Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,700,710	T→C	V452V (GTT→GTC)	malX →	PTS enzyme IIBC component MalX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,700,710	0	T	C	100.0%	310.5 / NA	82	V452V (GTT→GTC)	malX	PTS enzyme IIBC component MalX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/53);  total (29/53)

GTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCG  >  NC_000913/1700575‑1700846                                                                                                                                        |                                                                                                                                         gTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTg                                                                                                                                      >  1:1115705/1‑140 (MQ=255)  tttGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGc                                                                                                                                     >  1:1265344/1‑140 (MQ=255)          ttACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCg                                                                                                                             <  1:837887/140‑1 (MQ=255)          ttACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCg                                                                                                                             <  1:655165/140‑1 (MQ=255)          ttACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCg                                                                                                                             <  1:524042/140‑1 (MQ=255)          ttACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCg                                                                                                                             <  1:514102/140‑1 (MQ=255)          ttACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCg                                                                                                                             <  1:337695/140‑1 (MQ=255)           tactacGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGa                                                                                                                            <  2:871947/140‑1 (MQ=255)                    aTCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAgg                                                                                                                   <  2:362481/140‑1 (MQ=255)                    aTCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAgg                                                                                                                   <  2:1265344/140‑1 (MQ=255)                    aTCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAgg                                                                                                                   >  1:735988/1‑140 (MQ=255)                     tCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAg                                                                                                                          <  2:37171/132‑1 (MQ=255)                     tCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAg                                                                                                                          >  1:37171/1‑132 (MQ=255)                          cGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGc                                                                                                             >  2:174666/1‑140 (MQ=255)                            tttCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCg                                                                                                           <  1:538664/140‑1 (MQ=255)                            tttCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCg                                                                                                           >  2:306907/1‑140 (MQ=255)                            tttCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCg                                                                                                           <  2:641191/140‑1 (MQ=255)                               cGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACa                                                                                                        >  1:628305/1‑140 (MQ=255)                               cGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACa                                                                                                        >  2:945182/1‑140 (MQ=255)                                      aCCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGt                                                                                                 <  2:735988/140‑1 (MQ=255)                                          gCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGc                                                                                             >  2:1027064/1‑140 (MQ=255)                                           cTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGcc                                                                                            >  1:689915/1‑140 (MQ=255)                                           cTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGcc                                                                                            >  1:158575/1‑140 (MQ=255)                                            ttCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCt                                                                                           <  2:574746/140‑1 (MQ=255)                                            ttCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCt                                                                                           >  1:52935/1‑140 (MQ=255)                                             tCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTc                                                                                          <  1:253682/140‑1 (MQ=255)                                                aTCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGAt                                                                                       >  1:362493/1‑140 (MQ=255)                                                aTCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGAt                                                                                       <  1:138501/140‑1 (MQ=255)                                                 tCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATa                                                                                      <  1:332429/140‑1 (MQ=255)                                                 tCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATa                                                                                      <  2:362493/140‑1 (MQ=255)                                                  cTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATaa                                                                                     <  2:378443/140‑1 (MQ=255)                                                  cTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATaa                                                                                     <  1:73450/140‑1 (MQ=255)                                                  cTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATaa                                                                                     <  2:196516/140‑1 (MQ=255)                                                   tGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCATGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAAc                                                                                    <  2:1148369/140‑1 (MQ=255)                                                     aaaaCCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTg                                                                                  >  2:688401/1‑140 (MQ=255)                                                     aaaaCCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTg                                                                                  >  1:226080/1‑140 (MQ=255)                                                     aaaaCCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTg                                                                                  >  2:818684/1‑140 (MQ=255)                                                           ccGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTAc                                                                            <  1:688401/140‑1 (MQ=255)                                                           ccGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTAc                                                                            >  1:693991/1‑140 (MQ=255)                                                               ggCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATa                                                                                      >  2:385314/1‑126 (MQ=255)                                                               ggCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATa                                                                                      <  1:385314/126‑1 (MQ=255)                                                               ggCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGt                                                                        >  2:244440/1‑140 (MQ=255)                                                               ggCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGt                                                                        <  2:952536/140‑1 (MQ=255)                                                               ggCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGt                                                                        >  1:335293/1‑140 (MQ=255)                                                                 cgcgATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCt                                                                      >  1:383633/1‑140 (MQ=255)                                                                   cgATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATa                                                                                      >  1:1012165/1‑122 (MQ=255)                                                                   cgATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATa                                                                                      <  2:1012165/122‑1 (MQ=255)                                                                      tAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGtt                                                                 <  1:766178/140‑1 (MQ=255)                                                                         cGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGt                                                              <  2:383633/140‑1 (MQ=255)                                                                         cGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGt                                                              <  2:158575/140‑1 (MQ=255)                                                                          gAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTc                                                             <  2:374896/140‑1 (MQ=255)                                                                          gAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTc                                                             <  2:187216/140‑1 (MQ=255)                                                                             gTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCtgt                                                          <  1:688002/140‑1 (MQ=255)                                                                              ttGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCtgt                                                          >  1:450452/1‑139 (MQ=255)                                                                              ttGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCtgt                                                          <  2:450452/139‑1 (MQ=255)                                                                                           cGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGc                                            <  2:1042872/140‑1 (MQ=255)                                                                                           cGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGc                                            <  2:221108/140‑1 (MQ=255)                                                                                                        gCCGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCa                               >  1:538677/1‑140 (MQ=255)                                                                                                         ccGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAg                              <  2:826019/140‑1 (MQ=255)                                                                                                         ccGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAg                              <  1:1274963/140‑1 (MQ=255)                                                                                                          cGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAgg                             <  1:1027064/140‑1 (MQ=255)                                                                                                          cGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAgg                             <  1:306907/140‑1 (MQ=255)                                                                                                          cGGTGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAgg                             <  2:1115705/140‑1 (MQ=255)                                                                                                             tGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCAc                          <  1:149809/140‑1 (MQ=255)                                                                                                             tGCGCCGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCCTGTTAATGTGCAGGCAc                          >  1:1240101/1‑140 (MQ=255)                                                                                                                  cGGGTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATATGCAGGCACTGAAg                     <  2:97201/140‑1 (MQ=255)                                                                                                                    ggTAAATCAGGTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGa                   <  2:52935/140‑1 (MQ=255)                                                                                                                              gTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCa         >  2:221519/1‑140 (MQ=255)                                                                                                                              gTTACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCa         >  2:1277497/1‑140 (MQ=255)                                                                                                                                tACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAAt       <  2:489004/140‑1 (MQ=255)                                                                                                                                tACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAAt       >  1:733902/1‑140 (MQ=255)                                                                                                                                tACAACGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAAt       <  1:174666/140‑1 (MQ=255)                                                                                                                                   aaCGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTgg    <  1:945182/140‑1 (MQ=255)                                                                                                                                   aaCGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTgg    <  1:818684/140‑1 (MQ=255)                                                                                                                                   aaCGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTgg    >  1:414725/1‑140 (MQ=255)                                                                                                                                   aaCGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTgg    <  1:144479/140‑1 (MQ=255)                                                                                                                                     cGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCg  <  2:226080/140‑1 (MQ=255)                                                                                                                                     cGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCg  <  2:510689/140‑1 (MQ=255)                                                                                                                                     cGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCg  <  2:628305/140‑1 (MQ=255)                                                                                                                                     cGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCg  <  2:689915/140‑1 (MQ=255)                                                                                                                                     cGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCg  <  1:244440/140‑1 (MQ=255)                                                                                                                                     cGTCCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTATGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCg  <  1:86678/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCG  >  NC_000913/1700575‑1700846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑