Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,222,450	A→G	V62A (GTT→GCT)	ymgD ←	PF16456 family protein YmgD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,222,450	0	A	G	100.0%	204.4 / NA	54	V62A (GTT→GCT)	ymgD	PF16456 family protein YmgD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/30);  total (24/30)

GCCGATAGCACGCATGGTGTCATCAATTGCCGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCC  >  NC_000913/1222314‑1222581                                                                                                                                         |                                                                                                                                    gCCGATAGCACGCATGGTGTCATCAATTGCCGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAgcgc                                                                                                                                  <  1:1227527/140‑1 (MQ=255)      tAGCACGCATGGTGTCATCAATTGCCGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGca                                                                                                                             <  2:230425/140‑1 (MQ=255)      tAGCACGCATGGTGTCATCAATTGCCGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGca                                                                                                                             <  2:218686/140‑1 (MQ=255)      tAGCACGCATGGTGTCATCAATTGCCGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGca                                                                                                                             <  1:337137/140‑1 (MQ=255)       aGCACGCATGGTGTCATCAATTGCCGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGcat                                                                                                                            <  1:106330/140‑1 (MQ=255)         cACGCATGGTGTCATCAATTGCCGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGcatta                                                                                                                          <  1:475880/140‑1 (MQ=255)                      atcaATTGCCGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGcaa                                                                                                             >  1:411019/1‑140 (MQ=255)                         aaTTGCCGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGcaacaa                                                                                                          <  2:673412/140‑1 (MQ=255)                           ttGCCGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAAtt                                                                                                        >  1:20051/1‑140 (MQ=255)                            tGCCGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAAttt                                                                                                       >  2:690768/1‑140 (MQ=255)                            tGCCGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAAttt                                                                                                       >  2:231941/1‑140 (MQ=255)                            tGCCGTGATCAACAGCATTTACGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAAttt                                                                                                       >  1:698968/1‑140 (MQ=255)                              ccGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCt                                                                                                     >  1:22658/1‑140 (MQ=255)                              ccGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCt                                                                                                     >  1:851999/1‑140 (MQ=255)                                gTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGc                                                                                                   >  2:732647/1‑140 (MQ=255)                                   aTCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAAc                                                                                                >  1:577588/1‑140 (MQ=255)                                        cAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCtgtg                                                                                           >  2:811206/1‑140 (MQ=255)                                            aTTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCt                                                                                       >  1:976705/1‑140 (MQ=255)                                             tttGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTg                                                                                      >  2:340035/1‑140 (MQ=255)                                             tttGCGGGTCTTTAGCGCAAACATGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTg                                                                                      >  2:553914/1‑140 (MQ=255)                                                      cTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGttttt                                                                             >  2:1096083/1‑140 (MQ=255)                                                          aGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCa                                                                         >  1:955748/1‑140 (MQ=255)                                                            cgcAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCAtt                                                                       <  1:134295/140‑1 (MQ=255)                                                              cAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTc                                                                     >  1:683656/1‑140 (MQ=255)                                                                 aCCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGtt                                                                  <  2:698968/140‑1 (MQ=255)                                                                      aTTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCtt                                                             <  2:851999/140‑1 (MQ=255)                                                                      aTTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCtt                                                             <  1:553914/140‑1 (MQ=255)                                                                      aTTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCtt                                                             <  2:20051/140‑1 (MQ=255)                                                                          aGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTgc                                                         <  1:559960/140‑1 (MQ=255)                                                                          aGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTgc                                                         <  2:616293/140‑1 (MQ=255)                                                                            tttttCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTgcgc                                                       <  2:446360/140‑1 (MQ=255)                                                                             ttttCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTgcg                                                        <  1:962812/138‑1 (MQ=255)                                                                             ttttCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTgcg                                                        >  2:962812/1‑138 (MQ=255)                                                                             ttttCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCa                                                      <  2:683656/140‑1 (MQ=255)                                                                             ttttCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCa                                                      <  2:976705/140‑1 (MQ=255)                                                                                   aCCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAat                                                <  2:205522/140‑1 (MQ=255)                                                                                          tGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGtt                                         >  1:40011/1‑140 (MQ=255)                                                                                                  aGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACgg                                 >  1:44976/1‑140 (MQ=255)                                                                                                    ttCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCa                               >  1:43657/1‑140 (MQ=255)                                                                                                    ttCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCa                               <  1:647752/140‑1 (MQ=255)                                                                                                        ggAGATTGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAAcc                           <  1:231941/140‑1 (MQ=255)                                                                                                              tGCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGttttt                     >  2:169407/1‑140 (MQ=255)                                                                                                               gCTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTa                    <  1:340035/140‑1 (MQ=255)                                                                                                                cTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTaa                   <  1:186199/140‑1 (MQ=255)                                                                                                                cTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTaa                   >  1:16659/1‑140 (MQ=255)                                                                                                                cTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTaa                   <  2:16659/140‑1 (MQ=255)                                                                                                                cTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTaa                   >  2:158294/1‑140 (MQ=255)                                                                                                                cTCAATTTTTAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTaa                   <  1:690768/140‑1 (MQ=255)                                                                                                                      ttttAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGt             <  1:1096083/140‑1 (MQ=255)                                                                                                                       tttAAATCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTa            <  2:22658/140‑1 (MQ=255)                                                                                                                            aTCACGGCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCt       <  1:917574/140‑1 (MQ=255)                                                                                                                                 ggCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGcc  <  2:711624/140‑1 (MQ=255)                                                                                                                                 ggCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGcc  <  1:822695/140‑1 (MQ=255)                                                                                                                                 ggCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGcc  >  1:610380/1‑140 (MQ=255)                                                                                                                                 ggCTGGCAGCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGcc  <  1:158294/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    GCCGATAGCACGCATGGTGTCATCAATTGCCGTGATCAACAGCATTTGCGGGTCTTTAGCGCAAACCTGATTCAGTTTTTCTACCACTTTGGCGCTCAGTTCCGGAGATTGCTCAATTTTTAAATCACGGCTGGCAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCC  >  NC_000913/1222314‑1222581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑