Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,067,300	C→T	G8R (GGA→AGA)	insH‑6 ←	CP4‑44 prophage; IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,067,300	0	C	T	100.0%	83.9 / NA	25	G8R (GGA→AGA)	insH‑6	CP4‑44 prophage; IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/15);  total (10/15)

CTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCACAGCATTCAACAGAATGTGACTTGCCAGATTTATTAG  >  NC_000913/2067222‑2067431                                                                               |                                                                                                                                    ctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAaca                                                                        >  1:262783/1‑140 (MQ=18)           cTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTc                                                              <  2:346159/140‑1 (MQ=255)            tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCg                                                             >  2:957586/1‑140 (MQ=255)            tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCg                                                             <  2:687282/140‑1 (MQ=255)            tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCg                                                             >  1:69318/1‑140 (MQ=255)              aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGct                                                           >  2:465365/1‑140 (MQ=255)               cGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGctc                                                          <  1:465365/140‑1 (MQ=255)                ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGctct                                                         <  1:613818/140‑1 (MQ=255)                ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGctct                                                         <  2:69318/140‑1 (MQ=255)                   gcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCtctctc                                                      >  2:1112771/1‑140 (MQ=255)                    cTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCg                                                     >  2:967647/1‑140 (MQ=255)                      tACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGt                                                   <  1:527287/140‑1 (MQ=255)                                   aCTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGctctct                                                       <  2:624168/123‑1 (MQ=255)                                   aCTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGctctct                                                       >  1:624168/1‑123 (MQ=255)                                   aCTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTcac                                      >  2:487009/1‑140 (MQ=255)                                    cTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTcaca                                     <  2:459331/140‑1 (MQ=255)                                      gTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTca                                       <  2:662432/136‑1 (MQ=255)                                      gTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTca                                       >  1:662432/1‑136 (MQ=255)                                        cGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCACAGCAt                                 <  2:144995/140‑1 (MQ=255)                                            gAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCACAGCATTCaa                             <  2:334389/140‑1 (MQ=255)                                            gAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCACAGCATTCaa                             <  1:894205/140‑1 (MQ=255)                                            gAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCACAGCATTCaa                             <  2:631412/140‑1 (MQ=255)                                                 tAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGCACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCACAGCATTCAACAGaa                        <  2:791133/140‑1 (MQ=255)                                                                   tgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCACAGCATTCAACAGAATGTGACTTGCCAGATtta      <  1:670617/140‑1 (MQ=255)                                                                       cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCACAGCATTCAACAGAATGTGACTTGCCAGATTTATTAg  >  2:269279/1‑140 (MQ=255)                                                                               |                                                                                                                                    CTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCACAGCATTCAACAGAATGTGACTTGCCAGATTTATTAG  >  NC_000913/2067222‑2067431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑