Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	193,408	A→G	E179E (GAA→GAG)	frr →	ribosome‑recycling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	193,408	0	A	G	100.0%	155.7 / NA	43	E179E (GAA→GAG)	frr	ribosome‑recycling factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/20);  total (23/20)

AAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCACCATTCTG  >  NC_000913/193289‑193541                                                                                                                        |                                                                                                                                      aaaGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTg                                                                                                                   <  1:518633/140‑1 (MQ=255)        tGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGGGCTGGCAGACAAAGAGGCAGAACTGATGCGGTTCTGATTTCtt                                                                                                            >  2:1189266/1‑140 (MQ=255)         gTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTg                                                                                                           <  1:715446/140‑1 (MQ=255)          ttGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGa                                                                                                          >  1:504327/1‑140 (MQ=255)                    aaGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACaaaaa                                                                                                <  2:460055/140‑1 (MQ=255)                    aaGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACaaaaa                                                                                                >  2:306724/1‑140 (MQ=255)                      gagaTCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAAcg                                                                                              <  2:1053049/140‑1 (MQ=255)                         aTCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAAcgccg                                                                                           <  2:504327/140‑1 (MQ=255)                         aTCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAAcgccg                                                                                           <  1:235153/140‑1 (MQ=255)                         aTCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAAcgccg                                                                                           <  1:369893/140‑1 (MQ=255)                         aTCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAAcgccg                                                                                           <  1:518850/140‑1 (MQ=255)                           cAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCGGTTCTGATTTCTTGAACGACAAAAACGCCGCt                                                                                         <  1:1189266/140‑1 (MQ=255)                           cAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTTATTTCTTGAACGACAAAAACGCCGCt                                                                                         >  1:94194/1‑140 (MQ=255)                                            gCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGa                                                                        >  1:505545/1‑140 (MQ=255)                                                            tGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGttt                                                        >  1:433323/1‑140 (MQ=255)                                                            tGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGttt                                                        >  2:805069/1‑140 (MQ=255)                                                            tGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGttt                                                        >  2:439493/1‑140 (MQ=255)                                                            tGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGtt                                                         >  1:633314/1‑139 (MQ=255)                                                            tGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGtt                                                         <  2:633314/139‑1 (MQ=255)                                                                    aaCTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCttttt                                                >  2:325909/1‑140 (MQ=255)                                                                      ctgactgaTGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTAt                                              >  2:442899/1‑140 (MQ=255)                                                                      ctgactgaTGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTAt                                              >  2:1176143/1‑140 (MQ=255)                                                                      ctgactgaTGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGCTTTGCTTTTTAt                                              >  1:1239907/1‑140 (MQ=255)                                                                      ctgactgaTGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATGTCTTGAACGACAGAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTAt                                              >  2:1111594/1‑140 (MQ=255)                                                                        gactgaTGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTAttt                                            >  2:452095/1‑139 (MQ=255)                                                                        gactgaTGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTc                                            >  2:1179788/1‑140 (MQ=255)                                                                        gactgaTGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTc                                            >  1:1019098/1‑140 (MQ=255)                                                                        gactgaTGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTc                                            >  1:376561/1‑140 (MQ=255)                                                                             aTGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGtct                                       <  1:306724/140‑1 (MQ=255)                                                                              tgctgcAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGtctc                                      <  1:442899/140‑1 (MQ=255)                                                                                    aaTCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAActct                                <  1:325909/140‑1 (MQ=255)                                                                                      tCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTgg                              <  2:687098/140‑1 (MQ=255)                                                                                        aacaaaaTTGAAGCGGCGCTGGCAGACAAAGAGGCAGCACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTC‑‑‑AGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGAt                            <  2:1133287/134‑1 (MQ=255)                                                                                        aaGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGAt                            >  2:98042/1‑140 (MQ=255)                                                                                        aaGAAAATTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTC‑‑‑AGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGAt                            >  1:1133287/1‑137 (MQ=255)                                                                                             aaTTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTc                       >  2:912065/1‑140 (MQ=255)                                                                                              aTTGAAGCGGCGCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCa                      <  2:433323/140‑1 (MQ=255)                                                                                                     cggcgCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCa               <  2:447634/140‑1 (MQ=255)                                                                                                     cggcgCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCa               <  2:376561/140‑1 (MQ=255)                                                                                                     cggcgCTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCa               <  1:98042/140‑1 (MQ=255)                                                                                                        cgcTGGCAGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACt            >  2:790653/1‑140 (MQ=255)                                                                                                               aGACAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCACCAtt     <  2:941113/140‑1 (MQ=255)                                                                                                                  cAAAGAGGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCACCATTCTg  >  2:704638/1‑140 (MQ=255)                                                                                                                        |                                                                                                                                      AAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCACCATTCTG  >  NC_000913/193289‑193541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑