Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,388,965	G→A	D111N (GAT→AAT)	yfbO →	uncharacterized protein YfbO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,388,965	0	G	A	100.0%	232.1 / NA	64	D111N (GAT→AAT)	yfbO	uncharacterized protein YfbO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/42);  total (22/42)

TAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACAT  >  NC_000913/2388829‑2389092                                                                                                                                         |                                                                                                                                tAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCtaatg                                                                                                                              >  2:1207242/1‑140 (MQ=255)  aaCAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCtaatga                                                                                                                             <  1:271681/140‑1 (MQ=255)   aCAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAg                                                                                                                            <  2:1076710/140‑1 (MQ=255)   aCAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAg                                                                                                                            <  1:174500/140‑1 (MQ=255)     aaTGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCGCCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTg                                                                                                                          <  2:840906/140‑1 (MQ=255)     aaTGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTg                                                                                                                          <  1:1017317/140‑1 (MQ=255)     aaTGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTg                                                                                                                          <  2:1166302/140‑1 (MQ=255)       tGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCtaat                                                                                                                               >  1:1210127/1‑133 (MQ=255)       tGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCtaat                                                                                                                               >  1:1255440/1‑133 (MQ=255)           aGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGa                                                                                                                    >  1:695879/1‑140 (MQ=255)           aGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGa                                                                                                                    >  2:680025/1‑140 (MQ=255)            gTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGaa                                                                                                                   >  2:714889/1‑140 (MQ=255)              gATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAgg                                                                                                                 <  1:66184/140‑1 (MQ=255)              gATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAgg                                                                                                                 <  1:435290/140‑1 (MQ=255)              gATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAgg                                                                                                                 <  1:380900/140‑1 (MQ=255)               aTGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGa                                                                                                                >  2:2959/1‑140 (MQ=255)               aTGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGa                                                                                                                >  2:1022325/1‑140 (MQ=255)                   tttGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGt                                                                                                            <  2:506435/140‑1 (MQ=255)                   tttGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGt                                                                                                            >  1:506435/1‑140 (MQ=255)                    ttGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTc                                                                                                           <  2:1128286/140‑1 (MQ=255)                    ttGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTc                                                                                                           <  1:357367/140‑1 (MQ=255)                       gTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCaga                                                                                                        <  1:287360/140‑1 (MQ=255)                            aaTTACGATGTTCGATGACCCAGACTGGCCCTTTATTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATa                                                                                                   <  1:1246366/140‑1 (MQ=255)                            aaTTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATa                                                                                                   <  2:658473/140‑1 (MQ=255)                            aaTTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATa                                                                                                   <  2:1124443/140‑1 (MQ=255)                            aaTTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATa                                                                                                   <  1:1015828/140‑1 (MQ=255)                            aaTTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATa                                                                                                   >  1:3029/1‑140 (MQ=255)                            aaTTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATa                                                                                                   >  1:1049930/1‑140 (MQ=255)                            aaTTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGAGCTGGGAAGGATGGTCAGAAGATa                                                                                                   <  1:1254731/140‑1 (MQ=255)                                 cGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAg                                                                                              <  1:13651/140‑1 (MQ=255)                                   aTGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCa                                                                                            <  1:536093/140‑1 (MQ=255)                                      ttCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCatgg                                                                                         <  1:714889/140‑1 (MQ=255)                                        cGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCatggat                                                                                       <  2:713847/140‑1 (MQ=255)                                              cccAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTg                                                                                 <  1:71515/140‑1 (MQ=255)                                                cAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGaa                                                                               <  1:1022325/140‑1 (MQ=255)                                                cAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGaa                                                                               >  2:89207/1‑140 (MQ=255)                                                     tGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTccc                                                                          <  2:800126/140‑1 (MQ=255)                                                       gCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCtg                                                                        >  2:421635/1‑140 (MQ=255)                                                       gCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGACCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCtg                                                                        >  2:296392/1‑140 (MQ=255)                                                        cccTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCtgt                                                                       <  1:89207/140‑1 (MQ=255)                                                        cccTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCtgt                                                                       <  1:263185/140‑1 (MQ=255)                                                        cccTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCtgt                                                                       >  2:1160217/1‑140 (MQ=255)                                                           tttAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTggg                                                                    <  2:1200740/140‑1 (MQ=255)                                                                  aaaGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCAt                                                             <  1:680025/140‑1 (MQ=255)                                                                  aaaGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCAt                                                             <  2:3029/140‑1 (MQ=255)                                                                  aaaGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCAt                                                             <  2:1049930/140‑1 (MQ=255)                                                                                  caccacTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCTGGTAACTTGTtggt                                             >  2:788520/1‑140 (MQ=255)                                                                                   accacTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTtggtg                                            <  2:1248010/140‑1 (MQ=255)                                                                                         gCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCATAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTa                                      >  1:1198514/1‑140 (MQ=255)                                                                                          cTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTaa                                     >  1:805286/1‑140 (MQ=255)                                                                                          cTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTaa                                     <  1:1160217/140‑1 (MQ=255)                                                                                          cTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTaa                                     <  1:1207242/140‑1 (MQ=255)                                                                                              aCCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACa                                 <  1:2959/140‑1 (MQ=255)                                                                                              aCCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTATGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACa                                 <  2:79047/140‑1 (MQ=255)                                                                                               ccAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACAt                                >  2:135933/1‑140 (MQ=255)                                                                                                     gaagTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTtatat                          <  1:273569/140‑1 (MQ=255)                                                                                                      aagTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTtatata                         <  1:437085/140‑1 (MQ=255)                                                                                                      aagTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTCGGTGGGATTAAAACATTtatata                         <  1:1224390/140‑1 (MQ=255)                                                                                                           cAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGATGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAg                    <  2:802866/140‑1 (MQ=255)                                                                                                           cAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACa                                 >  2:436550/1‑127 (MQ=255)                                                                                                           cAGAGCTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACa                                 <  1:436550/127‑1 (MQ=255)                                                                                                               gCTGGTTTGTAGAAGAAATTGCCCCCAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGAt                  <  2:440560/138‑1 (MQ=255)                                                                                                               gCTGGTTTGTAGAAGAAATTGCCCCCAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGAt                  >  1:440560/1‑138 (MQ=255)                                                                                                                cTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAg               >  1:1137021/1‑140 (MQ=255)                                                                                                                cTGGTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAg               >  1:495489/1‑140 (MQ=255)                                                                                                                   gTTTGTAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCgg            >  1:548138/1‑140 (MQ=255)                                                                                                                        tAGAAGAAATTGCCCCTAATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGtt       >  2:911366/1‑140 (MQ=255)                                                                                                                             gaaATTGCCCCTAATGAGTGCTGGGAAGGATGGTCATAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACAt  <  2:1198514/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                TAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCATCCGGTTACTTGTTGGTGGGATTAAAACATTTATATAAACAGATAAGCGGATGTTTACAT  >  NC_000913/2388829‑2389092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑