Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,716,589	T→C	intergenic (‑140/‑165)	grcA ← / → ung	stress‑induced alternate pyruvate formate‑lyase subunit/uracil‑DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,716,589	0	T	C	100.0%	252.3 / NA	67	intergenic (‑140/‑165)	grcA/ung	stress‑induced alternate pyruvate formate‑lyase subunit/uracil‑DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/25);  total (42/25)

CCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGATTTTGTTCTGTAGAAAGAAGCAGTTAAGCTA  >  NC_000913/2716460‑2716724                                                                                                                                  |                                                                                                                                        cccGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCaa                                                                                                                               >  2:1035984/1‑140 (MQ=255) cccGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCaa                                                                                                                               >  2:1034020/1‑140 (MQ=255) cccGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCaa                                                                                                                               >  1:574708/1‑140 (MQ=255)      cAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGa                                                                                                                          <  2:603833/140‑1 (MQ=255)           ttattTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTa                                                                                                                     <  2:1247546/140‑1 (MQ=255)                 gatttgTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGttt                                                                                                               <  1:1035984/140‑1 (MQ=255)                 gatttgTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGttt                                                                                                               <  1:967456/140‑1 (MQ=255)                            ccTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATTTATACTCCTCAAACACCCGTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTGTTGTTTTACATCAACtt                                                                                                    >  2:1141405/1‑140 (MQ=255)                               gTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATg                                                                                                 <  2:574708/140‑1 (MQ=255)                                tCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGc                                                                                                >  2:765216/1‑140 (MQ=255)                                tCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGc                                                                                                >  1:588570/1‑140 (MQ=255)                                tCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGc                                                                                                >  2:91630/1‑140 (MQ=255)                                                       tgttgCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTa                                                                         >  1:326879/1‑140 (MQ=255)                                                            cTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACAttt                                                                    >  2:692143/1‑140 (MQ=255)                                                            cTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACAttt                                                                    >  1:912970/1‑140 (MQ=255)                                                                  tgtATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGc                                                              <  1:765216/140‑1 (MQ=255)                                                                  tgtATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGc                                                              <  1:91630/140‑1 (MQ=255)                                                                    tatatataCTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCAc                                                            <  1:857479/140‑1 (MQ=255)                                                                      tatataCTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTc                                                          >  1:631145/1‑140 (MQ=255)                                                                           aCTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGTGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTa                                                     >  1:268274/1‑140 (MQ=255)                                                                            ctcctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTaa                                                    >  2:1118366/1‑140 (MQ=255)                                                                            ctcctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTaa                                                    >  2:947448/1‑140 (MQ=255)                                                                            ctcctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTaa                                                    >  2:1238194/1‑140 (MQ=255)                                                                            ctcctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTaa                                                    >  1:242542/1‑140 (MQ=255)                                                                            ctcctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTaa                                                    >  2:901747/1‑140 (MQ=255)                                                                              cctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTaa                                                    <  1:485440/138‑1 (MQ=255)                                                                              cctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTaa                                                    >  2:485440/1‑138 (MQ=255)                                                                              cctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAg                                                   <  1:1201484/139‑1 (MQ=255)                                                                              cctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAg                                                   >  2:1201484/1‑139 (MQ=255)                                                                              cctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGt                                                  >  1:916953/1‑140 (MQ=255)                                                                              cctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGt                                                  >  1:638122/1‑140 (MQ=255)                                                                              cctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGt                                                  >  1:506478/1‑140 (MQ=255)                                                                              cctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGt                                                  >  2:1053694/1‑140 (MQ=255)                                                                              cctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGt                                                  >  1:387877/1‑140 (MQ=255)                                                                              cctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGt                                                  >  1:1122592/1‑140 (MQ=255)                                                                              cctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGt                                                  >  2:1196673/1‑140 (MQ=255)                                                                              cctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTAGCAATCGTTTAGGt                                                  >  2:787372/1‑140 (MQ=255)                                                                               ctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTc                                                 >  2:969115/1‑140 (MQ=255)                                                                               ctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTc                                                 >  2:884849/1‑140 (MQ=255)                                                                               ctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTc                                                 >  1:260041/1‑140 (MQ=255)                                                                               ctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTc                                                 >  1:798523/1‑140 (MQ=255)                                                                               ctcAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACA‑TTTTTTGCACTCGTTTAAGTCt                                                >  1:1216045/1‑140 (MQ=255)                                                                                  aaaCACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTaa                                              >  2:30501/1‑140 (MQ=255)                                                                                    acacCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTaaaa                                            >  2:419177/1‑140 (MQ=255)                                                                                       cccTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAAt                                         <  2:387877/140‑1 (MQ=255)                                                                                       cccTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAAt                                         >  2:681047/1‑140 (MQ=255)                                                                                         cTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTaa                                                    >  2:1218692/1‑127 (MQ=255)                                                                                         cTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTaa                                                    <  1:1218692/127‑1 (MQ=255)                                                                                         cTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGa                                       >  2:597159/1‑140 (MQ=255)                                                                                         cTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGACGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGa                                       >  2:1041565/1‑140 (MQ=255)                                                                                            gAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCa                                    <  1:33617/140‑1 (MQ=255)                                                                                            gAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCa                                    <  1:681047/140‑1 (MQ=255)                                                                                            gAATCTTTGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCa                                    <  2:631145/140‑1 (MQ=255)                                                                                                 tttGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGAtt                               <  1:1118366/140‑1 (MQ=255)                                                                                                  ttGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGAttt                              >  1:964819/1‑140 (MQ=255)                                                                                                   tGATTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGTGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGAtttt                             <  2:268274/140‑1 (MQ=255)                                                                                                     aTTTAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGATTTTGt                           <  2:916953/140‑1 (MQ=255)                                                                                                      tttAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGATTTTGtt                          <  2:588570/140‑1 (MQ=255)                                                                                                      tttAAATCAATAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGATTTTGtt                          <  1:787372/140‑1 (MQ=255)                                                                                                              aaTAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGATTTTGTTCTGTagaa                  <  2:798523/140‑1 (MQ=255)                                                                                                                tAAAAACCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACA‑TTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGATTTTGTTCTGTagaaaga               <  2:1216045/140‑1 (MQ=255)                                                                                                                tAAAAACCACACATCAAGCATGGTCACAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGATTTTGTTCTGTagaaag                >  1:946941/1‑140 (MQ=255)                                                                                                                 aaaaaCCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGATTTTGTTCTGTagaaaga               <  1:947448/140‑1 (MQ=255)                                                                                                                  aaaaCCACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGATTTTGTTCTGTagaaagaa              <  2:964819/140‑1 (MQ=255)                                                                                                                      ccACACATCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGATTTTGTTCTGTAGAAAGAAGCAg          <  2:242542/140‑1 (MQ=255)                                                                                                                          acaTCAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGATTTTGTTCTGTAGAAAGAAGCAGTTaa      >  1:936712/1‑140 (MQ=255)                                                                                                                              cAAGCATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGATTTTGTTCTGTAGAAAGAAGCAGTTAAGCTa  >  1:696661/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        CCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCGGGTGTGAAATTTTACCAATTTACATTTTTTTGCACTCGTTTAAGTCTAAAAAATGAGCATGATTTTGTTCTGTAGAAAGAAGCAGTTAAGCTA  >  NC_000913/2716460‑2716724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑