Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,265,666	G→A	D73N (GAC→AAC)	yeiP →	elongation factor P family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,265,666	0	G	A	100.0%	183.7 / NA	52	D73N (GAC→AAC)	yeiP	elongation factor P family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/20);  total (32/20)

TTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATGCCGGACATGCAGGTGC  >  NC_000913/2265529‑2265801                                                                                                                                          |                                                                                                                                        ttCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAAc                                                                                                                                       >  1:18926/1‑140 (MQ=255)      tCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTtctc                                                                                                                                  >  1:354509/1‑140 (MQ=255)      tCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTtctc                                                                                                                                  >  1:1275221/1‑140 (MQ=255)        gagacgggCCCGGGGCGCCGCTACGCTAAAAAAATTGTTTTTTTCTGATGCCCGTCCGGGGAAAAAAGTAGAAGGGCTTTAAAAGGGTGATGATATCGTTGCCCCGGGGCCGCTGACCCGCCGTTACGTTAACTTCTCCt                                                                                                                                <  2:1079682/133‑1 (MQ=255)        gCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCt                                                                                                                                >  1:1129583/1‑140 (MQ=255)         cccACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTa                                                                                                                               >  1:669283/1‑140 (MQ=255)              tGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCg                                                                                                                          >  1:1203204/1‑140 (MQ=255)                cccGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGAt                                                                                                                        >  2:1245768/1‑140 (MQ=255)                    gggTCCCGCTACGCTATAAAAATTGTTTTTTTTTGATGCCCGTGCGGGGAAAAAAGAAGAAGAGCTTTAAAAGGGTGATGATTTCGTTGCCCCGGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCa                                                                                                                    <  2:743396/139‑1 (MQ=255)                    cgGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCa                                                                                                                    >  1:743396/1‑140 (MQ=255)                         ccGCTACGCTATAAAAATTGTTTTTTTCTGATGTCCGTCCGGGGAAAAAAGAAGAAGAGTTTTTAAAGGGTAATTATTTCGTAGCCCCGGTGCCGCTGCCCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGaa                                                                                                               <  2:1203204/140‑1 (MQ=255)                          cGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGcctgtctc                                                                                                              >  1:1079682/1‑133 (MQ=255)                          cGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAat                                                                                                              >  1:818084/1‑140 (MQ=255)                                 cTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCtt                                                                                                       >  1:1233842/1‑140 (MQ=255)                                              gTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAaagaa                                                                                          >  2:1161692/1‑140 (MQ=255)                                                     tGATGCCCGTCCGGGGCAAAAAGTAGAAGAGCGTTTAAAGGGTGATGATTTCGTTGACACCGGGACGCTGCCCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACtata                                                                                   <  2:533901/140‑1 (MQ=255)                                                            cGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGta                                                                            >  2:475017/1‑140 (MQ=255)                                                                  gggATGAAAGTAGAGGAGTGTTAAAAGGGTGATGATGTCCTTCACTCTGCGCCTCGTCCCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCtt                                                                      <  2:548529/140‑1 (MQ=255)                                                                   ggCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTc                                                                     >  1:256729/1‑140 (MQ=255)                                                                    gCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCa                                                                    >  2:1035413/1‑140 (MQ=255)                                                                    gCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCa                                                                    >  1:625077/1‑140 (MQ=255)                                                                                agggtttttAAAAGGTGATATTATCGTGGCCGCCGGGCCGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGa                                                        <  1:642093/134‑1 (MQ=255)                                                                                  agCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGAtt                                                      >  1:1161763/1‑140 (MQ=255)                                                                                  agCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGAtt                                                      >  2:1276470/1‑140 (MQ=255)                                                                                    cGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTga                                                    >  2:1250528/1‑140 (MQ=255)                                                                                       ttCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTgaaga                                                 >  1:1097397/1‑140 (MQ=255)                                                                                              gtaatgatACCGTTGCCACGGTGACGCAACCCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTg                                          <  1:412131/137‑1 (MQ=255)                                                                                              gTGATAATTTGGTTGACACGGTGACGCTGCCCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTg                                          <  2:1053243/140‑1 (MQ=255)                                                                                                 atgatATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTg                                       >  2:1242649/1‑140 (MQ=255)                                                                                                 atgatATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTg                                       >  1:783196/1‑140 (MQ=255)                                                                                                 atgatATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTg                                       >  2:1137011/1‑140 (MQ=255)                                                                                                 atgatATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGCTgtct                                       >  1:1056677/1‑137 (MQ=255)                                                                                                  tgatTTCTTCGACCCGGTGCCGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGt                                      <  2:625077/140‑1 (MQ=255)                                                                                                           tGACACCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGCCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCag                             >  2:728267/1‑140 (MQ=255)                                                                                                               acCGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAggg                         >  2:374500/1‑140 (MQ=255)                                                                                                                 cGTGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGggcg                       >  1:233865/1‑140 (MQ=255)                                                                                                                   tGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGggcggc                     >  1:1015893/1‑140 (MQ=255)                                                                                                                   tGACGCTGACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGggcggc                     >  2:632460/1‑140 (MQ=255)                                                                                                                     ccGCTCACCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCAt                   <  2:507102/139‑1 (MQ=255)                                                                                                                           ccccGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATGCCGGa             <  1:1177824/139‑1 (MQ=255)                                                                                                                           aCCCGCCGTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATACCGGa             >  2:52137/1‑140 (MQ=255)                                                                                                                               gccgTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATGCCGGACATg         <  1:52137/140‑1 (MQ=255)                                                                                                                                ccgTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATGCCGGACATGc        >  1:455406/1‑140 (MQ=255)                                                                                                                                ccgTTACGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATGCCGGACATGc        <  2:354509/140‑1 (MQ=255)                                                                                                                                     aCGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATGCCGGACATGCAGGTg   <  2:527453/140‑1 (MQ=255)                                                                                                                                     aCGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATGCCGGACATGCAGGTg   >  2:910812/1‑140 (MQ=255)                                                                                                                                     aCGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATGCCGGACATGCAGGTg   <  2:818084/140‑1 (MQ=255)                                                                                                                                     aCGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATGCCGGACATGCAGGTg   <  2:783196/140‑1 (MQ=255)                                                                                                                                     aCGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATGCCGGACATGCAGGTg   <  1:1137011/140‑1 (MQ=255)                                                                                                                                     aCGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATGCCGGACATGCAGGTg   <  2:455406/140‑1 (MQ=255)                                                                                                                                     aCGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATGCCGGACATGCAGGTg   <  1:374500/140‑1 (MQ=255)                                                                                                                                      cGTTAACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATGCCGGACATGCAGGTGc  <  1:475017/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCCGTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGATATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGTTTATGCCAGAGGGCGGCATGCCGGACATGCAGGTGC  >  NC_000913/2265529‑2265801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑