Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,867,413	G→A	R169H (CGC→CAC)	yeaG →	protein kinase YeaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,867,413	0	G	A	98.7%	257.1 / ‑6.7	75	R169H (CGC→CAC)	yeaG	protein kinase YeaG
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (41/33);  total (42/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACGGAACC  >  NC_000913/1867279‑1867548                                                                                                                                       |                                                                                                                                        aTCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGtt                                                                                                                                    <  1:211304/140‑1 (MQ=255)     ttAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTAtct                                                                                                                                <  2:519721/140‑1 (MQ=255)        aTGCAGCTCGTACCGATTTATGTATTGAGCTCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCgg                                                                                                                             <  2:353912/140‑1 (MQ=255)             gCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACca                                                                                                                        >  2:150516/1‑140 (MQ=255)              cTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACcat                                                                                                                       >  2:659583/1‑140 (MQ=255)                  tACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATg                                                                                                                   >  2:1066064/1‑140 (MQ=255)                        tttATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCg                                                                                                             >  1:898309/1‑140 (MQ=255)                          tATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTg                                                                                                           <  1:1116855/140‑1 (MQ=255)                             gTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGggc                                                                                                        <  1:128315/140‑1 (MQ=255)                                   aGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTgg                                                                                                          <  2:875816/132‑1 (MQ=255)                                   aGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTgg                                                                                                          >  1:875816/1‑132 (MQ=255)                                   aGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCaaa                                                                                                  >  1:127007/1‑140 (MQ=255)                                   aGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCaaa                                                                                                  >  1:672078/1‑140 (MQ=255)                                    gcgcGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCaaaa                                                                                                 <  2:898309/140‑1 (MQ=255)                                     cgcgAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAAc                                                                                                >  1:949560/1‑140 (MQ=255)                                     cgcgAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAAc                                                                                                >  2:1128058/1‑140 (MQ=255)                                     cgcgAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAAc                                                                                                >  1:515843/1‑140 (MQ=255)                                      gcgAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACg                                                                                               >  2:186042/1‑140 (MQ=255)                                       cgAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGc                                                                                              >  2:1135748/1‑140 (MQ=255)                                        gAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGcc                                                                                             >  2:92755/1‑140 (MQ=255)                                        gAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGcc                                                                                             <  1:914857/140‑1 (MQ=255)                                        gAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGcc                                                                                             <  2:1181935/140‑1 (MQ=255)                                                  cGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAAtt                                                                                   >  1:951316/1‑140 (MQ=255)                                                  cGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAAtt                                                                                   >  1:732475/1‑140 (MQ=255)                                                  cGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAAtt                                                                                   >  1:697110/1‑140 (MQ=255)                                                    tAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTtg                                                                                 >  1:480759/1‑140 (MQ=255)                                                    tAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTtg                                                                                 <  1:87002/140‑1 (MQ=255)                                                      gCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTtggt                                                                               >  2:1244650/1‑140 (MQ=255)                                                      gCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTtggt                                                                               <  1:907990/140‑1 (MQ=255)                                                       cccGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTtggtg                                                                              >  1:1121082/1‑140 (MQ=255)                                                        ccGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTtggtgg                                                                             <  1:1244650/140‑1 (MQ=255)                                                          ggTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCg                                                                           <  2:58963/140‑1 (MQ=255)                                                           gTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGa                                                                          >  1:1240079/1‑140 (MQ=255)                                                            tCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGat                                                                         >  2:689491/1‑140 (MQ=255)                                                            tCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGat                                                                         >  2:383294/1‑140 (MQ=255)                                                             cAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGata                                                                        <  2:1198087/140‑1 (MQ=255)                                                             cAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGata                                                                        <  2:127007/140‑1 (MQ=255)                                                                 gATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCA‑CATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACt                                                                   <  1:1233110/140‑1 (MQ=255)                                                                  atcatcCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACt                                                                   <  2:703633/140‑1 (MQ=255)                                                                     atcCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAg                                                                >  2:338872/1‑140 (MQ=255)                                                                      tcCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGt                                                               >  2:1174153/1‑140 (MQ=255)                                                                      tcCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGt                                                               >  2:509810/1‑140 (MQ=255)                                                                      tcCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGt                                                               >  1:386041/1‑140 (MQ=255)                                                                       ccGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGtt                                                              <  2:697110/140‑1 (MQ=255)                                                                       ccGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGtt                                                              <  2:951316/140‑1 (MQ=255)                                                                        cGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCCGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTc                                                             <  2:1126782/140‑1 (MQ=255)                                                                            cTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCggg                                                         <  1:338872/140‑1 (MQ=255)                                                                             tGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGt                                                        >  1:342238/1‑140 (MQ=255)                                                                               tCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACt                                                                   <  2:388036/127‑1 (MQ=255)                                                                               tCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACt                                                                   >  1:388036/1‑127 (MQ=255)                                                                                cTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGt                                                     <  1:1066064/140‑1 (MQ=255)                                                                                  tttCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGa                                                   <  2:342238/140‑1 (MQ=255)                                                                                  tttCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGa                                                   <  1:150516/140‑1 (MQ=255)                                                                                    tCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAg                                                 >  1:1014277/1‑140 (MQ=255)                                                                                    tCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAg                                                 >  2:500631/1‑140 (MQ=255)                                                                                       aTCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTc                                              <  2:949560/140‑1 (MQ=255)                                                                                         ccGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTg                                            >  2:168085/1‑140 (MQ=255)                                                                                         ccGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTg                                            >  2:427660/1‑140 (MQ=255)                                                                                         ccGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTg                                            >  1:445749/1‑140 (MQ=255)                                                                                                    tGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTc                                 >  1:350743/1‑140 (MQ=255)                                                                                                         aGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGcaa                            <  2:206366/140‑1 (MQ=255)                                                                                                         aGATTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGcaa                            >  2:619815/1‑140 (MQ=255)                                                                                                           aTTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGcaaca                          >  2:444793/1‑140 (MQ=255)                                                                                                           aTTCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGcaaca                          >  1:165712/1‑140 (MQ=255)                                                                                                             tCTGGAAAAAGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACaaa                        <  2:165712/140‑1 (MQ=255)                                                                                                                  aaaaaGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCt                   <  1:444793/140‑1 (MQ=255)                                                                                                                  aaaaaGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCt                   <  1:1174153/140‑1 (MQ=255)                                                                                                                     aaGAGTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATc                >  2:1023838/1‑140 (MQ=255)                                                                                                                       gagTATGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGc              <  1:689491/140‑1 (MQ=255)                                                                                                                            tGGCATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCaaaa         >  1:501642/1‑140 (MQ=255)                                                                                                                               cATTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACgg      >  1:462719/1‑140 (MQ=255)                                                                                                                                aTTCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACGGa     <  1:659583/140‑1 (MQ=255)                                                                                                                                  tCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTAt                 <  1:559220/126‑1 (MQ=255)                                                                                                                                  tCCTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTAt                 >  2:559220/1‑126 (MQ=255)                                                                                                                                   ccTCACCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACGGAAcc  >  2:16737/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        ATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACGGAACC  >  NC_000913/1867279‑1867548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑