Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,300,312	T→C	K814K (AAA→AAG)	napA ←	periplasmic nitrate reductase subunit NapA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,300,312	0	T	C	100.0%	238.2 / NA	64	K814K (AAA→AAG)	napA	periplasmic nitrate reductase subunit NapA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/36);  total (28/36)

CCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGTTTCAACAATCGAGATCACTTCGCCACGG  >  NC_000913/2300176‑2300447                                                                                                                                         |                                                                                                                                        ccACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTc                                                                                                                                      <  1:1101861/140‑1 (MQ=255)    cGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCgag                                                                                                                                   >  2:1823/1‑140 (MQ=255)          ccagccCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGa                                                                                                                             >  1:1124540/1‑140 (MQ=255)           cagccCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATGATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGAt                                                                                                                            <  1:383858/140‑1 (MQ=255)           cagccCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGAt                                                                                                                            <  2:350178/140‑1 (MQ=255)           cagccCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGAt                                                                                                                            >  2:21495/1‑140 (MQ=255)               ccGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGt                                                                                                                        >  2:882198/1‑140 (MQ=255)               ccGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGt                                                                                                                        <  1:21495/140‑1 (MQ=255)                 gCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCg                                                                                                                      <  2:676004/140‑1 (MQ=255)                        gtgCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAg                                                                                                               >  1:101068/1‑140 (MQ=255)                           cGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGt                                                                                                            >  1:726536/1‑140 (MQ=255)                            gAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTc                                                                                                           <  2:1124540/140‑1 (MQ=255)                             aaCGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCa                                                                                                          <  2:180395/140‑1 (MQ=255)                               cGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGt                                                                                                        <  2:101068/140‑1 (MQ=255)                                gACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGtt                                                                                                       >  2:98669/1‑140 (MQ=255)                                gACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGtt                                                                                                       <  2:1276509/140‑1 (MQ=255)                                gACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGtt                                                                                                       <  1:317980/140‑1 (MQ=255)                                   aTCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGt                                                                                                    >  2:354118/1‑140 (MQ=255)                                     cgcgCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTa                                                                                                  >  2:803520/1‑140 (MQ=255)                                     cgcgCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTa                                                                                                  >  2:388593/1‑140 (MQ=255)                                       cgcAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAAc                                                                                                <  2:104002/140‑1 (MQ=255)                                          aGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCaa                                                                                             >  2:512542/1‑140 (MQ=255)                                               gcggcggcgACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACtgtg                                                                                        <  1:354118/140‑1 (MQ=255)                                                  gcggcgACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTgcgg                                                                                     <  2:576306/140‑1 (MQ=255)                                                           aTTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGGGGCGTCgaaga                                                                            <  1:301646/140‑1 (MQ=255)                                                             tttGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAAc                                                                          <  1:882350/140‑1 (MQ=255)                                                             tttGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAAc                                                                          <  2:726536/140‑1 (MQ=255)                                                             tttGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAAc                                                                          <  1:98669/140‑1 (MQ=255)                                                              ttGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACg                                                                         <  1:388593/140‑1 (MQ=255)                                                                  ggTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCAt                                                                     <  2:166880/140‑1 (MQ=255)                                                                         cTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACAcc                                                              <  1:1823/140‑1 (MQ=255)                                                                                ggACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACAcc                                                              <  2:752763/133‑1 (MQ=255)                                                                                ggACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACAcc                                                              >  1:752763/1‑133 (MQ=255)                                                                                    aTAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTgg                                                   <  2:316229/140‑1 (MQ=255)                                                                                      aaTGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCg                                                 >  2:363446/1‑140 (MQ=255)                                                                                       aTGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCgg                                                <  2:567755/140‑1 (MQ=255)                                                                                       aTGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCgg                                                <  2:621526/140‑1 (MQ=255)                                                                                       aTGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCgg                                                <  1:842761/140‑1 (MQ=255)                                                                                       aTGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCgg                                                <  2:183666/140‑1 (MQ=255)                                                                                           cTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGa                                               >  2:463566/1‑137 (MQ=255)                                                                                           cTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGa                                               <  1:463566/137‑1 (MQ=255)                                                                                           cTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACgg                                            >  1:1184228/1‑140 (MQ=255)                                                                                           cTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACgg                                            >  1:293395/1‑140 (MQ=255)                                                                                            ttACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCgg                                                >  1:963869/1‑135 (MQ=255)                                                                                            ttACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCgg                                                <  2:963869/135‑1 (MQ=255)                                                                                            ttACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGt                                           >  1:649256/1‑140 (MQ=255)                                                                                            ttACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGt                                           <  1:882198/140‑1 (MQ=255)                                                                                                 ccTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGc                                                  <  2:969176/128‑1 (MQ=255)                                                                                                 ccTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGc                                                  >  1:969176/1‑128 (MQ=255)                                                                                                    tctcCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACcg                                   >  2:888591/1‑140 (MQ=255)                                                                                                    tctcCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACcg                                   <  1:888591/140‑1 (MQ=255)                                                                                                               aCCGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGTTTcaac                        >  2:1176569/1‑140 (MQ=255)                                                                                                                ccGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGTTTcaaca                       <  2:649256/140‑1 (MQ=255)                                                                                                                ccGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGTTTcaaca                       <  1:175465/140‑1 (MQ=255)                                                                                                                ccGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGTTTcaaca                       <  1:371360/140‑1 (MQ=255)                                                                                                                ccGCGCACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGTTTcaaca                       <  2:383215/140‑1 (MQ=255)                                                                                                                  gcgcACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACTGCGCGTTTCAACAAt                     <  2:500255/140‑1 (MQ=255)                                                                                                                     cACTTCTTGAAGTCCGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGTTTCAACAATCga                  >  1:762954/1‑140 (MQ=255)                                                                                                                                  ccGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGTTTCAACAATCGAGATCACTTCGCCa     >  1:98473/1‑140 (MQ=255)                                                                                                                                  ccGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGTTTCAACAATCGAGATCACTTCGCCa     >  2:1062875/1‑140 (MQ=255)                                                                                                                                   cGTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTAGCGGACCGTTACGTCCGCGCGGTTCCACCATCGAGATCCCTTCCCCAc    >  2:861487/1‑140 (MQ=255)                                                                                                                                    gTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGTTTCAACAATCGAGATCACTTCGCCACg   >  1:1024199/1‑140 (MQ=255)                                                                                                                                    gTCTCCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGTTTCAACAATCGAGATCACTTCGCCACg   >  1:767340/1‑140 (MQ=255)                                                                                                                                     tctcCTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGTGGACGGTTACGACCGCGCGTTTCAACAATCGAGATCACTTCGCCACgg  >  2:128426/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGTTTCAACAATCGAGATCACTTCGCCACGG  >  NC_000913/2300176‑2300447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑