Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,150,824	T→C	K54K (AAA→AAG)	yegI ←	protein kinase YegI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,150,824	0	T	C	100.0%	323.6 / NA	87	K54K (AAA→AAG)	yegI	protein kinase YegI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/55);  total (32/55)

GAATCGGTTCTTTACCAGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGTTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTC  >  NC_000913/2150688‑2150956                                                                                                                                         |                                                                                                                                     gAATCGGTTCTTTACCAGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTg                                                                                                                                   >  2:1151693/1‑140 (MQ=255) gAATCGGTTCTTTACCAGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTg                                                                                                                                   >  2:720875/1‑140 (MQ=255) gAATCGGTTCTTTACCAGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTg                                                                                                                                   >  2:1147968/1‑140 (MQ=255)   aTCGGTTCTTTACCAGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTc                                                                                                                                 <  1:150382/140‑1 (MQ=255)   aTCGGTTCTTTACCAGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTc                                                                                                                                 <  2:938008/140‑1 (MQ=255)   aTCGGTTCTTTACCAGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTc                                                                                                                                 <  2:942081/140‑1 (MQ=255)   aTCGGTTCTTTACCAGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCACAGCCATAAAGGCAAGCTTGTc                                                                                                                                 <  1:171638/140‑1 (MQ=255)      ggTTCTTTACCAGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTg                                                                                                                              >  1:945553/1‑140 (MQ=255)       gTTCTTTACCAGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGt                                                                                                                             >  1:780017/1‑140 (MQ=255)        ttCTTTACCAGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGtt                                                                                                                            <  1:353907/140‑1 (MQ=255)         tCTTTACCAGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGttt                                                                                                                           >  1:365746/1‑140 (MQ=255)                 aGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCgg                                                                                                                   <  1:419191/140‑1 (MQ=255)                 aGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCgg                                                                                                                   <  1:405797/140‑1 (MQ=255)                  gAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTc                                                                                                                                 <  2:1127199/125‑1 (MQ=255)                  gAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTc                                                                                                                                 >  1:1127199/1‑125 (MQ=255)                               tcatAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGa                                                                                                     <  2:602366/140‑1 (MQ=255)                                catAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGAt                                                                                                    <  2:945553/140‑1 (MQ=255)                                 atAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATa                                                                                                   >  2:1108106/1‑140 (MQ=255)                                 atAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATa                                                                                                   >  1:444423/1‑140 (MQ=255)                                          aTAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGc                                                                                          >  1:763443/1‑140 (MQ=255)                                          aTAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGc                                                                                          >  1:281496/1‑140 (MQ=255)                                          aTAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGc                                                                                          >  2:588411/1‑140 (MQ=255)                                          aTAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGc                                                                                          >  1:738120/1‑140 (MQ=255)                                          aTAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGc                                                                                          >  2:733381/1‑140 (MQ=255)                                          aTAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAACCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGgcggcg                                                                                                          >  1:346919/1‑124 (MQ=255)                                          aTAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAACCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGgcggcg                                                                                                          <  2:346919/124‑1 (MQ=255)                                           tAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCg                                                                                         <  2:106613/140‑1 (MQ=255)                                           tAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCg                                                                                         >  2:1221995/1‑140 (MQ=255)                                           tAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCg                                                                                         <  2:623946/140‑1 (MQ=255)                                            agcTTTTCCGCCTCGTCCACCGGGAAGCGTTGCCTGCGGCCAGGCGACATAATGCAACAACTGCGCGTCAGCTGTCGCAGCAATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGa                                                                                        <  2:490241/138‑1 (MQ=255)                                                 ttCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTa                                                                                   >  2:136104/1‑140 (MQ=255)                                                       cTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACa                                                                             <  1:907116/140‑1 (MQ=255)                                                       cTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACa                                                                             <  2:141164/140‑1 (MQ=255)                                                        tCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACaa                                                                            <  1:257228/140‑1 (MQ=255)                                                         cGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACaaa                                                                           >  2:1180610/1‑140 (MQ=255)                                                         cGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACaaa                                                                           >  2:1196743/1‑140 (MQ=255)                                                          gTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAAc                                                                          <  1:526082/140‑1 (MQ=255)                                                            ccACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAActc                                                                        <  2:444423/140‑1 (MQ=255)                                                              aCCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAActcct                                                                      <  2:1185102/140‑1 (MQ=255)                                                              aCCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAActcct                                                                      <  2:937606/140‑1 (MQ=255)                                                                cGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCg                                                                    <  1:723790/140‑1 (MQ=255)                                                                cGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCg                                                                    <  2:269831/140‑1 (MQ=255)                                                                cGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCg                                                                    <  1:362165/140‑1 (MQ=255)                                                                cGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCg                                                                    <  1:1151693/140‑1 (MQ=255)                                                                cGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCg                                                                    <  1:1108106/140‑1 (MQ=255)                                                                cGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCg                                                                    <  1:1013802/140‑1 (MQ=255)                                                                cGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCATGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCg                                                                    <  1:304239/140‑1 (MQ=255)                                                                  tGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACaaa                                                                           <  1:69466/131‑1 (MQ=255)                                                                  tGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACaaa                                                                           >  2:69466/1‑131 (MQ=255)                                                                  tGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGat                                                                  >  2:136304/1‑140 (MQ=255)                                                                     aGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATc                                                               >  2:956269/1‑140 (MQ=255)                                                                         ttGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATaa                                                           >  2:641706/1‑140 (MQ=255)                                                                             cTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACcg                                                       <  1:1254486/140‑1 (MQ=255)                                                                               gCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACcgcg                                                     <  1:136104/140‑1 (MQ=255)                                                                               gCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACcgcg                                                     <  1:588411/140‑1 (MQ=255)                                                                                      ggCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTcac                                              <  1:1019028/140‑1 (MQ=255)                                                                                      ggCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTcac                                              <  1:342690/140‑1 (MQ=255)                                                                                      ggCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTcac                                              <  2:365746/140‑1 (MQ=255)                                                                                      ggCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTcac                                              <  1:720875/140‑1 (MQ=255)                                                                                      ggCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTcac                                              <  1:1147968/140‑1 (MQ=255)                                                                                            aTAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCtt                                        >  2:353970/1‑140 (MQ=255)                                                                                                ttCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGcc                                    <  1:1196743/140‑1 (MQ=255)                                                                                                     caacTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGtt                               >  1:590336/1‑140 (MQ=255)                                                                                                     caacTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGtt                               >  2:1091275/1‑140 (MQ=255)                                                                                                     caacTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGtt                               >  2:1169073/1‑140 (MQ=255)                                                                                                      aacTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTc                              <  2:744717/140‑1 (MQ=255)                                                                                                      aacTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTc                              <  2:780017/140‑1 (MQ=255)                                                                                                      aacTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTc                              <  1:353970/140‑1 (MQ=255)                                                                                                      aacTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTc                              <  1:824214/140‑1 (MQ=255)                                                                                                        cTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCAc                            >  2:434247/1‑140 (MQ=255)                                                                                                          gcgcGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCa                             <  2:1162318/137‑1 (MQ=255)                                                                                                          gcgcGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCa                             >  1:1162318/1‑137 (MQ=255)                                                                                                          gcgcGTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACgg                          >  2:136133/1‑140 (MQ=255)                                                                                                           cgcgTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGATGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGc                         <  2:694682/140‑1 (MQ=255)                                                                                                              gTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCaa                      <  2:281496/140‑1 (MQ=255)                                                                                                              gTCAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCaa                      <  2:763443/140‑1 (MQ=255)                                                                                                                cAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAaga                    <  1:473327/140‑1 (MQ=255)                                                                                                                cAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAaga                    >  2:1279361/1‑140 (MQ=255)                                                                                                                cAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAaga                    >  2:378080/1‑140 (MQ=255)                                                                                                                cAGCTGTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAaga                    <  1:1169073/140‑1 (MQ=255)                                                                                                                     gTCGCAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGt               <  1:449100/140‑1 (MQ=255)                                                                                                                         cAGCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAAt           <  1:136133/140‑1 (MQ=255)                                                                                                                           gCCATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTc         <  1:1091275/140‑1 (MQ=255)                                                                                                                             cATAAAGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCAc       <  2:1078935/140‑1 (MQ=255)                                                                                                                                 aaGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGt   <  1:1279361/140‑1 (MQ=255)                                                                                                                                  aGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAATCTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTc  <  2:549010/140‑1 (MQ=255)                                                                                                                                  aGGCAAGCTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGAGGTCAATTCACCTGTc  <  1:733381/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     GAATCGGTTCTTTACCAGAAACTTTTGGCATCATAAAACCGATAACTTTTCCGCCTCGTCCACCGTGAAGCGTTGCCTGCGGCCAGGCGACATAATTCAACAACTGCGCGTCAGCTGTCGCAGCCATAAAGGCAAGTTTGTCCTGTTTTAAGGCGGGTGGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTC  >  NC_000913/2150688‑2150956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑