Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,669,062 G→A P177P (CCC→CCT)  ynfL ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,669,0620GA96.5% 188.2 / ‑2.0 58P177P (CCC→CCT) ynfLputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (28/28);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

ATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCG  >  NC_000913/1668928‑1669196
                                                                                                                                      |                                                                                                                                      
atgaCGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGgatg                                                                                                                                   >  2:643286/1‑140 (MQ=255)
atgaCGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGgatg                                                                                                                                   >  2:607237/1‑140 (MQ=255)
        cGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCg                                                                                                                           >  1:1216415/1‑140 (MQ=255)
        cGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCg                                                                                                                           >  1:266384/1‑140 (MQ=255)
              aTGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGatca                                                                                                                     <  1:643286/140‑1 (MQ=255)
              aTGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGatca                                                                                                                     <  2:344700/140‑1 (MQ=255)
                   aaCGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGcc                                                                                                                >  1:344253/1‑140 (MQ=255)
                   aaCGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGcc                                                                                                                >  1:318514/1‑140 (MQ=255)
                        cGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAACAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAg                                                                                                           <  1:739857/140‑1 (MQ=255)
                        cGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAg                                                                                                           <  2:709789/140‑1 (MQ=255)
                        cGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAg                                                                                                           <  2:245108/140‑1 (MQ=255)
                          cATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCg                                                                                                         >  1:49413/1‑140 (MQ=255)
                          cATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCg                                                                                                         >  1:352610/1‑140 (MQ=255)
                             cAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGtt                                                                                                      <  1:607237/140‑1 (MQ=255)
                                cccGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCAt                                                                                                   >  1:736706/1‑140 (MQ=255)
                                   gagaATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGa                                                                                                <  2:1216415/140‑1 (MQ=255)
                                   gagaATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGa                                                                                                <  2:266384/140‑1 (MQ=255)
                                   gagaATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAAAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGa                                                                                                >  2:306887/1‑140 (MQ=255)
                                           cGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACt                                                                                        <  2:352610/140‑1 (MQ=255)
                                               aTACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGa                                                                                          <  1:82796/134‑1 (MQ=255)
                                               aTACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGa                                                                                          <  1:386146/134‑1 (MQ=255)
                                               aTACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGa                                                                                          >  2:386146/1‑134 (MQ=255)
                                               aTACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGa                                                                                          >  2:82796/1‑134 (MQ=255)
                                                   aGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTc                                                                                >  2:43790/1‑140 (MQ=255)
                                                    gCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCa                                                                               >  2:340360/1‑140 (MQ=255)
                                                          tCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGc                                                                                      <  2:25110/127‑1 (MQ=255)
                                                          tCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGc                                                                                      >  1:25110/1‑127 (MQ=255)
                                                          tCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGAc                                                                         <  2:1150870/140‑1 (MQ=255)
                                                          tCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGAc                                                                         >  2:867741/1‑140 (MQ=255)
                                                          tCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGAc                                                                         >  1:749313/1‑140 (MQ=255)
                                                          tCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGAc                                                                         >  1:536469/1‑140 (MQ=255)
                                                               aCGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCgg                                                                    >  2:1055341/1‑140 (MQ=255)
                                                               aCGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCgg                                                                    >  2:1139451/1‑140 (MQ=255)
                                                               aCGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCgg                                                                    >  1:669814/1‑140 (MQ=255)
                                                               aCGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCgg                                                                    >  1:426174/1‑140 (MQ=255)
                                                                 gTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTa                                                                  <  2:669814/140‑1 (MQ=255)
                                                                    cGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGa                                                                          <  1:293227/129‑1 (MQ=255)
                                                                    cGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGa                                                                          >  2:293227/1‑129 (MQ=255)
                                                                    cGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACg                                                               >  2:502490/1‑140 (MQ=255)
                                                                    cGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACg                                                               >  2:1081413/1‑140 (MQ=255)
                                                                      gATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCt                                                             <  1:43790/140‑1 (MQ=255)
                                                                       aTCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCtg                                                            <  1:867741/140‑1 (MQ=255)
                                                                        tCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGGTCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGAc                                                                         <  2:102380/126‑1 (MQ=255)
                                                                        tCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGGTCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGAc                                                                         >  1:102380/1‑126 (MQ=255)
                                                                                gACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAAAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACgca                                                   <  1:306887/140‑1 (MQ=255)
                                                                                 aCAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAGGCGGCTCCGGTAACGCTGTGTTACgcgg                                                  <  2:287294/140‑3 (MQ=255)
                                                                                 aCAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACgcag                                                  <  1:33394/140‑1 (MQ=255)
                                                                                 aCAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACgcag                                                  <  1:1081413/140‑1 (MQ=255)
                                                                                   aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACgcagca                                                <  1:627546/140‑1 (MQ=255)
                                                                                   aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACgcagca                                                <  1:272235/140‑1 (MQ=255)
                                                                                   aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACgcagca                                                <  2:536469/140‑1 (MQ=255)
                                                                                   aaaGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACgcagca                                                <  1:813987/140‑1 (MQ=255)
                                                                                           cTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACGCAGCAATCCCata                                        >  2:1120175/1‑140 (MQ=255)
                                                                                                 ccAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACGCAGCAATCCCATATCCAGc                                  >  1:1105437/1‑140 (MQ=255)
                                                                                                                ttACATTCGGGTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACGCAGCAATCCCATATCCAGCGTTCCTTCAATgagc                   <  1:1139451/140‑1 (MQ=255)
                                                                                                                          gTTATTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGAttt         >  2:826953/1‑140 (MQ=255)
                                                                                                                             aTTTGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCt      >  1:427585/1‑140 (MQ=255)
                                                                                                                               ttGCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTcg    <  1:1055341/140‑1 (MQ=255)
                                                                                                                                 gCCAGAGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTcgcg  <  2:427585/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
ATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACGCAGCAATCCCATATCCAGCGTTCCTTCAATGAGCGGAGCGATTTGCTCGCG  >  NC_000913/1668928‑1669196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑