Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,786,989	C→T	G42S (GGT→AGT)	ppsA ←	phosphoenolpyruvate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,786,989	0	C	T	100.0%	215.2 / NA	65	G42S (GGT→AGT)	ppsA	phosphoenolpyruvate synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/40);  total (25/40)

CTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTT  >  NC_000913/1786864‑1787125                                                                                                                              |                                                                                                                                         cTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGAt                                                                                                                            <  1:520598/140‑1 (MQ=255)   gAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTa                                                                                                                          <  1:200097/140‑1 (MQ=255)      tAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTa                                                                                                                       >  2:229893/1‑140 (MQ=255)       aaCATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTaa                                                                                                                      <  2:166812/140‑1 (MQ=255)           tCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGGAAACATTTGGGACGGGAAAACTCATTCCGGGAAGATTAGTAATCAt                                                                                                                  >  2:1262692/1‑140 (MQ=255)            cGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGGGGCGAAACCCTTCGGAACGGAAAAACCCATTCCCGAAAGATTAATAATCAtt                                                                                                                 >  2:313853/1‑140 (MQ=255)              tCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTaa                                                                                                                      >  1:35173/1‑133 (MQ=255)              tCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTaa                                                                                                                      <  2:35173/133‑1 (MQ=255)              tCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTc                                                                                                               >  2:610220/1‑140 (MQ=255)              tCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTc                                                                                                               >  2:538393/1‑140 (MQ=255)              tCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTAAGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTc                                                                                                               >  1:665874/1‑140 (MQ=255)                    tCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAg                                                                                                         >  1:266561/1‑140 (MQ=255)                     ccGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAgg                                                                                                        <  1:44525/140‑1 (MQ=255)                        ttttATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAg                                                                                                     >  2:188452/1‑140 (MQ=255)                          ttATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAAACATTCGGAACGGGAAAACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGc                                                                                                   >  1:958338/1‑140 (MQ=255)                            aTCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCAt                                                                                                 >  2:589447/1‑140 (MQ=255)                                agcagTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCAttttt                                                                                             <  2:355021/140‑1 (MQ=255)                                     ttCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGcccc                                                                                        <  1:1042159/140‑1 (MQ=255)                                       cATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCa                                                                                      >  1:267889/1‑140 (MQ=255)                                       cATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCa                                                                                      >  2:336188/1‑140 (MQ=255)                                         tAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAAc                                                                                    <  1:667607/140‑1 (MQ=255)                                          aaaTGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAAcc                                                                                   <  1:586355/140‑1 (MQ=255)                                          aaaTGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAAcc                                                                                   <  1:723988/140‑1 (MQ=255)                                          aaaTGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAAcc                                                                                   <  2:405734/140‑1 (MQ=255)                                          aaaTGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAAcc                                                                                   <  1:229893/140‑1 (MQ=255)                                          aaaTGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAAcc                                                                                   <  2:525812/140‑1 (MQ=255)                                          aaaTGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAAcc                                                                                   <  2:684374/140‑1 (MQ=255)                                          aaaTGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAAcc                                                                                   <  2:703510/140‑1 (MQ=255)                                               cgcTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTc                                                                              >  1:1196973/1‑140 (MQ=255)                                               cgcTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGGAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTc                                                                              >  2:258009/1‑140 (MQ=255)                                                 cTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGcccc                                                                                        <  2:98310/128‑1 (MQ=255)                                                 cTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGcccc                                                                                        >  1:98310/1‑128 (MQ=255)                                                 cTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGGGGCGAAACCATTCGGAACGGAAACACTCATTTCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTa                                                                            >  1:306836/1‑140 (MQ=255)                                                            cgcTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATg                                                                 <  1:1262692/140‑1 (MQ=255)                                                              cTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGcc                                                               >  2:566697/1‑140 (MQ=255)                                                              cTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGcc                                                               >  2:1167760/1‑140 (MQ=255)                                                              cTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGc                                                                <  1:422273/139‑1 (MQ=255)                                                              cTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGc                                                                >  2:422273/1‑139 (MQ=255)                                                              cTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGGAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGcc                                                               >  1:816608/1‑140 (MQ=255)                                                                 tGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAg                                                            <  1:566697/140‑1 (MQ=255)                                                                 tGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAg                                                            <  2:694709/140‑1 (MQ=255)                                                                 tGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAg                                                            <  1:538393/140‑1 (MQ=255)                                                                 tGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAg                                                            <  1:589447/140‑1 (MQ=255)                                                                 tGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAg                                                            <  1:381544/140‑1 (MQ=255)                                                                 tGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAAACATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAg                                                            <  2:1196973/140‑1 (MQ=255)                                                                   gTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGtt                                                          >  1:452796/1‑140 (MQ=255)                                                                       aGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGtt                                                      <  1:258009/140‑1 (MQ=255)                                                                        gAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTta                                                     <  1:71958/140‑1 (MQ=255)                                                                             tGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTtat                                                    >  1:495759/1‑136 (MQ=255)                                                                             tGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTtat                                                    <  2:495759/136‑1 (MQ=255)                                                                             tGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCa                                                >  1:1074159/1‑140 (MQ=255)                                                                              ggTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCaa                                               <  1:188452/140‑1 (MQ=255)                                                                               gTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCaaa                                              >  2:183201/1‑140 (MQ=255)                                                                                        gTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCg                                     <  1:336188/140‑1 (MQ=255)                                                                                         tCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCgg                                    <  1:610220/140‑1 (MQ=255)                                                                                            gcggTTGTGGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGa                                 >  2:170393/1‑140 (MQ=255)                                                                                                 tgtgGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGc                            <  1:985026/140‑1 (MQ=255)                                                                                                 tgtgGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGc                            <  2:665874/140‑1 (MQ=255)                                                                                                   tgGCGAAACCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCa                          <  2:450415/140‑1 (MQ=255)                                                                                                        aaaCCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCAttgtt                     <  2:664246/140‑1 (MQ=255)                                                                                                        aaaCCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCAttgtt                     <  2:452796/140‑1 (MQ=255)                                                                                                          aCCATTCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCC‑TTGTTGGa                  >  1:1194421/1‑140 (MQ=255)                                                                                                              ttCGGAACGGAAACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCACCCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACAt               <  1:313853/140‑1 (MQ=255)                                                                                                                cggaacggaaACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCg             <  2:451707/140‑1 (MQ=255)                                                                                                                  gaacggaaACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAGCCCTTTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGaa           <  2:274174/140‑1 (MQ=255)                                                                                                                  gaacggaaACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGaa           <  1:1167760/140‑1 (MQ=255)                                                                                                                     cggaaACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCa                          <  1:161807/122‑1 (MQ=255)                                                                                                                     cggaaACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCa                          >  2:161807/1‑122 (MQ=255)                                                                                                                      ggaaACACTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAAt       >  1:1049437/1‑140 (MQ=255)                                                                                                                           cacTCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCttt  <  2:1074159/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                         CTGAGTAACATCGTCAATATCCGTTTTATCCAGCAGTTCATAAATGCGCTGGTTTACGCCGCTTTGGTCCAGAAACTGGTTAAACGCGTCGGCGGTTGTGGCGAAACCATTCGGAACGGAAACACCCATTCCGGAAAGATTAGTAATCATTTCACCCAGGGAGGCATTTTTGCCCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTT  >  NC_000913/1786864‑1787125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑