Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,752,659	T→C	Y391C (TAC→TGC)	ydhV ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,752,659	0	T	C	100.0%	258.3 / NA	68	Y391C (TAC→TGC)	ydhV	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/36);  total (32/36)

GACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAA  >  NC_000913/1752522‑1752795                                                                                                                                          |                                                                                                                                         gACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCaa                                                                                                                                        >  2:657518/1‑140 (MQ=255)     cAGCTCACCCACACGATGCGCCAGACGGTAGTAAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAAt                                                                                                                                    >  1:1157544/1‑140 (MQ=255)         tCACCCACACGATGCGCCAGACGGTAGTAAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGt                                                                                                                                >  2:479255/1‑140 (MQ=255)                   gATGCGCCAGACGGTAGTAAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGa                                                                                                                      <  1:574961/140‑1 (MQ=255)                     tGCGCCAGACGGTAGTAAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATg                                                                                                                    >  2:709591/1‑140 (MQ=255)                     tGCGCCAGACGGTAGTAAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATg                                                                                                                    >  2:73073/1‑140 (MQ=255)                             aCGGTAGTAAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGcc                                                                                                            <  1:112745/140‑1 (MQ=255)                             aCGGTAGTAAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGcc                                                                                                            <  1:709591/140‑1 (MQ=255)                               ggTAGTAAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCg                                                                                                          <  2:1157544/140‑1 (MQ=255)                               ggTAGTAAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCg                                                                                                          >  2:797170/1‑140 (MQ=255)                                gtagtaAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATGAGTAAAGTCGCGATGCAACTGCCCGt                                                                                                         <  2:462439/140‑1 (MQ=255)                                  agtaAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAg                                                                                                       <  1:946623/140‑1 (MQ=255)                                    taAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGtt                                                                                                     <  1:211256/140‑1 (MQ=255)                                    taAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGtt                                                                                                     >  2:981128/1‑140 (MQ=255)                                    taAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGt                                                                                                      <  1:842924/139‑1 (MQ=255)                                    taAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGt                                                                                                      >  2:842924/1‑139 (MQ=255)                                      aaaaTCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTac                                                                                                   >  1:178930/1‑140 (MQ=255)                                          tCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCa                                                                                               <  1:642809/140‑1 (MQ=255)                                           cTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCAt                                                                                              >  2:1107596/1‑140 (MQ=255)                                           cTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCAt                                                                                              >  2:847568/1‑140 (MQ=255)                                           cTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCAt                                                                                              <  2:193417/140‑1 (MQ=255)                                           cTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCAt                                                                                              >  2:262288/1‑140 (MQ=255)                                           cTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCAt                                                                                              >  1:41018/1‑140 (MQ=255)                                               aaTGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGc                                                                                          >  2:720105/1‑140 (MQ=255)                                                     gTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCAt                                                                                              <  1:290436/130‑1 (MQ=255)                                                     gTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCAt                                                                                              >  2:290436/1‑130 (MQ=255)                                                             cACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAAc                                                                            >  2:445793/1‑140 (MQ=255)                                                             cACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAAc                                                                            <  2:1141582/140‑1 (MQ=255)                                                               cccGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACaa                                                                          >  1:497412/1‑140 (MQ=255)                                                                ccGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACaaa                                                                         >  1:1242321/1‑140 (MQ=255)                                                                      ccAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGa                                                                   <  1:73073/140‑1 (MQ=255)                                                                      ccAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGa                                                                   <  2:497412/140‑1 (MQ=255)                                                                      ccAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGa                                                                   <  2:679509/140‑1 (MQ=255)                                                                         gTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGAccc                                                                <  2:72251/140‑1 (MQ=255)                                                                            gATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACccacc                                                             <  2:1000591/140‑1 (MQ=255)                                                                             aTCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACccacca                                                            <  1:657518/140‑1 (MQ=255)                                                                                ccAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGt                                                         <  2:1068322/140‑1 (MQ=255)                                                                                  aGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTa                                                       >  2:793720/1‑140 (MQ=255)                                                                                   gCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTAc                                                      <  1:1107596/140‑1 (MQ=255)                                                                                    cGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTAcc                                                     >  1:697698/1‑140 (MQ=255)                                                                                     gAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCa                                                    <  1:767600/140‑1 (MQ=255)                                                                                       aTTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAAt                                                  <  2:41018/140‑1 (MQ=255)                                                                                              cATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGa                                           <  1:1280872/140‑1 (MQ=255)                                                                                                 aCTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCa                                        <  2:1242321/140‑1 (MQ=255)                                                                                                 aCTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCa                                        <  1:262288/140‑1 (MQ=255)                                                                                                 aCTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCa                                        <  1:847568/140‑1 (MQ=255)                                                                                                 aCTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCa                                        <  2:459852/140‑1 (MQ=255)                                                                                                   tctTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATc                                      >  1:788492/1‑140 (MQ=255)                                                                                                     ttCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGt                                    >  2:526791/1‑140 (MQ=255)                                                                                                     ttCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGt                                    >  2:1117971/1‑140 (MQ=255)                                                                                                     ttCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGt                                    >  1:807443/1‑140 (MQ=255)                                                                                                        aGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCg                                     <  1:159135/136‑1 (MQ=255)                                                                                                        aGCTGGCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCg                                     >  2:159135/1‑136 (MQ=255)                                                                                                             gCAGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCt                            >  1:1196463/1‑140 (MQ=255)                                                                                                               aGAACACGCTTGAACACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTc                          >  1:651262/1‑140 (MQ=255)                                                                                                                           aaCACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCgg              >  2:327316/1‑140 (MQ=255)                                                                                                                           aaCACACCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCgg              >  2:909076/1‑140 (MQ=255)                                                                                                                            acacacCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGc             <  1:797170/140‑1 (MQ=255)                                                                                                                             cacacCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGcc            <  1:720105/140‑1 (MQ=255)                                                                                                                             cacacCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGcc            <  2:807443/140‑1 (MQ=255)                                                                                                                               cacCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCg          <  1:1117971/140‑1 (MQ=255)                                                                                                                                acCTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGt         >  1:906935/1‑140 (MQ=255)                                                                                                                                  cTTTGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTc       >  2:788869/1‑140 (MQ=255)                                                                                                                                    ttGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCgg              <  2:333868/131‑1 (MQ=255)                                                                                                                                    ttGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCgg              >  1:333868/1‑131 (MQ=255)                                                                                                                                    ttGCTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCgg     <  2:697698/140‑1 (MQ=255)                                                                                                                                     tgctgcAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCggg    >  2:727999/1‑140 (MQ=255)                                                                                                                                     tgctgcAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCggg    <  1:981128/140‑1 (MQ=255)                                                                                                                                       cTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGaa  <  1:445793/140‑1 (MQ=255)                                                                                                                                       cTGCAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGaa  <  1:526791/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAAAATCTTTAATGAAGTTAACGTCACCCGCTTCCAGTTGATCCCAGCGAATTTCTGCATACTCTTCAGCTGGCAGAACACGCTTGAACACACCTTTGCTGTAACAATAAGTAAAGTCGCGATGCAACTGCCCGTAGTTACACCATAGGCCGTAGTCATCGAACAAATTCAGACCCACCAGGTTACCAATCACACGACCATCGTCTTTATCTTCAAAATCTTTCGGCCCGTTCGGGAA  >  NC_000913/1752522‑1752795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑