Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,321,258	G→A	A406T (GCT→ACT)	atoS →	sensory histidine kinase AtoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,321,258	0	G	A	100.0%	320.7 / NA	90	A406T (GCT→ACT)	atoS	sensory histidine kinase AtoS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/55);  total (35/55)

TAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTC  >  NC_000913/2321124‑2321390                                                                                                                                       |                                                                                                                                     tAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGTTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTAtt                                                                                                                                 >  2:639341/1‑140 (MQ=255)   ggTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCg                                                                                                                               <  1:1036821/140‑1 (MQ=255)   ggTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCg                                                                                                                               >  2:1093358/1‑140 (MQ=255)   ggTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCg                                                                                                                               >  1:1269415/1‑140 (MQ=255)   ggTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCg                                                                                                                               <  1:1261784/140‑1 (MQ=255)   ggTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCg                                                                                                                               <  1:655620/140‑1 (MQ=255)   ggTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCg                                                                                                                               <  2:744438/140‑1 (MQ=255)   ggTGCTTTGGTGATTTTCTCTGATTTAACTACCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCg                                                                                                                               <  2:786062/140‑1 (MQ=255)     tGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCg                                                                                                                               <  1:340529/138‑1 (MQ=255)     tGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCg                                                                                                                               >  2:340529/1‑138 (MQ=255)     tGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTg                                                                                                                             >  2:362052/1‑140 (MQ=255)     tGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTg                                                                                                                             >  2:61083/1‑140 (MQ=255)     tGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTg                                                                                                                             >  2:1130975/1‑140 (MQ=255)     tGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTg                                                                                                                             <  1:362052/140‑1 (MQ=255)     tGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTg                                                                                                                             <  2:665291/140‑1 (MQ=255)      gCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTgg                                                                                                                            <  2:928765/140‑1 (MQ=255)        tttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCTTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          <  1:1070784/140‑1 (MQ=255)        tttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGTTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          <  1:639341/140‑1 (MQ=255)        tttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          <  1:640159/140‑1 (MQ=255)        tttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          <  1:61083/140‑1 (MQ=255)        tttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          <  1:983011/140‑1 (MQ=255)        tttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          <  2:1000875/140‑1 (MQ=255)        tttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          <  1:883615/140‑1 (MQ=255)        tttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          >  2:687468/1‑140 (MQ=255)        tttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          <  1:324442/140‑1 (MQ=255)        tttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          <  2:874257/140‑1 (MQ=255)        tttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          <  2:81107/140‑1 (MQ=255)        tttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          >  1:1200725/1‑140 (MQ=255)        tttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          <  1:1130975/140‑1 (MQ=255)         ttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          <  1:854882/139‑1 (MQ=255)         ttGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGtt                                                                                                                          >  2:854882/1‑139 (MQ=255)             tGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTa                                                                                                                     <  1:1104468/140‑1 (MQ=255)               aTTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACa                                                                                                                   >  2:70832/1‑140 (MQ=255)               aTTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACa                                                                                                                   >  1:231165/1‑140 (MQ=255)               aTTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACa                                                                                                                   >  1:238157/1‑140 (MQ=255)                       tGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTgc                                                                                                           <  1:1105420/140‑1 (MQ=255)                              aCTGCCCGCATAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCCTGCGCcaaca                                                                                                    <  1:1193285/140‑1 (MQ=255)                              aCTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCcaaca                                                                                                    >  1:623380/1‑140 (MQ=255)                               cTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCcaacaa                                                                                                   >  2:1070857/1‑140 (MQ=255)                                   ccGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCa                                                                                               <  2:238157/140‑1 (MQ=255)                                   ccGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCa                                                                                               >  2:934331/1‑140 (MQ=255)                                    cGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAg                                                                                              <  1:101908/140‑1 (MQ=255)                                     gCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGt                                                                                             <  1:988870/140‑1 (MQ=255)                                       aaaGAAGCCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGa                                                                                           <  2:134913/140‑1 (MQ=255)                                         aGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGAcc                                                                                         <  1:755235/140‑1 (MQ=255)                                          gAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGAccc                                                                                        <  2:259163/140‑1 (MQ=255)                                          gAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGAccc                                                                                        <  2:14811/140‑1 (MQ=255)                                           aaaCCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCa                                                                                       <  2:1099169/140‑1 (MQ=255)                                            aaCCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCaa                                                                                      <  2:582919/140‑1 (MQ=255)                                            aaCCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCaa                                                                                      <  1:511501/140‑1 (MQ=255)                                            aaCCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCaa                                                                                      <  2:324001/140‑1 (MQ=255)                                            aaCCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCaa                                                                                      <  1:348287/140‑1 (MQ=255)                                               ccAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCAGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATAc                                                                                   >  1:149305/1‑140 (MQ=255)                                                cAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACa                                                                                  <  2:231165/140‑1 (MQ=255)                                                cAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACa                                                                                  <  2:627277/140‑1 (MQ=255)                                                 aGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACAt                                                                                 <  2:32144/140‑1 (MQ=255)                                                  gcgcCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATc                                                                                <  2:1200725/140‑1 (MQ=255)                                                  gcgcCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATc                                                                                <  1:1043114/140‑1 (MQ=255)                                                   cgccgcATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGa                                                                                           >  2:774529/1‑128 (MQ=255)                                                   cgccgcATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGa                                                                                           <  1:774529/128‑1 (MQ=255)                                                     ccgcATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAgg                                                                             <  2:623380/140‑1 (MQ=255)                                                             cgcAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCGGGAATATCtt                                                                     >  2:24453/1‑139 (MQ=255)                                                              gcAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGt                                                                    <  2:211830/140‑1 (MQ=255)                                                              gcAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGt                                                                    <  1:934331/140‑1 (MQ=255)                                                                   cAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCgta                                                               <  1:1070857/140‑1 (MQ=255)                                                                     gAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCgtagt                                                             >  2:1159176/1‑140 (MQ=255)                                                                             cGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAg                                                     >  1:1026280/1‑140 (MQ=255)                                                                                       ggTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTc                                           >  2:937958/1‑140 (MQ=255)                                                                                       ggTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTc                                           <  1:70832/140‑1 (MQ=255)                                                                                        gTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCa                                          <  1:687468/140‑1 (MQ=255)                                                                                             cTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTaa                                     <  1:937958/140‑1 (MQ=255)                                                                                             cTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTaa                                     >  2:460792/1‑140 (MQ=255)                                                                                              tGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAAc                                    >  2:275634/1‑140 (MQ=255)                                                                                               gATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACa                                   >  1:837600/1‑140 (MQ=255)                                                                                                aTGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACaa                                  <  2:1269415/140‑1 (MQ=255)                                                                                                aTGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACaa                                  <  1:275634/140‑1 (MQ=255)                                                                                                 tggctggcGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACaaa                                 >  1:480621/1‑140 (MQ=255)                                                                                                 tggctggcGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACaaa                                 >  2:707362/1‑140 (MQ=255)                                                                                                 tggctggcGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACaaa                                 >  1:822305/1‑140 (MQ=255)                                                                                                 tggctggcGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACaaa                                 >  1:690065/1‑140 (MQ=255)                                                                                                  ggctggcGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAg                                <  1:435774/140‑1 (MQ=255)                                                                                                  ggctggcGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAg                                <  2:837600/140‑1 (MQ=255)                                                                                                  ggctggcGTCGCGCATGAAGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAg                                >  2:435774/1‑140 (MQ=255)                                                                                                                   aaGTACGTAATCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTc               <  2:690065/140‑1 (MQ=255)                                                                                                                             tCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCAc     >  2:739308/1‑140 (MQ=255)                                                                                                                             tCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCAc     >  2:1220477/1‑140 (MQ=255)                                                                                                                             tCCGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCAc     >  1:539732/1‑140 (MQ=255)                                                                                                                               cGTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGt   <  1:1005485/140‑1 (MQ=255)                                                                                                                                gTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTc  >  2:823360/1‑140 (MQ=255)                                                                                                                                gTTAACGACTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTc  >  1:391325/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     TAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTTATTCAGCAATTGCTCGAATTTTCACGTC  >  NC_000913/2321124‑2321390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑