| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,666,153 | A→G | N280S (AAC→AGC) | clcB → | putative chloride:H(+) antiporter ClcB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,666,153 | 0 | A | G | 98.2% | 184.5 / ‑4.3 | 54 | N280S (AAC→AGC) | clcB | putative chloride:H(+) antiporter ClcB |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (24/29); total (25/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01 | |||||||||||
ACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGA > NC_000913/1666019‑1666287 | aCCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCt > 2:352023/1‑140 (MQ=255) tgttgtTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGc > 2:1028941/1‑140 (MQ=255) tgttgtTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGc > 1:1218472/1‑140 (MQ=255) tgttgtTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGc > 2:38494/1‑140 (MQ=255) gttgttAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCa < 2:1200601/140‑1 (MQ=255) gttgttAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCa < 1:631070/140‑1 (MQ=255) gttgttAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCa < 2:349368/140‑1 (MQ=255) tAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTa < 2:338194/140‑1 (MQ=255) aaCGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTAc > 2:1098957/1‑140 (MQ=255) aaCGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTAc > 2:621227/1‑140 (MQ=255) aaCGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTAc > 2:397314/1‑140 (MQ=255) cGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACaa < 2:4620/140‑1 (MQ=255) aTGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCtt > 1:566064/1‑140 (MQ=255) aCGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTCCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTa < 2:196221/140‑1 (MQ=255) cGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTaa > 1:188844/1‑140 (MQ=255) gCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAAc < 1:529315/140‑1 (MQ=255) tCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGTTTGATCGTGGGTCTGCTTTCCCTGTTCCCCCCTGCAGTGTGGGGCAGCGGTTATAGCACCGTACAATCCTTTTTAACCGCCccac < 2:969900/140‑1 (MQ=255) tCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCCCCGTACAATCCTTTTTAACCGCCccac < 2:1130091/140‑1 (MQ=255) tCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCccac < 2:45209/140‑1 (MQ=255) tCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCccac < 2:829062/140‑1 (MQ=255) tCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCccac < 2:978811/140‑1 (MQ=255) cGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCccacc < 1:597799/140‑1 (MQ=255) cGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCccacc < 2:1153899/140‑1 (MQ=255) cGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGGGGGGAAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCccacc < 2:188844/140‑1 (MQ=255) gTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCccacca > 1:931872/1‑140 (MQ=255) gTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCccacca < 1:828781/140‑1 (MQ=255) gTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCccacca < 1:66750/140‑1 (MQ=255) tGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCcaccac < 1:999163/140‑1 (MQ=255) tttGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGtt > 1:210366/1‑140 (MQ=255) gtgAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAAt > 2:1016395/1‑140 (MQ=255) ctcAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCAt > 1:610397/1‑140 (MQ=255) aatcTTGCGCCGCCCTGGAAACTGGCACTGGGGGGGTTGATCGTGGGTCTGCTTTCCCTGTTCTCTCCTGCAGTGTGGTGCAGCGGGTATAGCACCGTACAATCATTTTTAACCGCCCCACCACTGTTAATGATCATTGc < 2:1144394/137‑1 (MQ=255) gcgcCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGAt > 1:336420/1‑140 (MQ=255) gcgcCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGAt > 1:262528/1‑140 (MQ=255) ccgccCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCtt < 1:1098957/140‑1 (MQ=255) ccgccCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCtt < 1:352023/140‑1 (MQ=255) gccCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTcc < 2:566064/140‑1 (MQ=255) cccTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCt > 1:1122925/1‑140 (MQ=255) cAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTaa < 1:9689/140‑1 (MQ=255) gCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTg > 1:612885/1‑140 (MQ=255) ggTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCgg < 2:612885/140‑1 (MQ=255) tttCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGgc > 2:1074756/1‑140 (MQ=255) ttCCCTGTTCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGgcg < 1:397314/140‑1 (MQ=255) tCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAGCTGTGTCCCGTGCTGGCGAGTAGCGGTTCCGGCGCCCCCGGt > 2:881878/1‑140 (MQ=255) tCACACCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGt > 2:469648/1‑140 (MQ=255) acacCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTgg < 2:29675/140‑1 (MQ=255) acacCTGCAGTGTGGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTgg < 2:931872/140‑1 (MQ=255) tgGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTgg > 1:4789/1‑128 (MQ=255) tgGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTgg < 2:4789/128‑1 (MQ=255) tgGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACAcc > 2:272823/1‑140 (MQ=255) tgGGGCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACAcc > 1:359028/1‑140 (MQ=255) ggggCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCg < 2:610397/140‑1 (MQ=255) gggCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGa < 1:1207876/140‑1 (MQ=255) gggCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGa > 1:1153974/1‑140 (MQ=255) gggCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGa > 1:496576/1‑140 (MQ=255) gggCAGCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGa < 1:1028941/140‑1 (MQ=255) | ACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGA > NC_000913/1666019‑1666287 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |