Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,431,622	C→T	V858V (GTC→GTT)	stfR →	Rac prophage; putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,431,622	0	C	T	100.0%	238.2 / NA	68	V858V (GTC→GTT)	stfR	Rac prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/40);  total (28/40)

TGGCTGCTTTTGCCAGAAGGGCAACGGATACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCAGAAGTTTATACCTCGAAAAATCTTCCACCAGAAAGC  >  NC_000913/1431486‑1431757                                                                                                                                         |                                                                                                                                        tgGCTGCTTTTGCCAGAAGGGCAACGGATACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGa                                                                                                                                      <  2:475992/140‑1 (MQ=255)      gctTTTGCCAGAAGGGCAACGGATACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTg                                                                                                                                 <  1:145306/140‑1 (MQ=255)           tGCCAGAAGGGCAACGGATACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGa                                                                                                                            >  2:706509/1‑140 (MQ=255)           tGCCAGAAGGGCAACGGATACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGa                                                                                                                            <  2:1020585/140‑1 (MQ=255)           tGCCAGAAGGGCAACGGATACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGa                                                                                                                            <  1:706509/140‑1 (MQ=255)            gCCAGAAGGGCAACGGATACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCg                                                                                                                              <  2:839185/137‑1 (MQ=255)            gCCAGAAGGGCAACGGATACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCg                                                                                                                              >  1:839185/1‑137 (MQ=255)                 aaGGGCAACGGATACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGc                                                                                                                      >  2:722678/1‑140 (MQ=255)                    ggCAACGGATACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGa                                                                                                                   >  2:107113/1‑140 (MQ=255)                    ggCAACGGATACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGa                                                                                                                   >  2:712161/1‑140 (MQ=255)                         cGGATACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAg                                                                                                              >  2:483029/1‑140 (MQ=255)                          ggATACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAgg                                                                                                             <  1:883392/140‑1 (MQ=255)                            aTACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGgcg                                                                                                           <  1:712279/140‑1 (MQ=255)                            aTACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGgcg                                                                                                           <  1:107113/140‑1 (MQ=255)                              aCATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCa                                                                                                         >  2:328404/1‑140 (MQ=255)                                   tGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACa                                                                                                    >  1:445160/1‑140 (MQ=255)                                    gCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTa                                                                                                      >  2:317720/1‑137 (MQ=255)                                    gCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTa                                                                                                      <  1:317720/137‑1 (MQ=255)                                    gCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAg                                                                                                   >  2:559590/1‑140 (MQ=255)                                     ccGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGa                                                                                                  >  1:565266/1‑140 (MQ=255)                                      cGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGgcg                                                                                                           >  1:446962/1‑130 (MQ=255)                                      cGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGgcg                                                                                                           <  1:888352/130‑1 (MQ=255)                                      cGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGgcg                                                                                                           <  2:446962/130‑1 (MQ=255)                                      cGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGgcg                                                                                                           >  2:888352/1‑130 (MQ=255)                                      cGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGaa                                                                                                 >  1:270987/1‑140 (MQ=255)                                      cGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGaa                                                                                                 <  2:1096185/140‑1 (MQ=255)                                         tGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAAtg                                                                                              >  2:169495/1‑140 (MQ=255)                                         tGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAAtg                                                                                              <  1:299171/140‑1 (MQ=255)                                            ggATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAAtggtg                                                                                           <  1:169495/140‑1 (MQ=255)                                              aTGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATggtggt                                                                                         >  2:148971/1‑140 (MQ=255)                                              aTGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATggtggt                                                                                         <  1:559590/140‑1 (MQ=255)                                              aTGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATggtggt                                                                                         <  1:760571/140‑1 (MQ=255)                                              aTGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATggtggt                                                                                         <  1:73673/140‑1 (MQ=255)                                              aTGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATggtggt                                                                                         <  2:812247/140‑1 (MQ=255)                                              aTGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATggtggt                                                                                         <  1:328404/140‑1 (MQ=255)                                              aTGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATggtggt                                                                                         <  1:27172/140‑1 (MQ=255)                                              aTGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATggtggt                                                                                         <  1:1042781/140‑1 (MQ=255)                                                 gtggCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTg                                                                                      <  1:1255838/140‑1 (MQ=255)                                                        tCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTAcc                                                                               >  1:823808/1‑140 (MQ=255)                                                            tGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGttc                                                                           >  2:525366/1‑140 (MQ=255)                                                              gAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGttctt                                                                         <  1:880238/140‑1 (MQ=255)                                                                   ccGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAaga                                                                    >  1:994975/1‑140 (MQ=255)                                                                   ccGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAaga                                                                    <  1:57771/140‑1 (MQ=255)                                                                    cGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAagag                                                                   <  1:722678/140‑1 (MQ=255)                                                                    cGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAagag                                                                   >  1:602131/1‑140 (MQ=255)                                                                       aTCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACg                                                                <  1:446598/140‑1 (MQ=255)                                                                           ggCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTa                                                            <  2:445160/140‑1 (MQ=255)                                                                                ttACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGtt                                                       <  1:483029/140‑1 (MQ=255)                                                                                ttACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGtt                                                       <  2:602131/140‑1 (MQ=255)                                                                                ttACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGtt                                                       <  1:525366/140‑1 (MQ=255)                                                                                          aCCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGa                                             <  2:1206171/140‑1 (MQ=255)                                                                                          aCCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGa                                             <  1:337347/140‑1 (MQ=255)                                                                                          aCCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGa                                             <  1:595206/140‑1 (MQ=255)                                                                                           cccGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGAc                                            <  2:435526/140‑1 (MQ=255)                                                                                                 ctGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCa                                      <  1:712161/140‑1 (MQ=255)                                                                                                 ctGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCa                                      <  2:1062136/140‑1 (MQ=255)                                                                                                 ctGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCa                                      <  1:148971/140‑1 (MQ=255)                                                                                                              tatTCTGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCAGAAGTTTATACCt                         >  1:489668/1‑140 (MQ=255)                                                                                                                   tGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCa                                      >  1:798438/1‑122 (MQ=255)                                                                                                                   tGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCa                                      <  2:798438/122‑1 (MQ=255)                                                                                                                   tGGTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCAGAAGTTTATACCTCGaaa                    <  2:565266/140‑1 (MQ=255)                                                                                                                     gTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCAGAAGTTTATACCTCGaaaaa                  >  1:475679/1‑140 (MQ=255)                                                                                                                     gTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCAGAAGTTTATACCTCGaaaaa                  >  2:872703/1‑140 (MQ=255)                                                                                                                     gTTAACTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAAAGACGGTTATGGTTTTGAGGAAGACTGGGCAGAAGTTTATACCTCGaaaaa                  >  2:1221727/1‑140 (MQ=255)                                                                                                                        aaCTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCAGAAGTTTATACCTCGAAAAATCt               >  1:1024045/1‑140 (MQ=255)                                                                                                                        aaCTTCTATACCGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCAGAAGTTTATACCTCGAAAAATCt               >  2:92090/1‑140 (MQ=255)                                                                                                                                  ccGGAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCAGAAGTTTATACCTCGAAAAATCTTCCACCAGaa     <  2:994975/140‑1 (MQ=255)                                                                                                                                     gAGTTGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCAGAAGTTTATACCTCGAAAAATCTTCCACCAGAAAGc  >  2:88924/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TGGCTGCTTTTGCCAGAAGGGCAACGGATACATATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGGTTTTGAGGAAGACTGGGCAGAAGTTTATACCTCGAAAAATCTTCCACCAGAAAGC  >  NC_000913/1431486‑1431757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑