Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,601,501	G→A	G101R (GGA→AGA)	hyfA →	hydrogenase 4 component A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,601,501	0	G	A	100.0%	267.6 / NA	75	G101R (GGA→AGA)	hyfA	hydrogenase 4 component A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/40);  total (35/40)

GGAAGCCCCTTGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGT  >  NC_000913/2601365‑2601631                                                                                                                                         |                                                                                                                                   ggAAGCCCCTTGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGaa                                                                                                                                 <  2:492758/140‑1 (MQ=255)  gAAGCCCCTTGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCaagaag                                                                                                                                <  2:224633/140‑1 (MQ=255)  gAAGCCCCTTGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCaagaag                                                                                                                                <  2:207966/140‑1 (MQ=255)     gCCCCTTGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCg                                                                                                                             >  2:484642/1‑140 (MQ=255)      ccccTTGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGt                                                                                                                            >  2:408312/1‑140 (MQ=255)      ccccTTGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGt                                                                                                                            >  1:178825/1‑140 (MQ=255)         cTTGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCg                                                                                                                         <  2:16312/140‑1 (MQ=255)           tGCCTGCAGGTCTGCCCGGTTAATGCCATCTCTCAGAGCGATCATGCGATCCAACTCATCGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGCTCTGTCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGt                                                                                                                       <  2:1090332/140‑1 (MQ=255)              cTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGaa                                                                                                                    <  1:412448/140‑1 (MQ=255)                 cAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGc                                                                                                                 >  2:498981/1‑140 (MQ=255)                   ggTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGccc                                                                                                               <  2:1252238/140‑1 (MQ=255)                      cTGCCCGGTAAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATg                                                                                                            <  2:219994/140‑1 (MQ=255)                       tGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATgc                                                                                                           <  2:749903/140‑1 (MQ=255)                       tGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATg                                                                                                            >  2:905143/1‑139 (MQ=255)                       tGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATg                                                                                                            <  1:905143/139‑1 (MQ=255)                       tGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTCGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATgc                                                                                                           >  1:514769/1‑140 (MQ=255)                          ccGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCa                                                                                                        <  1:498137/140‑1 (MQ=255)                          ccGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCa                                                                                                        <  2:191829/140‑1 (MQ=255)                          ccGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCa                                                                                                        <  1:395948/140‑1 (MQ=255)                          ccGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTCGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCa                                                                                                        <  2:514769/140‑1 (MQ=255)                              tCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATgc                                                                                                           >  1:485699/1‑133 (MQ=255)                              tCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATgc                                                                                                           <  2:485699/133‑1 (MQ=255)                                   gCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGtttt                                                                                               <  1:318267/140‑1 (MQ=255)                                     cATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTc                                                                                             <  2:824925/140‑1 (MQ=255)                                        ctctcAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAgg                                                                                          >  2:1021180/1‑140 (MQ=255)                                            cAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGa                                                                                      >  1:950354/1‑140 (MQ=255)                                                 ggATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGTGCAATATGTTTTTCAGGCTGAAGGct                                                                                 <  2:1194058/140‑1 (MQ=255)                                                 ggATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGct                                                                                 >  2:892101/1‑140 (MQ=255)                                                 ggATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGct                                                                                 <  1:1021180/140‑1 (MQ=255)                                                 ggATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTACGCCGTGGTCTACCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGCGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGct                                                                                 >  1:1087894/1‑140 (MQ=255)                                                   atgatgCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATTCCCATGCGCAATATGTTTTTCAGGCTGAAGGctca                                                                               >  2:889403/1‑140 (MQ=255)                                                   atgatgCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGCCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGctca                                                                               >  1:841613/1‑140 (MQ=255)                                                    tgatgCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGctcac                                                                              >  1:505635/1‑140 (MQ=255)                                                    tgatgCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGctcac                                                                              >  2:762774/1‑140 (MQ=255)                                                     gatgCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGctcact                                                                             >  1:393835/1‑140 (MQ=255)                                                       tgCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGctcactca                                                                           >  1:953449/1‑140 (MQ=255)                                                       tgCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCACATGCGCAATATGTTTTTCAGGCTGAAGGct                                                                                 <  2:1004357/134‑1 (MQ=255)                                                       tgCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCACATGCGCAATATGTTTTTCAGGCTGAAGGct                                                                                 >  1:1004357/1‑134 (MQ=255)                                                         cGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCaaa                                                                         >  1:102969/1‑140 (MQ=255)                                                          gATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAg                                                                        <  1:1231895/140‑1 (MQ=255)                                                              cAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACgg                                                                    <  1:342345/140‑1 (MQ=255)                                                                 cTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTCGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCga                                                                 >  2:106941/1‑140 (MQ=255)                                                                  tCAACGAAAGCCTCTGTATTGCCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGGCGTCCGGTGAATGCCCATGCGAAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCgaa                                                                <  2:161637/140‑1 (MQ=255)                                                                      cGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGaaa                                                            >  2:424341/1‑140 (MQ=255)                                                                              tctGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCca                                                    <  2:8257/140‑1 (MQ=255)                                                                              tctGTATTGGCTGCAAGCTTTGCGCCGTGGCCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCca                                                    <  2:841613/140‑1 (MQ=255)                                                                                tGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCca                                                    >  1:1008787/1‑138 (MQ=255)                                                                                tGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCca                                                    <  2:1008787/138‑1 (MQ=255)                                                                                      ggCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCcaa                                                   >  2:1265863/1‑133 (MQ=255)                                                                                      ggCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCcaa                                                   <  1:1265863/133‑1 (MQ=255)                                                                                      ggCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCcaa                                                   <  2:411223/133‑1 (MQ=255)                                                                                      ggCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCcaa                                                   >  1:411223/1‑133 (MQ=255)                                                                                       gCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACAt                                           >  1:394759/1‑140 (MQ=255)                                                                                       gCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACAt                                           >  2:434642/1‑140 (MQ=255)                                                                                        cTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATg                                          <  1:424341/140‑1 (MQ=255)                                                                                        cTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATg                                          >  2:651684/1‑140 (MQ=255)                                                                                         tGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGc                                         >  2:736886/1‑140 (MQ=255)                                                                                            aaGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCttt                                      >  2:1211973/1‑140 (MQ=255)                                                                                            aaGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCttt                                      >  2:91259/1‑140 (MQ=255)                                                                                            aaGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCttt                                      >  1:340836/1‑140 (MQ=255)                                                                                            aaGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCttt                                      >  2:629906/1‑140 (MQ=255)                                                                                                tttGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTtgctg                                  <  2:953449/140‑1 (MQ=255)                                                                                                   gcgcCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTgcgc                               <  2:393835/140‑1 (MQ=255)                                                                                                    cgcCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCt                              <  1:663408/140‑1 (MQ=255)                                                                                                    cgcCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCt                              <  2:775088/140‑1 (MQ=255)                                                                                                        gTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGa                          <  2:45605/140‑1 (MQ=255)                                                                                                        gTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGa                          <  1:1211973/140‑1 (MQ=255)                                                                                                          ggTCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAAc                        <  2:1174062/140‑1 (MQ=255)                                                                                                            tCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCt                      <  1:914484/140‑1 (MQ=255)                                                                                                            tCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCt                      <  1:1028574/140‑1 (MQ=255)                                                                                                            tCTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCt                      <  2:178825/140‑1 (MQ=255)                                                                                                             cTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTg                     <  1:498981/140‑1 (MQ=255)                                                                                                             cTGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTg                     <  2:340836/140‑1 (MQ=255)                                                                                                              tGCCCATTTGGCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTgg                    >  2:1161139/1‑140 (MQ=255)                                                                                                                       ggCGCAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGAc           <  2:505635/140‑1 (MQ=255)                                                                                                                          gcAATCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGt        >  2:831949/1‑140 (MQ=255)                                                                                                                             aTCAGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTcgc     >  1:519157/1‑140 (MQ=255)                                                                                                                                aGCGCTTCAAGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGt  <  1:408312/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   GGAAGCCCCTTGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGT  >  NC_000913/2601365‑2601631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑