Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,457,339	T→C	T607T (ACA→ACG)	fadJ ←	3‑hydroxyacyl‑CoA dehydrogenase FadJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,457,339	0	T	C	100.0%	192.1 / NA	52	T607T (ACA→ACG)	fadJ	3‑hydroxyacyl‑CoA dehydrogenase FadJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/22);  total (30/22)

CGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTT  >  NC_000913/2457213‑2457471                                                                                                                               |                                                                                                                                     cgcgCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCg                                                                                                                         >  2:198660/1‑140 (MQ=255)   cgcCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCggg                                                                                                                       >  2:198354/1‑140 (MQ=255)     ccAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTa                                                                                                                     <  1:1169766/140‑1 (MQ=255)        atatCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAAt                                                                                                                  >  1:891141/1‑140 (MQ=255)          atCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATgg                                                                                                                <  1:547247/140‑1 (MQ=255)           tCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGc                                                                                                               >  2:71732/1‑140 (MQ=255)              ccGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCggg                                                                                                            <  1:437303/140‑1 (MQ=255)               cGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGa                                                                                                           <  2:1186919/140‑1 (MQ=255)               cGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGa                                                                                                           <  1:369670/140‑1 (MQ=255)                gTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGAt                                                                                                          >  2:227248/1‑140 (MQ=255)                  cacgcacgCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCg                                                                                                        >  1:184910/1‑140 (MQ=255)                           tACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGtttt                                                                                               >  1:109333/1‑140 (MQ=255)                              ggATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTg                                                                                            <  1:227248/140‑1 (MQ=255)                               gATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGc                                                                                           >  2:13569/1‑140 (MQ=255)                                  aaCCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCttt                                                                                        <  2:891141/140‑1 (MQ=255)                                   aCCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCtttt                                                                                       >  2:585121/1‑140 (MQ=255)                                   aCCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCtttt                                                                                       >  1:194905/1‑140 (MQ=255)                                      tGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACCCACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACg                                                                                    <  1:877688/140‑1 (MQ=255)                                      tGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACg                                                                                    <  1:933364/140‑1 (MQ=255)                                       gCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGc                                                                                   >  1:744965/1‑140 (MQ=255)                                       gCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGc                                                                                   >  1:432911/1‑140 (MQ=255)                                         tcatcaACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGccc                                                                                 >  2:14082/1‑140 (MQ=255)                                               acacAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTg                                                                           <  1:14082/140‑1 (MQ=255)                                                     cGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATa                                                                     <  2:432911/140‑1 (MQ=255)                                                     cGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATa                                                                     <  2:109333/140‑1 (MQ=255)                                                     cGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATa                                                                     >  1:1142993/1‑140 (MQ=255)                                                     cGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATa                                                                     <  1:850847/140‑1 (MQ=255)                                                     cGTACTGCTTCATTCAGCATCAACATCACACACCGCTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATa                                                                     <  2:759004/140‑1 (MQ=255)                                                       tACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATaaa                                                                   <  2:1142993/140‑1 (MQ=255)                                                          tGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGAt                                                                >  2:204735/1‑140 (MQ=255)                                                               cattcaGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATaaa                                                                   >  2:461722/1‑132 (MQ=255)                                                               cattcaGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATaaa                                                                   <  1:461722/132‑1 (MQ=255)                                                               cattcaGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGaaa                                                           >  1:929485/1‑140 (MQ=255)                                                               cattcaGCATCAACATCACACACCGATCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCAAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGaaa                                                           >  2:949176/1‑140 (MQ=255)                                                                    aGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCg                                                      <  2:194905/140‑1 (MQ=255)                                                                      cATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGc                                                    >  1:1050009/1‑140 (MQ=255)                                                                      cATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGc                                                    >  2:661304/1‑140 (MQ=255)                                                                               cacacaCCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTc                                           <  1:204735/140‑1 (MQ=255)                                                                                    cccGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCt                                      <  2:1219031/139‑1 (MQ=255)                                                                                    acCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCt                                      >  1:1242211/1‑140 (MQ=255)                                                                                      cGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCttt                                    >  1:376646/1‑140 (MQ=255)                                                                                      cGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCttt                                    <  2:929485/140‑1 (MQ=255)                                                                                       gTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTg                                   >  1:557113/1‑140 (MQ=255)                                                                                        ttCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGc                                  >  1:4968/1‑140 (MQ=255)                                                                                        ttCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGc                                  >  2:241951/1‑140 (MQ=255)                                                                                        ttCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGc                                  >  2:610244/1‑140 (MQ=255)                                                                                        ttCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGc                                  >  2:623267/1‑140 (MQ=255)                                                                                                ccTGCGGTGCGGAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGAtcgtcg                          >  2:137943/1‑140 (MQ=255)                                                                                                  tgcggtgcggAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGtt                        >  1:87807/1‑140 (MQ=255)                                                                                                   gcggtgcggAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCTTTTGCGATCGTCGTTc                       >  2:1148391/1‑140 (MQ=255)                                                                                                          ggAGATTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTg                <  2:376646/140‑1 (MQ=255)                                                                                                            agaTTCGCCCCTGCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTgaa              <  2:1050009/140‑1 (MQ=255)                                                                                                                        gCCCTTGCGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACAttt  >  2:402020/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                     CGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTT  >  NC_000913/2457213‑2457471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑