Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,482,013	C→T	G55D (GGC→GAC)	emrY ←	tripartite efflux pump membrane subunit EmrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,482,013	0	C	T	100.0%	324.9 / NA	91	G55D (GGC→GAC)	emrY	tripartite efflux pump membrane subunit EmrY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (52/39);  total (52/39)

TACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAACGTCCCACCGGTTAATG  >  NC_000913/2481876‑2482148                                                                                                                                          |                                                                                                                                        tACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCt                                                                                                                                       >  1:956740/1‑140 (MQ=255) tACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCt                                                                                                                                       >  2:108533/1‑140 (MQ=255) tACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCt                                                                                                                                       >  1:490125/1‑140 (MQ=255)   cacaTTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTc                                                                                                                                     <  1:1068267/140‑1 (MQ=255)   cacaTTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTc                                                                                                                                     <  1:372193/140‑1 (MQ=255)      aTTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTc                                                                                                                                     <  2:405768/137‑1 (MQ=255)      aTTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTc                                                                                                                                     >  1:405768/1‑137 (MQ=255)      aTTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTc                                                                                                                                  <  1:817722/140‑1 (MQ=255)         aaTGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGt                                                                                                                               >  1:882482/1‑140 (MQ=255)           tGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCg                                                                                                                                    <  2:955228/133‑1 (MQ=255)           tGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCg                                                                                                                                    >  1:955228/1‑133 (MQ=255)           tGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTg                                                                                                                             <  1:67641/140‑1 (MQ=255)              aGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATg                                                                                                                          <  2:767058/140‑1 (MQ=255)               gACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGc                                                                                                                         <  2:882482/140‑1 (MQ=255)                aCAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCt                                                                                                                        >  1:656929/1‑140 (MQ=255)                aCAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCt                                                                                                                        >  1:47876/1‑140 (MQ=255)                  aGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTcc                                                                                                                      >  1:375372/1‑140 (MQ=255)                  aGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTcc                                                                                                                      >  1:938999/1‑140 (MQ=255)                    cGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCa                                                                                                                    >  1:787743/1‑140 (MQ=255)                    cGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCa                                                                                                                    <  1:780894/140‑1 (MQ=255)                    cGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCa                                                                                                                    >  1:1121113/1‑140 (MQ=255)                    cGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCa                                                                                                                    >  2:205834/1‑140 (MQ=255)                    cGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCa                                                                                                                    >  2:792393/1‑140 (MQ=255)                        aaaaaaGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGaaa                                                                                                                <  2:81392/140‑1 (MQ=255)                             aGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAg                                                                                                           <  2:656929/140‑1 (MQ=255)                                    gAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGt                                                                                                    <  2:375372/140‑1 (MQ=255)                                            aaaTAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTg                                                                                            <  2:787743/140‑1 (MQ=255)                                            aaaTAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTg                                                                                            <  2:1121113/140‑1 (MQ=255)                                              ataatCTCAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAGTGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCg                                                                                          <  2:463798/140‑1 (MQ=255)                                                  tCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGt                                                                                      >  1:918517/1‑140 (MQ=255)                                                   tttAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGtt                                                                                     <  2:340778/139‑1 (MQ=255)                                                   cTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGtt                                                                                     <  2:938999/140‑1 (MQ=255)                                                   cTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGACATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGtt                                                                                     <  1:972356/140‑1 (MQ=255)                                                     tAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTc                                                                                                   <  1:129272/124‑1 (MQ=255)                                                     tAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTc                                                                                                   >  2:129272/1‑124 (MQ=255)                                                       aTTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGaa                                                                                 >  1:1253212/1‑140 (MQ=255)                                                            ccTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGt                                                                            >  2:787213/1‑140 (MQ=255)                                                                     tgtgCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCaa                                                                   >  1:984922/1‑140 (MQ=255)                                                                       tgCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACa                                                                 >  2:662501/1‑140 (MQ=255)                                                                        gCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACAt                                                                <  1:662501/140‑1 (MQ=255)                                                                            aCCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGc                                                            >  1:82721/1‑140 (MQ=255)                                                                                 ccAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATaaa                                                       >  2:716959/1‑140 (MQ=255)                                                                                 ccAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATaaa                                                       >  1:1063994/1‑140 (MQ=255)                                                                                   aGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATg                                                     <  1:123474/140‑1 (MQ=255)                                                                                   aGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATg                                                     <  1:792393/140‑1 (MQ=255)                                                                                   aGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATg                                                     <  2:47876/140‑1 (MQ=255)                                                                                   aGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATg                                                     <  1:716959/140‑1 (MQ=255)                                                                                      aaCAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCg                                                  <  1:1126644/140‑1 (MQ=255)                                                                                        cAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCt                                                >  1:573078/1‑140 (MQ=255)                                                                                           gtatgtcAAAGGCACTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAAt                                             >  2:963642/7‑140 (MQ=255)                                                                                           ggATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAAt                                             >  1:418425/1‑140 (MQ=255)                                                                                           ggATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAAt                                             >  1:99140/1‑140 (MQ=255)                                                                                           ggATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAAt                                             >  2:37369/1‑140 (MQ=255)                                                                                           ggATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAAt                                             >  2:359447/1‑140 (MQ=255)                                                                                             aTCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGa                                           <  2:573078/140‑1 (MQ=255)                                                                                             aTCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGa                                           <  2:741349/140‑1 (MQ=255)                                                                                               cGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCt                                                <  2:1137616/133‑1 (MQ=255)                                                                                               cGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCt                                                >  1:1137616/1‑133 (MQ=255)                                                                                                  aaTGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATg                                      <  1:108533/140‑1 (MQ=255)                                                                                                   aTGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGc                                     >  2:302181/1‑140 (MQ=255)                                                                                                     ggCATTTGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCaa                                   >  1:807230/1‑140 (MQ=255)                                                                                                         tttGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGt                               >  1:1229328/1‑140 (MQ=255)                                                                                                         tttGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGt                               >  1:619009/1‑140 (MQ=255)                                                                                                          ttGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTg                              >  1:343250/1‑140 (MQ=255)                                                                                                           tGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGa                             <  1:359447/140‑1 (MQ=255)                                                                                                           tGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGa                             <  1:205834/140‑1 (MQ=255)                                                                                                           tGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGa                             <  1:456483/140‑1 (MQ=255)                                                                                                           tGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGa                             >  1:650630/1‑140 (MQ=255)                                                                                                           tGCTACACCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGa                             <  2:984922/140‑1 (MQ=255)                                                                                                                cacCAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCAc                        >  1:1064565/1‑140 (MQ=255)                                                                                                                  ccAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCa                      <  1:1060866/140‑1 (MQ=255)                                                                                                                  ccAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCa                      <  1:963642/140‑1 (MQ=255)                                                                                                                   cAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCAt                     >  2:683706/1‑140 (MQ=255)                                                                                                                   cAAACGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCAt                     >  2:868948/1‑140 (MQ=255)                                                                                                                     aaCGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATaa                   >  2:712649/1‑140 (MQ=255)                                                                                                                     aaCGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATaa                   >  2:465512/1‑140 (MQ=255)                                                                                                                     aaCGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATaa                   >  2:143098/1‑140 (MQ=255)                                                                                                                      aCGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAAc                  <  2:807230/140‑1 (MQ=255)                                                                                                                       cGAGGTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAACg                 >  1:276399/1‑140 (MQ=255)                                                                                                                          ggTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAACGTcc              >  1:892907/1‑140 (MQ=255)                                                                                                                          ggTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAACGTcc              <  2:431797/140‑1 (MQ=255)                                                                                                                          ggTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAACGTcc              <  2:337140/140‑1 (MQ=255)                                                                                                                           gTGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAACGTccc             >  1:917437/1‑140 (MQ=255)                                                                                                                            tGATAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAACGTCCCa            <  2:619009/140‑1 (MQ=255)                                                                                                                              aTAACCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAACGTCCCAcc          >  2:716443/1‑140 (MQ=255)                                                                                                                                aaCCCAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAACGTCCCACCgg        >  2:1099007/1‑140 (MQ=255)                                                                                                                                    cAGGTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATGACGTCCCACCGGTTaa    >  2:354709/1‑140 (MQ=255)                                                                                                                                      ggTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAACGTCCCACCGGTTAATg  >  1:572607/1‑140 (MQ=255)                                                                                                                                      ggTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAACGTCCCACCGGTTAATg  >  2:731133/1‑140 (MQ=255)                                                                                                                                      ggTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAACGTCCCACCGGTTAATg  >  2:135015/1‑140 (MQ=255)                                                                                                                                      ggTGTCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAACGTCCCACCGGTTAATg  >  1:97554/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACATTTGCATAAATGTCGCTAATGACAATGCAATAGTGACGCACCATAACGTCCCACCGGTTAATG  >  NC_000913/2481876‑2482148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑