Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,658,666	T→C	G90G (GGA→GGG)	hscA ←	iron‑sulfur cluster biosynthesis chaperone HscA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,658,666	0	T	C	100.0%	256.8 / NA	68	G90G (GGA→GGG)	hscA	iron‑sulfur cluster biosynthesis chaperone HscA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/38);  total (30/38)

GAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGTCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACGGCCTTCATGAT  >  NC_000913/2658534‑2658799                                                                                                                                     |                                                                                                                                      gAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCa                                                                                                                                >  1:1111559/1‑140 (MQ=255)  aGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAg                                                                                                                               <  2:1111559/140‑1 (MQ=255)    gATGTCCGCAGAAACGCGCACCGGGTTCAGTAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGc                                                                                                                             >  1:907684/1‑140 (MQ=255)       gTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATc                                                                                                                                 >  2:195303/1‑133 (MQ=255)       gTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATc                                                                                                                                 <  1:195303/133‑1 (MQ=255)       gTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGtt                                                                                                                          >  2:933435/1‑140 (MQ=255)       gTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGtt                                                                                                                          <  2:350671/140‑1 (MQ=255)       gTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGtt                                                                                                                          <  1:255710/140‑1 (MQ=255)          cgtaGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTaa                                                                                                                       >  2:749241/4‑140 (MQ=255)          cGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTaa                                                                                                                       >  1:845283/1‑140 (MQ=255)           gCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAAc                                                                                                                      <  1:851181/140‑1 (MQ=255)           gCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAAc                                                                                                                      <  2:650363/140‑1 (MQ=255)           gCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAAc                                                                                                                      <  2:1080519/140‑1 (MQ=255)           gCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAAc                                                                                                                      <  1:354790/140‑1 (MQ=255)           gCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAAc                                                                                                                      <  1:1027903/140‑1 (MQ=255)            cAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACa                                                                                                                     >  2:1027161/1‑140 (MQ=255)                    cgcACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAAt                                                                                                             >  1:1206343/1‑140 (MQ=255)                              tCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCg                                                                                                   <  2:111571/140‑1 (MQ=255)                                agcagcCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGt                                                                                                 >  2:151877/1‑140 (MQ=255)                                      cccGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAg                                                                                           >  2:392691/1‑140 (MQ=255)                                      cccGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAg                                                                                           <  1:933435/140‑1 (MQ=255)                                      cccGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAg                                                                                           <  1:1080663/140‑1 (MQ=255)                                        cgccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAgcg                                                                                         <  1:132282/140‑1 (MQ=255)                                        cgccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAgcg                                                                                         <  2:774828/140‑1 (MQ=255)                                        cgccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAgcg                                                                                         <  1:1204357/140‑1 (MQ=255)                                        cgccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAgcg                                                                                         <  2:1209949/140‑1 (MQ=255)                                        cgccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAgcg                                                                                         <  2:1257378/140‑1 (MQ=255)                                        cgccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAgcg                                                                                         <  1:510877/140‑1 (MQ=255)                                        cgccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAgcg                                                                                         <  1:151877/140‑1 (MQ=255)                                        cgccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAgcg                                                                                         <  1:292707/140‑1 (MQ=255)                                          ccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCg                                                                                                   <  1:27549/128‑1 (MQ=255)                                          ccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCg                                                                                                   >  2:27549/1‑128 (MQ=255)                                          ccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCt                                                                                       >  1:892661/1‑140 (MQ=255)                                          ccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCt                                                                                       >  2:459593/1‑140 (MQ=255)                                          ccgccgTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCt                                                                                       >  1:255530/1‑140 (MQ=255)                                                     atcatcGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGTGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTAcg                                                                            <  1:1119887/140‑1 (MQ=255)                                                         tcGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTAcgcgcg                                                                        <  1:153199/140‑1 (MQ=255)                                                         tcGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTAcgcgcg                                                                        <  1:564842/140‑1 (MQ=255)                                                          cGGCAGACCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTAcg                                                                            >  1:770535/1‑135 (MQ=255)                                                          cGGCAGACCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTAcg                                                                            <  2:770535/135‑1 (MQ=255)                                                              aGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATa                                                                   <  1:668494/140‑1 (MQ=255)                                                                gCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAAc                                                                 >  1:711316/1‑140 (MQ=255)                                                                gCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACACGCGTCATAAc                                                                 >  2:569171/1‑140 (MQ=255)                                                                        cGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGaa                                                         <  2:1206343/140‑1 (MQ=255)                                                                             gCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGccc                                                    <  1:439801/140‑1 (MQ=255)                                                                              ccTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCt                                                   >  2:78182/1‑140 (MQ=255)                                                                                tGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTg                                                 <  1:78182/140‑1 (MQ=255)                                                                                         tAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATa                                        <  2:762790/140‑1 (MQ=255)                                                                                            ggCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTg                                     >  2:248172/1‑140 (MQ=255)                                                                                            ggCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTg                                     >  1:817408/1‑140 (MQ=255)                                                                                             gCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGa                                    >  2:1219776/1‑140 (MQ=255)                                                                                              cAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGaa                                   <  2:845283/140‑1 (MQ=255)                                                                                                 aTGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGaacaa                                <  2:907684/140‑1 (MQ=255)                                                                                                 aTGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGaacaa                                <  1:330071/140‑1 (MQ=255)                                                                                                 aTGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGaacaa                                >  1:225102/1‑140 (MQ=255)                                                                                                       aTAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATg                          >  2:1113040/1‑140 (MQ=255)                                                                                                        tAGCGTTGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATgg                         >  1:1088948/1‑140 (MQ=255)                                                                                                             ttGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGgcagca                    <  1:248172/140‑1 (MQ=255)                                                                                                              tGCTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGcagcag                   >  1:76377/1‑140 (MQ=255)                                                                                                                cTGGATATCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGt                 >  1:677770/1‑140 (MQ=255)                                                                                                                    atatCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACg             >  2:591695/1‑140 (MQ=255)                                                                                                                    atatCAGCCAGCGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACg             <  1:1027161/140‑1 (MQ=255)                                                                                                                        cagccagcGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACGGCCt         >  1:1068323/1‑140 (MQ=255)                                                                                                                         agccagcGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACGGCCtt        <  2:677770/140‑1 (MQ=255)                                                                                                                           ccagcGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACGGCCt         <  1:1114780/137‑1 (MQ=255)                                                                                                                           ccagcGAGCGCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACGGCCt         >  2:1114780/1‑137 (MQ=255)                                                                                                                             agcgagcgCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAgg                  >  2:757538/1‑126 (MQ=255)                                                                                                                             agcgagcgCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAgg                  <  1:757538/126‑1 (MQ=255)                                                                                                                               cgagcgCCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACGGCCTTCATGAt  >  2:190433/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      GAGGATGTCCGCAGAAACGCGCACCGGGTTCAGCAGCCCCGCCGCCGTTTCAATCATCGGCAGGCCGTTTTCGCTGGCCTGGAATTGATAAGGCAGATGCGGATAGCGTTGCTGGATATCAGCCAGCGAGCGTCCCATCAGGCGTTTAACAGAACTAATTGTGTTGGCGGTATCGAGCGCTGCATTAGTACGCGCGTCATAACCCACCGAATGCCCTTGCTGTTGATAGTGAACAACAGATGGCAGCAGGTGACGGCCTTCATGAT  >  NC_000913/2658534‑2658799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑