Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,410,385	C→T	A292V (GCC→GTC)	dbpA →	ATP‑dependent RNA helicase DbpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,410,385	0	C	T	100.0%	211.0 / NA	60	A292V (GCC→GTC)	dbpA	ATP‑dependent RNA helicase DbpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/26);  total (34/26)

TTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGCCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCA  >  NC_000913/1410249‑1410515                                                                                                                                         |                                                                                                                                   ttttGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCg                                                                                                                                 <  2:67504/140‑1 (MQ=255)         tACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTgg                                                                                                                         >  2:138399/1‑140 (MQ=255)                  aGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTg                                                                                                                <  2:633288/140‑1 (MQ=255)                              tGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTgcgc                                                                                                    <  2:758961/140‑1 (MQ=255)                               gTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGcgcg                                                                                                   <  1:458275/140‑1 (MQ=255)                                  tGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTgg                                                                                                >  2:30877/1‑140 (MQ=255)                                  tGCGACGCGCTGAATGAAGTACGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTgg                                                                                                >  1:695412/1‑140 (MQ=255)                                   gCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGt                                                                                               <  2:588251/140‑1 (MQ=255)                                     gACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCt                                                                                             <  1:1088700/140‑1 (MQ=255)                                      aCGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTg                                                                                            <  1:155227/140‑1 (MQ=255)                                                   aGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGc                                                                               <  1:1244874/140‑1 (MQ=255)                                                        ggCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTaa                                                                                    <  1:1060444/130‑1 (MQ=255)                                                        ggCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTaa                                                                                    >  2:1060444/1‑130 (MQ=255)                                                        ggCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAg                                                                          >  1:1200611/1‑140 (MQ=255)                                                        ggCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAg                                                                          >  1:687227/1‑140 (MQ=255)                                                        ggCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAg                                                                          >  1:365462/1‑140 (MQ=255)                                                        ggCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAg                                                                          >  1:908171/1‑140 (MQ=255)                                                        ggCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAg                                                                          >  1:232266/1‑140 (MQ=255)                                                        ggCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAg                                                                          >  2:281801/1‑140 (MQ=255)                                                        ggCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAg                                                                          >  2:295024/1‑140 (MQ=255)                                                          cAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTaa                                                                                    <  1:93724/128‑1 (MQ=255)                                                          cAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTaa                                                                                    >  2:93724/1‑128 (MQ=255)                                                          cAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCt                                                                        >  2:214346/1‑140 (MQ=255)                                                             aGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCtggt                                                                     >  2:664955/1‑140 (MQ=255)                                                               tGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGTTCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCtggtgg                                                                   >  1:514246/1‑140 (MQ=255)                                                                  aTTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGa                                                                >  1:491262/1‑140 (MQ=255)                                                                   ttGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGaa                                                               >  1:40226/1‑140 (MQ=255)                                                                         ttACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGa                                                         >  2:754012/1‑140 (MQ=255)                                                                         ttACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGa                                                         >  1:256560/1‑140 (MQ=255)                                                                                gCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCg                                                  >  1:724224/1‑140 (MQ=255)                                                                                gCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCg                                                  >  2:237127/1‑140 (MQ=255)                                                                                 cGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCgg                                                 >  1:1141009/1‑139 (MQ=255)                                                                                 cGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGt                                                 >  1:1006221/1‑140 (MQ=255)                                                                                        gAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGAccc                                          <  1:30877/140‑1 (MQ=255)                                                                                           cAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGa                                       <  2:1200611/140‑1 (MQ=255)                                                                                           cAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGa                                       <  1:237127/140‑1 (MQ=255)                                                                                           cAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGa                                       <  2:256560/140‑1 (MQ=255)                                                                                           cAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGa                                       >  1:174464/1‑140 (MQ=255)                                                                                             aCGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCg                                                  <  2:1058783/127‑1 (MQ=255)                                                                                             aCGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCg                                                  >  1:1058783/1‑127 (MQ=255)                                                                                             aCGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAg                                     <  2:147261/140‑1 (MQ=255)                                                                                             aCGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAg                                     <  2:695412/140‑1 (MQ=255)                                                                                              cgcgatcgcgatCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGTGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGt                                    >  1:50843/1‑140 (MQ=255)                                                                                              cgcgatcgcgatCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGt                                    >  2:1151020/1‑140 (MQ=255)                                                                                              cgcgatcgcgatCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGt                                    >  1:1122309/1‑140 (MQ=255)                                                                                              cgcgatcgcgatCAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGt                                    >  1:1221624/1‑140 (MQ=255)                                                                                                      cgatcAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTAc                            <  2:536277/140‑1 (MQ=255)                                                                                                        atcAGACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTAcat                          >  2:657880/1‑140 (MQ=255)                                                                                                            gACCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTAcatcgca                      <  1:295024/140‑1 (MQ=255)                                                                                                             aCCCTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTAcatcgcat                     <  1:657880/140‑1 (MQ=255)                                                                                                               ccTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTAcatcgc                       >  1:192588/1‑136 (MQ=255)                                                                                                               ccTGGTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTAcatcgc                       <  2:192588/136‑1 (MQ=255)                                                                                                                  ggTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTc                >  1:401108/1‑140 (MQ=255)                                                                                                                   gTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTAcatcgcat                     >  1:866861/1‑134 (MQ=255)                                                                                                                   gTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTAcatcgcat                     <  2:866861/134‑1 (MQ=255)                                                                                                                   gTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTAcatcgc                       >  1:1209459/1‑132 (MQ=255)                                                                                                                   gTACGTTTTGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTAcatcgc                       <  2:1209459/132‑1 (MQ=255)                                                                                                                         tttGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGc         <  2:514246/140‑1 (MQ=255)                                                                                                                           tGCTAACGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTc       <  2:401108/140‑1 (MQ=255)                                                                                                                                aCGGTAGCGTCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGTATCGGTCGTACAGCTCGTGCa  <  2:908171/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   TTTTGCAATACCAAAAAAGATTGCCAGGCTGTCTGCGACGCGCTGAATGAAGTAGGGCAAAGTGCATTGTCATTACACGGCGATTTGGAGCAACGCGATCGCGATCAGACCCTGGTACGTTTTGCTAACGGTAGCGCCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCA  >  NC_000913/1410249‑1410515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑