Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,185,300	C→T	A99T (GCA→ACA)	potA ←	spermidine preferential ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,185,300	0	C	T	100.0%	278.0 / NA	78	A99T (GCA→ACA)	potA	spermidine preferential ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/41);  total (37/41)

TTGCGTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAACGGTTGTTTTACCGCA  >  NC_000913/1185164‑1185435                                                                                                                                         |                                                                                                                                        ttGCGTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAA‑CCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAg                                                                                                                                     >  2:238220/1‑140 (MQ=255)  tGCGTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAg                                                                                                                                     <  1:563491/140‑1 (MQ=255)    cGTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCt                                                                                                                                   <  2:1072593/140‑1 (MQ=255)         aGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGa                                                                                                                              >  1:849867/1‑140 (MQ=255)            gAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGaaaa                                                                                                                           <  1:1160776/140‑1 (MQ=255)             aaGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAAc                                                                                                                          <  2:598972/140‑1 (MQ=255)             aaGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAAc                                                                                                                          <  1:539091/140‑1 (MQ=255)             aaGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAAc                                                                                                                          <  2:309927/140‑1 (MQ=255)              aGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACa                                                                                                                         >  1:1095140/1‑140 (MQ=255)                  ttCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAgtgt                                                                                                                     <  2:11398/140‑1 (MQ=255)                       aCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTcaca                                                                                                                <  2:735663/140‑1 (MQ=255)                          gtaCCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGaaaa                                                                                                                           >  1:1128535/3‑126 (MQ=255)                          gtaCCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGaaaa                                                                                                                           <  2:1128535/124‑1 (MQ=255)                          gCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTca                                                                                                                  <  1:441357/135‑1 (MQ=255)                          gCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTca                                                                                                                  >  2:441357/1‑135 (MQ=255)                          gCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTca                                                                                                                  <  1:535202/135‑1 (MQ=255)                          gCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTca                                                                                                                  >  2:535202/1‑135 (MQ=255)                          gCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAg                                                                                                             >  1:943164/1‑140 (MQ=255)                                tCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGttt                                                                                                       >  1:101267/1‑140 (MQ=255)                                   gCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTcc                                                                                                    >  1:1052070/1‑140 (MQ=255)                                   gCAGGGCCTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTcc                                                                                                    >  1:95870/1‑140 (MQ=255)                                     aGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgc                                                                                                  >  2:620428/1‑140 (MQ=255)                                        gCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCgg                                                                                               >  2:330967/1‑140 (MQ=255)                                          ttCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGaa                                                                                             >  1:273916/1‑140 (MQ=255)                                          ttCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGaa                                                                                             >  1:665946/1‑140 (MQ=255)                                           tCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAAc                                                                                            <  1:595032/140‑1 (MQ=255)                                           tCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAAc                                                                                            >  1:1159684/1‑140 (MQ=255)                                           tCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAAc                                                                                            >  1:22666/1‑140 (MQ=255)                                            ccATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACg                                                                                           >  2:1074080/1‑140 (MQ=255)                                             cATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGt                                                                                          >  2:201196/1‑140 (MQ=255)                                             cATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGt                                                                                          <  2:849867/140‑1 (MQ=255)                                                cACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg                                                                                       <  2:273916/140‑1 (MQ=255)                                                 aCGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGt                                                                                      >  1:683560/1‑140 (MQ=255)                                                  cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTg                                                                                     >  1:450918/1‑140 (MQ=255)                                                  cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTg                                                                                     <  1:193657/140‑1 (MQ=255)                                                  cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTg                                                                                     <  2:101267/140‑1 (MQ=255)                                                  cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTg                                                                                     <  2:450918/140‑1 (MQ=255)                                                      gggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCTCCGGAACGTGGGt                                                                                      <  1:982565/135‑1 (MQ=255)                                                      gggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCTCCGGAACGTGGGt                                                                                      >  2:982565/1‑135 (MQ=255)                                                      gggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGt                                                                                 <  2:432879/140‑1 (MQ=255)                                                      gggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGt                                                                                 <  2:665946/140‑1 (MQ=255)                                                      gggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGt                                                                                 <  1:454659/140‑1 (MQ=255)                                                       ggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTc                                                                                >  2:284746/1‑140 (MQ=255)                                                           tAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCg                                                                            <  2:436974/140‑1 (MQ=255)                                                           tAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCg                                                                            <  1:1128511/140‑1 (MQ=255)                                                           tAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCg                                                                            <  1:620428/140‑1 (MQ=255)                                                                tCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATc                                                                       <  2:683560/140‑1 (MQ=255)                                                                         ggggTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCatgat                                                              <  2:281767/140‑1 (MQ=255)                                                                         ggggTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCatgat                                                              <  2:994213/140‑1 (MQ=255)                                                                         ggggTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCatgat                                                              >  1:734155/1‑140 (MQ=255)                                                                          gggTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCatgatg                                                             <  2:1052070/140‑1 (MQ=255)                                                                               ttttGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTcc                                                        <  1:891711/140‑1 (MQ=255)                                                                                       gcgcAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCaa                                                <  1:160213/140‑1 (MQ=255)                                                                                             cccAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGttt                                          >  2:1205735/1‑140 (MQ=255)                                                                                              ccAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTc                                         <  1:1205735/140‑1 (MQ=255)                                                                                                aaaGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCa                                       <  2:943164/140‑1 (MQ=255)                                                                                                       acaTTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGc                                >  1:782930/1‑140 (MQ=255)                                                                                                        caTTTTCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCa                               >  2:929574/1‑140 (MQ=255)                                                                                                          ttttCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAAt                             <  1:1030853/140‑1 (MQ=255)                                                                                                          ttttCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAAt                             <  1:1074080/140‑1 (MQ=255)                                                                                                          ttttCGAACACGGTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAAt                             <  1:201196/140‑1 (MQ=255)                                                                                                                     ggTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCgaagaa                  >  2:144310/1‑140 (MQ=255)                                                                                                                     ggTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCgaagaa                  >  2:986512/1‑140 (MQ=255)                                                                                                                     ggTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCgaagaa                  >  1:776249/1‑140 (MQ=255)                                                                                                                      gTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAAc                 <  2:782930/140‑1 (MQ=255)                                                                                                                      gTCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAAc                 <  1:9555/140‑1 (MQ=255)                                                                                                                       tCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAACg                >  2:422295/1‑140 (MQ=255)                                                                                                                       tCATGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAACg                >  2:158009/1‑140 (MQ=255)                                                                                                                         aTGTGGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAACGgt              <  1:929574/140‑1 (MQ=255)                                                                                                                          tgtgGGGGAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAACGgtt             >  2:1243611/1‑140 (MQ=255)                                                                                                                             gggggAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAACGgttgtt          <  2:734155/140‑1 (MQ=255)                                                                                                                              ggggAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAACGGTTGttt         <  1:144310/140‑1 (MQ=255)                                                                                                                               gggAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAACGGTTGtttt        >  1:280763/1‑140 (MQ=255)                                                                                                                                ggAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAACGGTTGTTTTa       >  1:684935/1‑140 (MQ=255)                                                                                                                                 gAAAAGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAACGGTTGTTTTAc      >  2:667265/1‑140 (MQ=255)                                                                                                                                  aaaaGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAACGGTTGTTTTAcc     <  1:330967/140‑1 (MQ=255)                                                                                                                                   aaaGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAACGGTTGTTTTACCg    >  2:591453/1‑140 (MQ=255)                                                                                                                                     aGTGTGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAACGGTTGTTTTACCGCa  >  2:308064/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TTGCGTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGATGTCCTCGTTATCCAGCATGATGCGTCCGGAATCAACAGTTTCCAGACCTGCAATCAGGCGAAGAACGGTTGTTTTACCGCA  >  NC_000913/1185164‑1185435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑