Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	133,532	A→G	T640A (ACC→GCC)	acnB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	133,532	0	A	G	100.0%	288.9 / NA	78	T640A (ACC→GCC)	acnB	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/36);  total (42/36)

ACCGATGCGTCCGCCGAGCGTTCTGCCGCTGGTTGTACCATCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTACCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTAAAGAGCCAATCCTGTGTGC  >  NC_000913/133400‑133665                                                                                                                                     |                                                                                                                                      aCCGATGCGTCCGCCGAGCGTTCTGCCGCTGGTTGTACCATCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGa                                                                                                                                <  2:1012819/140‑1 (MQ=255) aCCGATGCGTCCGCCGAGCGTTCTGCCGCTGGTTGTACCATCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGa                                                                                                                                <  1:492506/140‑1 (MQ=255)     aTGCGTCCGCCGAGCGTTCTGCCGCTGGTTGTACCATCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAAcgt                                                                                                                            <  2:484846/140‑1 (MQ=255)     aTGCGTCCGCCGAGCGTTCTGCCGCTGGTTGTACCATCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAAcgt                                                                                                                            <  1:1149429/140‑1 (MQ=255)           ccgccgAGCGTTCTGCCGCTGGTTGTACCATCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTAtt                                                                                                                      >  2:1183145/1‑140 (MQ=255)            cgccgAGCGTTCTGCCGCTGGTTGTACCATCAAGCTGAACAAGGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTc                                                                                                                     >  2:1156051/1‑140 (MQ=255)              ccgAGCGTTCTGCCGCTGGTTGTACCATCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAg                                                                                                                   >  1:638733/1‑140 (MQ=255)                gAGCGTTCTGCCGCTGGTTGTACCATCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAggg                                                                                                                 >  1:1137574/1‑140 (MQ=255)                  gCGTTCTGCCGCTGGTTGTACCATCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCa                                                                                                               <  1:453922/140‑1 (MQ=255)                     ttCTGCCGCTGGTTGTACCATCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATgg                                                                                                            >  1:770829/1‑140 (MQ=255)                             cTGGTTGTACCATCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAAtgg                                                                                                    <  2:658760/140‑1 (MQ=255)                                gTTGTACCATCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAAtggctg                                                                                                 >  2:1213181/1‑140 (MQ=255)                                  tGTACCATCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAAtggctggc                                                                                               <  1:351063/140‑1 (MQ=255)                                         tCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCt                                                                                        >  2:1181201/1‑140 (MQ=255)                                         tCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCt                                                                                        >  2:545890/1‑140 (MQ=255)                                          cAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTg                                                                                       >  1:1277322/1‑140 (MQ=255)                                          cAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTg                                                                                       >  2:17416/1‑140 (MQ=255)                                          cAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTg                                                                                       >  2:565453/1‑140 (MQ=255)                                          cAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCATCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTg                                                                                       >  1:667563/1‑140 (MQ=255)                                            aGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAg                                                                                     <  2:820368/140‑1 (MQ=255)                                                 aaCAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAgctgct                                                                                >  2:205376/1‑140 (MQ=255)                                                             atcatcGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGc                                                                    >  2:824673/1‑140 (MQ=255)                                                               catcGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAg                                                                  <  1:17416/140‑1 (MQ=255)                                                                atcGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGa                                                                 >  2:1022782/1‑140 (MQ=255)                                                                    aaTACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCg                                                             <  1:545890/140‑1 (MQ=255)                                                                    aaTACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCg                                                             <  1:1181201/140‑1 (MQ=255)                                                                           gAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATg                                                               <  1:1250655/131‑1 (MQ=255)                                                                           gAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATg                                                               >  2:1250655/1‑131 (MQ=255)                                                                           gAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATAcg                                                      >  1:902540/1‑140 (MQ=255)                                                                           gAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATAcg                                                      >  1:54260/1‑140 (MQ=255)                                                                            aaCTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATAcgc                                                     >  1:198972/1‑140 (MQ=255)                                                                            aaCTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATAcgc                                                     >  2:177588/1‑140 (MQ=255)                                                                                ctAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCa                                                 <  1:479195/140‑1 (MQ=255)                                                                                ctAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCa                                                 <  1:1183145/140‑1 (MQ=255)                                                                                 tAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAg                                                >  1:133702/1‑140 (MQ=255)                                                                                   aCATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTg                                              >  2:43820/1‑140 (MQ=255)                                                                                   aCATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTg                                              >  2:1035680/1‑140 (MQ=255)                                                                                     aTCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGAt                                            <  2:198972/140‑1 (MQ=255)                                                                                      tCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATc                                           <  1:1035680/140‑1 (MQ=255)                                                                                        gTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATc                                           >  1:1124054/1‑138 (MQ=255)                                                                                        gTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATc                                           <  2:1124054/138‑1 (MQ=255)                                                                                         tCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGAc                                        >  2:867167/1‑140 (MQ=255)                                                                                         tCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGAc                                        >  2:51297/1‑140 (MQ=255)                                                                                         tCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGAc                                        >  2:30404/1‑140 (MQ=255)                                                                                         tCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGAc                                        >  2:1023536/1‑140 (MQ=255)                                                                                         tCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGAc                                        >  2:714160/1‑140 (MQ=255)                                                                                         tCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGAc                                        >  1:371821/1‑140 (MQ=255)                                                                                         tCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTAGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCa                                                 <  2:1026822/131‑1 (MQ=255)                                                                                         tCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTAGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCa                                                 >  1:1026822/1‑131 (MQ=255)                                                                                         tCCTGCTGAAGTGGATGACCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGAc                                        >  2:739258/1‑140 (MQ=255)                                                                                           ctgctgAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACAt                                      <  1:116527/140‑1 (MQ=255)                                                                                                   gTGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTgg                              >  2:104296/1‑140 (MQ=255)                                                                                                    tGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGc                             <  1:1213181/140‑1 (MQ=255)                                                                                                    tGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGc                             <  1:137081/140‑1 (MQ=255)                                                                                                    tGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGc                             <  1:104296/140‑1 (MQ=255)                                                                                                    tGGATGATCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGc                             <  2:371821/140‑1 (MQ=255)                                                                                                      gatgatCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCgg                           <  1:714160/140‑1 (MQ=255)                                                                                                      gatgatCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCgg                           >  1:536348/1‑140 (MQ=255)                                                                                                       atgatCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGa                          <  1:336788/140‑1 (MQ=255)                                                                                                        tgatCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGat                         >  2:617204/1‑140 (MQ=255)                                                                                                        tgatCGCGGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGat                         <  2:916842/140‑1 (MQ=255)                                                                                                             gcgGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTaa                    <  1:1023536/140‑1 (MQ=255)                                                                                                             gcgGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTaa                    <  1:1128222/140‑1 (MQ=255)                                                                                                             gcgGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTaa                    <  1:1156051/140‑1 (MQ=255)                                                                                                             gcgGAAGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTaa                    <  1:177588/140‑1 (MQ=255)                                                                                                                 aaGGTTACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTAAagag                <  2:774260/140‑1 (MQ=255)                                                                                                                     ttACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGc                             <  1:438893/123‑1 (MQ=255)                                                                                                                     ttACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGc                             >  2:438893/1‑123 (MQ=255)                                                                                                                      tACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTAAAGAGCCAAt           >  1:1207151/1‑140 (MQ=255)                                                                                                                      tACGGCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGG‑AAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTAAAGAGCCAATc          >  1:1023772/1‑140 (MQ=255)                                                                                                                         ggCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTAAAGAGCCAATCCt        <  2:54260/140‑1 (MQ=255)                                                                                                                         ggCGATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTAAAGAGCCAATCCt        <  2:902540/140‑1 (MQ=255)                                                                                                                            gATCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTAAAGAGCCAATCCtgtg     <  2:21524/140‑1 (MQ=255)                                                                                                                             aTCGTCGTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTAAAGAGCCAATCCtgtgt    >  2:408988/1‑140 (MQ=255)                                                                                                                              tcgtcgTGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTAAAGAGCCAATCCtgtgtg   >  2:265582/1‑140 (MQ=255)                                                                                                                               cgtcgtGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTAAAGAGCCAAt           <  2:646117/131‑1 (MQ=255)                                                                                                                               cgtcgtGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTAAAGAGCCAAt           >  1:646117/1‑131 (MQ=255)                                                                                                                               cgtcgtGCCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTAAAGAGCCAATCCTGTGTGc  >  2:637957/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      ACCGATGCGTCCGCCGAGCGTTCTGCCGCTGGTTGTACCATCAAGCTGAACAAAGAACCGATCATCGAATACCTGAACTCTAACATCGTCCTGCTGAAGTGGATGATCGCGGAAGGTTACGGCGATCGTCGTACCCTGGAACGTCGTATTCAGGGCATGGAAAAATGGCTGGCGAATCCTGAGCTGCTGGAAGCCGATGCAGATGCGGAATACGCGGCAGTGATCGACATCGATCTGGCGGATATTAAAGAGCCAATCCTGTGTGC  >  NC_000913/133400‑133665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑