Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	144,925	G→A	V117I (GTC→ATC)	yadI →	putative PTS enzyme IIA component YadI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	144,925	0	G	A	100.0%	182.9 / NA	52	V117I (GTC→ATC)	yadI	putative PTS enzyme IIA component YadI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/24);  total (28/24)

TATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGTTT  >  NC_000913/144793‑145061                                                                                                                                     |                                                                                                                                         tATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACt                                                                                                                                   >  1:1080166/1‑140 (MQ=255)    cgcgTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTggg                                                                                                                                <  2:1232680/140‑1 (MQ=255)      cgTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACTAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTg                                                                                                                              <  1:998029/140‑1 (MQ=255)      cgTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCTAACTGGGTg                                                                                                                              >  1:456810/1‑140 (MQ=255)      cgTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTg                                                                                                                              >  1:694422/1‑140 (MQ=255)        tGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTgcg                                                                                                                            >  2:651345/1‑140 (MQ=255)        tGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTgcg                                                                                                                            >  2:217601/1‑140 (MQ=255)        tGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTgcg                                                                                                                            >  1:425609/1‑140 (MQ=255)         ggCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTgcgc                                                                                                                           >  2:910247/1‑140 (MQ=255)         ggCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTgcgc                                                                                                                           >  2:809265/1‑140 (MQ=255)           cTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCg                                                                                                                         >  2:1042514/1‑140 (MQ=255)           cTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCg                                                                                                                         >  2:375884/1‑140 (MQ=255)                   ttaAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGag                                                                                                                 <  1:432689/140‑1 (MQ=255)                     aaGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGagta                                                                                                               >  1:628310/1‑140 (MQ=255)                           caAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCttt                                                                                                         >  2:632842/1‑140 (MQ=255)                                  tCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACca                                                                                                  >  1:822629/1‑140 (MQ=255)                                          cGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACaaa                                                                                          >  2:1000512/1‑140 (MQ=255)                                                 aTTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATcc                                                                                   <  1:949424/140‑1 (MQ=255)                                                         tgtCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCg                                                                           <  1:217601/140‑1 (MQ=255)                                                         tgtCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCg                                                                           >  1:423363/1‑140 (MQ=255)                                                           tCACGTTACTGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTc                                                                         <  1:651345/140‑1 (MQ=255)                                                               gTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCa                                                                     <  1:192392/140‑1 (MQ=255)                                                                  aCCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAAc                                                                  >  2:610028/1‑140 (MQ=255)                                                                   ccGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACa                                                                 <  1:1149500/140‑1 (MQ=255)                                                                     gTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCTAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATa                                                               <  2:456810/140‑1 (MQ=255)                                                                             aaCAGATGATGGCTTGCCGTGAGACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTAt                                                       <  1:431695/140‑1 (MQ=255)                                                                             aaCAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTAt                                                       <  2:423363/140‑1 (MQ=255)                                                                             aaCAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTAt                                                       <  2:1080166/140‑1 (MQ=255)                                                                             aaCAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTAt                                                       <  1:1000512/140‑1 (MQ=255)                                                                             aaCAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTAt                                                       <  2:628310/140‑1 (MQ=255)                                                                             aaCAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTAt                                                       <  1:308300/140‑1 (MQ=255)                                                                             aaCAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTAt                                                       <  1:910247/140‑1 (MQ=255)                                                                             aaCAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTAt                                                       <  1:809265/140‑1 (MQ=255)                                                                             aaCAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTAt                                                       <  1:69158/140‑1 (MQ=255)                                                                             aaCAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTAt                                                       <  1:610028/140‑1 (MQ=255)                                                                                     atgGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTAt                                                       <  1:453299/132‑1 (MQ=255)                                                                                     atgGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTAt                                                       >  2:453299/1‑132 (MQ=255)                                                                                        gCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAAcc                                            <  2:564411/140‑1 (MQ=255)                                                                                          ttGCCGTGAAGCCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCcg                                          <  1:166920/140‑1 (MQ=255)                                                                                            gCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCcgcg                                        >  2:1103360/1‑140 (MQ=255)                                                                                            gCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCcgcg                                        >  2:445190/1‑140 (MQ=255)                                                                                            gCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCcgcg                                        >  1:245656/1‑140 (MQ=255)                                                                                              cGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGAt                                      >  1:698916/1‑140 (MQ=255)                                                                                                  aaaCCATGACCAGTTCAGAGTTTCGCGAGCGTATAATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGGCCTCAAACATAATGTGTATGAGTATTAACCCGCGCTTCTg                                  >  2:1133728/1‑140 (MQ=255)                                                                                                              gTTCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTg                      >  1:88123/1‑140 (MQ=255)                                                                                                               ttCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGc                     <  1:375884/140‑1 (MQ=255)                                                                                                               ttCAGAGTTTCGCGAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGc                     >  1:315708/1‑140 (MQ=255)                                                                                                                         cgcgAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATaaaa           >  1:589675/1‑140 (MQ=255)                                                                                                                         cgcgAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATaaaa           >  1:230178/1‑140 (MQ=255)                                                                                                                         cgcgAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATaaaa           >  1:26101/1‑140 (MQ=255)                                                                                                                         cgcgAGCGTATTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATaaaa           >  1:111236/1‑140 (MQ=255)                                                                                                                                  aTTATCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGttt  <  2:88123/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         TATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAACCCGCGATTCTGATGGCGCTTTTGCTACAATAAAAGCGTTGTTT  >  NC_000913/144793‑145061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑