                                                                                                                               >  1:1221145/1‑140 (MQ=255)             aaCGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTAc                                                                                                                          <  2:684041/140‑1 (MQ=255)                    tGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCg                                                                                                                   >  1:278083/1‑140 (MQ=255)                            gCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCaa                                                                                                           >  2:863637/1‑140 (MQ=255)                            gCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCaa                                                                                                           >  1:78388/1‑140 (MQ=255)                                tCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCAGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACAcc                                                                                                       >  1:301605/1‑140 (MQ=255)                                cccAGTGGCAACCAAATCCCCCATTTATCATAAAAATCATACCAGCCAGACAGGCGCGCGCGGGGTTTATGCTAATGATTAAAACCGCGTTCAGCGTCCTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACAcc                                                                                                       <  2:335552/139‑1 (MQ=255)                                       gCGAACCAATCCGCCATTTATAATAAAAATCATACCAGCCAAACGGGCGCGCGCGGGGTTTATGCTCATGATTGAAACCGCGTTCAGCGTCCTCGGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGc                                                                                                <  2:871018/140‑1 (MQ=255)                                             cAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTcg                                                                                          <  1:1136470/140‑1 (MQ=255)                                               aTACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTcacg                                                                                        >  1:784255/1‑140 (MQ=255)                                                  ccccttttATAATAAAAATCACCCCGCCAACACGGGCGGGCGCGGGTTTTATGCTGATGATGAAACCCGCGTGAAGTGTCCTCCCCTTGGAGCCAGTTATTCGGGGACCGCCACTCAACACCCGCAAGCCAGTCGCGAAc                                                                                     <  2:326031/134‑1 (MQ=255)                                                  cGCCATTTATCATAAAAATCATACCAGCCAGACGGGCGCGCGCGGGGTTTATGCTCATGATTGAAACCGCGTTCAGCGTCCTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAAc                                                                                     <  2:78388/140‑1 (MQ=255)                                                   cccAATTATAATAAAAAACATCCCAGCCAGACAGGCGCGCGCGGGTTTTATGCTAATGATTGAAAACCCTTCCACCGTCCTCTGCTTTGACCAAGATATTCCGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACa                                                                                    <  2:250315/139‑1 (MQ=255)                                                       gTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATcc                                                                                >  2:407679/1‑140 (MQ=255)                                                       gTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATcc                                                                                >  2:23991/1‑140 (MQ=255)                                                                      aTACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTaa                                                                 >  2:984581/1‑140 (MQ=255)                                                                       tcccagccagACAGGCGCGCGCGGGTTTTATGCTCATGATTAAAACCGCTTTCAGCGTCCTCTCCCTTGACCCAAATATTCCGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTaaa                                                                <  2:1135724/138‑1 (MQ=255)                                                                        aCCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAAt                                                               >  2:1007472/1‑140 (MQ=255)                                                                                    ggCCCGCCCGGGGTTTATGTTAATAATCGAAAGCGCTTCCAGCAATCTCTGCCTCGCGCCAAATATTACGCGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCa                                                   <  2:961415/140‑1 (MQ=255)                                                                                     gcgcgcgcCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCAt                                                  >  1:1029126/1‑140 (MQ=255)                                                                                      cgccccccGGGTTTAGGCTCATGTTTAAAAACGCTTTCGGCGTCCCCTGCCTTGACCAATATATTCCGGGTCCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTCTCCTTCGCTAAATTGTCCATCGGCATa                                                 <  2:13155/134‑1 (MQ=255)                                                                                        cgcgcCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAAc                                               >  1:116505/1‑140 (MQ=255)                                                                                                 tttATGCTCATGATTAAAAACGCGTTCAGCGTACTCTGCTTTGAGCCAGATATTCCGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAg                                      <  1:216192/140‑1 (MQ=255)                                                                                                         cATGATTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACcg                              >  2:739234/1‑140 (MQ=255)                                                                                                          aTGATTGAAACCGCGTTCAGCGTTCTCTGCCTGGGGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACcgc                             <  1:151054/140‑1 (MQ=255)                                                                                                          aTGATAGAAAACGCGTTCGGCGTACTCCGCCTGGAGCAAGATATTACGGCACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACcgc                             <  2:301605/140‑1 (MQ=255)                                                                                                            gATTAAAACCGCTTCCGGCTTCCTCTCCCTGGACCCAGATATTCCGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCgcgc                           <  1:630099/140‑1 (MQ=255)                                                                                                             tttGAAACCGCGTTCAGCGTCCTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCACGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGcc                          <  2:784255/139‑1 (MQ=255)                                                                                                             aTTGAAAACGCGTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGcc                          <  1:863637/140‑1 (MQ=255)                                                                                                             aTTAAAACCGCGTTCAGCGTACTCTGCCTGGAGCCAGATTTTCCGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGcc                          <  2:116505/140‑1 (MQ=255)                                                                                                                     cgcgTTCAGCGTCCTCTGCCTTGAGCCAGATATTCCGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATcc                  <  1:369427/140‑1 (MQ=255)                                                                                                                      gcgTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACcgc                             <  1:784777/128‑1 (MQ=255)                                                                                                                      gcgTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACcgc                             >  2:784777/1‑128 (MQ=255)                                                                                                                      gcgTTCAGCGTACTCTGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCt                 >  2:1032923/1‑140 (MQ=255)                                                                                                                               gTACTCTGCCTTGACCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCggtg        <  2:1221145/140‑1 (MQ=255)                                                                                                                                   tctGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGa    <  1:23991/140‑1 (MQ=255)                                                                                                                                    ctGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCggtg        >  1:177345/1‑135 (MQ=255)                                                                                                                                    ctGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCggtg        <  2:177345/135‑1 (MQ=255)                                                                                                                                    ctGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGaa   >  1:13422/1‑140 (MQ=255)                                                                                                                                     tGCCTTGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAAt  >  1:159681/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAAT  >  NC_000913/1090379‑1090650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑