Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,460,265	G→A	A404A (GCG→GCA)	paaH →	3‑hydroxyadipyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,460,265	0	G	A	100.0%	256.9 / NA	73	A404A (GCG→GCA)	paaH	3‑hydroxyadipyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/38);  total (35/38)

GTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAG  >  NC_000913/1460128‑1460400                                                                                                                                          |                                                                                                                                        gTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACt                                                                                                                                       >  1:803607/1‑140 (MQ=255)    aaCCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCa                                                                                                                                    >  1:198178/1‑140 (MQ=255)           aCTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGc                                                                                                                             <  1:542097/140‑1 (MQ=255)           aCTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGc                                                                                                                             >  1:831411/1‑140 (MQ=255)               aGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTgg                                                                                                                         >  2:1135215/1‑140 (MQ=255)                  gACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCct                                                                                                                      <  2:197694/140‑1 (MQ=255)                  gACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCct                                                                                                                      <  1:481835/140‑1 (MQ=255)                    cccGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCctct                                                                                                                    <  2:817088/140‑1 (MQ=255)                       gCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGaa                                                                                                                 >  1:191366/1‑140 (MQ=255)                       gCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGaa                                                                                                                 <  2:198178/140‑1 (MQ=255)                       gCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGaa                                                                                                                 <  2:803607/140‑1 (MQ=255)                       gCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGaa                                                                                                                 <  2:857550/140‑1 (MQ=255)                        cAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAAc                                                                                                                <  1:1135215/140‑1 (MQ=255)                          aGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAg                                                                                                              <  1:22811/140‑1 (MQ=255)                           ggCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAgg                                                                                                             >  1:1166148/1‑140 (MQ=255)                           ggCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAgg                                                                                                             >  1:316782/1‑140 (MQ=255)                             ccATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGat                                                                                                           <  1:993066/140‑1 (MQ=255)                              cATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTTCCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGaa                                                                                                                 >  1:348412/1‑133 (MQ=255)                              cATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTTCCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGaa                                                                                                                 <  2:348412/133‑1 (MQ=255)                              cATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGaa                                                                                                                 >  1:721187/1‑133 (MQ=255)                              cATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGaa                                                                                                                 <  2:721187/133‑1 (MQ=255)                              cATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGata                                                                                                          >  2:272906/1‑140 (MQ=255)                                      aCCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATAcc                                                                                                  >  1:166663/1‑140 (MQ=255)                                          gCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCa                                                                                              >  1:81200/1‑140 (MQ=255)                                          gCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCa                                                                                              >  2:439354/1‑140 (MQ=255)                                           cAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCTGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCAt                                                                                             >  2:809135/1‑140 (MQ=255)                                           cAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCAt                                                                                             <  1:242031/140‑1 (MQ=255)                                           cAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCAt                                                                                             <  1:188657/140‑1 (MQ=255)                                           cAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCAt                                                                                             >  2:1028156/1‑140 (MQ=255)                                                gcagGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTc                                                                                        <  2:54483/140‑1 (MQ=255)                                                gcagGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTc                                                                                        <  1:105450/140‑1 (MQ=255)                                                 cagGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCt                                                                                       >  2:815436/1‑140 (MQ=255)                                                     gCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTggg                                                                                   <  2:1067058/140‑1 (MQ=255)                                                     gCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTggg                                                                                   <  1:828167/140‑1 (MQ=255)                                                     gCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTggg                                                                                   <  1:439354/140‑1 (MQ=255)                                                       aaaaCAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGt                                                                                 <  2:831411/140‑1 (MQ=255)                                                              tgctgcAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTAt                                                                          >  1:110233/1‑140 (MQ=255)                                                              tgctgcAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTAt                                                                          <  1:272906/140‑1 (MQ=255)                                                                   cAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCata                                                                     >  1:147068/1‑140 (MQ=255)                                                                        tGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGcc                                                                >  1:945816/1‑140 (MQ=255)                                                                          cAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCa                                                              >  2:916698/1‑140 (MQ=255)                                                                          cAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCa                                                              <  2:191366/140‑1 (MQ=255)                                                                          cAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCa                                                              <  1:178727/140‑1 (MQ=255)                                                                          cAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCa                                                              <  2:416354/140‑1 (MQ=255)                                                                          cAGATTACCCAGGAATGCTGACTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCa                                                              <  2:1166148/140‑1 (MQ=255)                                                                                    aGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTggg                                                    <  2:945816/140‑1 (MQ=255)                                                                                         tGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAgcg                                               >  1:412128/1‑140 (MQ=255)                                                                                           cTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCa                                             >  2:58888/1‑140 (MQ=255)                                                                                                         ggTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGc                               <  2:81200/140‑1 (MQ=255)                                                                                                          gTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCg                              <  1:809135/140‑1 (MQ=255)                                                                                                          gTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCg                              <  2:611701/140‑1 (MQ=255)                                                                                                          gTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCg                              <  1:5347/140‑1 (MQ=255)                                                                                                          gTAGCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGAAATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCg                              <  1:369404/140‑1 (MQ=255)                                                                                                             gCAATGATCATCAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAat                           >  2:930040/1‑140 (MQ=255)                                                                                                                   atcatcAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAg                     >  1:820269/1‑140 (MQ=255)                                                                                                                   atcatcAATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAg                     >  2:173364/1‑140 (MQ=255)                                                                                                                    tcatcaATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAgg                    <  2:505437/140‑1 (MQ=255)                                                                                                                     catcaATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCg                              <  2:1104260/129‑1 (MQ=255)                                                                                                                     catcaATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCg                              >  1:1104260/1‑129 (MQ=255)                                                                                                                     catcaATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGc                   >  2:673490/1‑140 (MQ=255)                                                                                                                     catcaATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGc                   >  2:502691/1‑140 (MQ=255)                                                                                                                     catcaATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGc                   >  2:28253/1‑140 (MQ=255)                                                                                                                     catcaATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGACTGGCAGCGAATATTAAGGc                   >  2:342614/1‑140 (MQ=255)                                                                                                                      atcaATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGc                               <  1:832189/127‑1 (MQ=255)                                                                                                                      atcaATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGc                               <  1:903812/127‑1 (MQ=255)                                                                                                                      atcaATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGc                               >  2:832189/1‑127 (MQ=255)                                                                                                                      atcaATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGc                               >  2:903812/1‑127 (MQ=255)                                                                                                                      atcaATGAAGCCCTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCt                  <  2:316782/140‑1 (MQ=255)                                                                                                                        caATGAAGCCCTTGATGCACTTCAAAAAGGCATGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTcc                >  1:896904/1‑140 (MQ=255)                                                                                                                                  cTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCt      >  2:666703/1‑140 (MQ=255)                                                                                                                                  cTTGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCt      >  2:561908/1‑140 (MQ=255)                                                                                                                                    tGATGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTAc    <  1:666703/140‑1 (MQ=255)                                                                                                                                      aTGCACTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAg  <  2:820269/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        GTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGGCAAAACAGTGCTGCAAATTGCAGATTACCCAGGAATGCTGATTTGGCGAACGGTAGCAATGATCATCAATGAAGCCCTTGATGCGCTTCAAAAAGGCGTGGCCTCTGAACAGGATATCGATACCGCCATGCGTCTTGGGGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAG  >  NC_000913/1460128‑1460400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑