Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,273,984	C→T	R541* (CGA→TGA)	yejA →	putative oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,273,984	0	C	T	100.0%	225.0 / NA	65	R541* (CGA→TGA)	yejA	putative oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/38);  total (27/38)

ATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGCGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACC  >  NC_000913/2273847‑2274118                                                                                                                                          |                                                                                                                                       aTTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGa                                                                                                                                      >  1:42307/1‑140 (MQ=255)  tttACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAg                                                                                                                                     <  1:947561/140‑1 (MQ=255)         aTTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGa                                                                                                                              <  2:905963/140‑1 (MQ=255)         aTTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGa                                                                                                                              <  1:40190/140‑1 (MQ=255)         aTTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGa                                                                                                                              <  2:196130/140‑1 (MQ=255)           ttCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATc                                                                                                                            <  2:298642/140‑1 (MQ=255)            tCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATcg                                                                                                                           <  1:1074045/140‑1 (MQ=255)                ggTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTa                                                                                                                       <  1:1259369/140‑1 (MQ=255)                       ggAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGt                                                                                                                <  2:816707/140‑1 (MQ=255)                        gAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTg                                                                                                               <  1:801666/140‑1 (MQ=255)                           tatatCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGTTGAGCACTGGATCGCGTATTAACGTGGaa                                                                                                            <  1:20184/140‑1 (MQ=255)                                   ttCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACa                                                                                                    >  1:641479/1‑140 (MQ=255)                                   ttCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACa                                                                                                    <  2:352733/140‑1 (MQ=255)                                   ttCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACa                                                                                                    >  2:1049056/1‑140 (MQ=255)                                   ttCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACa                                                                                                    >  1:1212444/1‑140 (MQ=255)                                   ttCCACTTATAATGCCCCCGGCGTACAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACa                                                                                                    >  1:975546/1‑140 (MQ=255)                                    tCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACAt                                                                                                   >  2:773890/1‑140 (MQ=255)                                        cTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACAtgctg                                                                                               >  2:1065858/1‑140 (MQ=255)                                         ttATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACAtgctgc                                                                                              <  1:72397/140‑1 (MQ=255)                                         ttATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACAtgctgc                                                                                              <  1:1049056/140‑1 (MQ=255)                                           ataATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCa                                                                                            <  2:1236969/140‑1 (MQ=255)                                           ataATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCa                                                                                            <  1:644309/140‑1 (MQ=255)                                           ataATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCa                                                                                            <  1:862961/140‑1 (MQ=255)                                             aaTGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAAt                                                                                          >  1:370681/1‑140 (MQ=255)                                             aaTGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAAt                                                                                          <  1:1102658/140‑1 (MQ=255)                                                gCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCa                                                                                            >  2:1058733/1‑135 (MQ=255)                                                gCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCa                                                                                            <  1:1058733/135‑1 (MQ=255)                                                 cccccGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTgg                                                                                      >  1:477826/1‑140 (MQ=255)                                                   cccGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTa                                                                                    >  1:620248/1‑140 (MQ=255)                                                   cccGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTa                                                                                    >  1:1229820/1‑140 (MQ=255)                                                      ggCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACAtgctgc                                                                                              >  2:746735/1‑127 (MQ=255)                                                      ggCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACAtgctgc                                                                                              <  1:746735/127‑1 (MQ=255)                                                          tGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCg                                                                             >  1:855887/1‑140 (MQ=255)                                                           gCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCgg                                                                            >  1:370609/1‑140 (MQ=255)                                                            cAAAGCCCGGGTATCAACTCGCTGATTAACCAAATTCTTGCCGCGCAGGGCAATAAAGAAAAATAACTGCCGTTGGCGCTAGCACTGGATCGCGTATTAACGACGAATTATTACATGCAGCCAATGTCGTACATGGCCGa                                                                           >  2:691583/1‑140 (MQ=255)                                                                 cccGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGAcc                                                                      <  1:675036/140‑1 (MQ=255)                                                                    ggTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGtc                                                                   >  2:1150374/1‑140 (MQ=255)                                                                        aTCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGc                                                               <  2:975546/140‑1 (MQ=255)                                                                        aTCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGc                                                               <  1:773287/140‑1 (MQ=255)                                                                           gACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGATGACCGTCTCGCCtg                                                            >  2:787247/1‑140 (MQ=255)                                                                           gACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCtg                                                            <  2:641479/140‑1 (MQ=255)                                                                                gCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTggg                                                       >  1:195161/1‑140 (MQ=255)                                                                                gCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTggg                                                       >  2:1178220/1‑140 (MQ=255)                                                                                    aTCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATaa                                                   >  1:1033429/1‑140 (MQ=255)                                                                                     tCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATaaa                                                  <  1:1178220/140‑1 (MQ=255)                                                                                                       cgcAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTg                                >  1:840867/1‑140 (MQ=255)                                                                                                       cgcAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTg                                <  2:659821/140‑1 (MQ=255)                                                                                                       cgcAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTg                                >  1:239163/1‑140 (MQ=255)                                                                                                       cgcAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTg                                >  2:1242236/1‑140 (MQ=255)                                                                                                        gcAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTgc                               >  2:553128/1‑140 (MQ=255)                                                                                                         cAGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTgcg                              >  1:954638/1‑140 (MQ=255)                                                                                                          aGGGAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTgcgc                             <  2:195161/140‑1 (MQ=255)                                                                                                            ggAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGccc                           <  2:370609/140‑1 (MQ=255)                                                                                                            ggAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGccc                           <  2:1212444/140‑1 (MQ=255)                                                                                                            ggAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGccc                           <  1:787247/140‑1 (MQ=255)                                                                                                            ggAAATAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGccc                           <  1:828716/140‑1 (MQ=255)                                                                                                               aaTAAAGAAAAATTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCAt                        <  1:830529/140‑1 (MQ=255)                                                                                                                      aaaaaTTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGc                 >  2:58184/1‑140 (MQ=255)                                                                                                                          aTTACTGCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCg             <  1:133293/140‑1 (MQ=255)                                                                                                                                gCCGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCg       <  2:477826/140‑1 (MQ=255)                                                                                                                                 ccGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGa      <  2:620248/140‑1 (MQ=255)                                                                                                                                  cGTTGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGAt     <  1:515424/140‑1 (MQ=255)                                                                                                                                    ttGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATAc   >  2:979294/1‑140 (MQ=255)                                                                                                                                    ttGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATAc   >  1:397444/1‑140 (MQ=255)                                                                                                                                     tGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATAcc  <  1:979294/140‑1 (MQ=255)                                                                                                                                     tGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATAcc  <  1:1150374/140‑1 (MQ=255)                                                                                                                                     tGGGGTGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATAcc  <  2:1033429/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       ATTTACAGATTTCCTGGTCATCGGAATATATCAATTCCACTTATAATGCCCCCGGCGTGCAAAGCCCGGTTATCGACTCGCTGATCAACCAAATTATTGCCGCGCAGGGAAATAAAGAAAAATTACTGCCGTTGGGGCGAGCACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACC  >  NC_000913/2273847‑2274118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑