Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	262,808	C→T	intergenic (+52/‑63)	proA → / → thrW	glutamate‑5‑semialdehyde dehydrogenase/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	262,808	0	C	T	100.0%	327.1 / NA	91	intergenic (+52/‑63)	proA/thrW	glutamate‑5‑semialdehyde dehydrogenase/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (48/43);  total (48/43)

CTCCACGCGCGTGGCCCAATGGGGCTGGAAGCACTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGCATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCA  >  NC_000913/262673‑262943                                                                                                                                          |                                                                                                                                        cTCCACGCGCGTGGCCCAATGGGGCTGGAAGCACTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCg                                                                                                                                     >  2:1176010/1‑140 (MQ=255)   ccACGCGCGTGGCCCAATGGGGCTGGAAGCACTGACCACTTACAAGTGGATCGGCATTAGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGcc                                                                                                                                   <  1:1257838/140‑1 (MQ=255)       gcgcgTGGCCCAATGGGGCTGGAAGCACTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGt                                                                                                                               <  1:45643/140‑1 (MQ=255)            tGGCCCAATGGGGCTGGAAGCACTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGtt                                                                                                                          >  1:863294/1‑140 (MQ=255)            tGGCCCAATGGGGCTGGAAGCACTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGtt                                                                                                                          >  1:962131/1‑140 (MQ=255)              gCCCAATGGGGCTGGAAGCACTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGtttt                                                                                                                        >  2:601001/1‑140 (MQ=255)                  aaTGGGGCTGGAAGCACTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAAcc                                                                                                                    <  1:551256/140‑1 (MQ=255)                         cTGGAAGCACTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCa                                                                                                             >  2:1126406/1‑140 (MQ=255)                         cTGGAAGCACTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCa                                                                                                             >  1:366977/1‑140 (MQ=255)                         cTGGAAGCACTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCa                                                                                                             >  2:1115871/1‑140 (MQ=255)                                  cTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCg                                                                                                          <  1:745943/134‑1 (MQ=255)                                  cTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCg                                                                                                          >  2:745943/1‑134 (MQ=255)                                  cTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTc                                                                                                    >  2:230953/1‑140 (MQ=255)                                  cTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTc                                                                                                    >  1:147954/1‑140 (MQ=255)                                   tGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCa                                                                                                   <  1:368454/140‑1 (MQ=255)                                     aCCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGt                                                                                                                <  2:748962/125‑1 (MQ=255)                                     aCCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGt                                                                                                                >  1:748962/1‑125 (MQ=255)                                     aCCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACg                                                                                                 <  1:1106818/140‑1 (MQ=255)                                      ccACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGt                                                                                                >  1:209051/1‑140 (MQ=255)                                      ccACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGt                                                                                                >  1:652686/1‑140 (MQ=255)                                      ccACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGt                                                                                                >  1:77259/1‑140 (MQ=255)                                           tACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTg                                                                                           <  2:45232/140‑1 (MQ=255)                                             cAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGaa                                                                                         <  2:1082972/140‑1 (MQ=255)                                             cAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGaa                                                                                         <  2:162620/140‑1 (MQ=255)                                                 tGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATg                                                                                     >  1:826463/1‑140 (MQ=255)                                                  ggATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGt                                                                                    <  1:601001/140‑1 (MQ=255)                                                    aTCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTcc                                                                                  >  1:649586/1‑140 (MQ=255)                                                    aTCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTcc                                                                                  >  1:121987/1‑140 (MQ=255)                                                        gCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTc                                                                              >  2:369412/1‑140 (MQ=255)                                                             ggTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGc                                                                         <  2:366977/140‑1 (MQ=255)                                                             ggTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGc                                                                         <  1:1126406/140‑1 (MQ=255)                                                             ggTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGc                                                                         <  2:863294/140‑1 (MQ=255)                                                             ggTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGc                                                                         <  1:230642/140‑1 (MQ=255)                                                                 aTTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGat                                                                     <  2:652686/140‑1 (MQ=255)                                                                   tACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAgg                                                                           >  2:415882/1‑132 (MQ=255)                                                                   tACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAgg                                                                           <  1:415882/132‑1 (MQ=255)                                                                    acacCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATTCACACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGc                                                                         <  1:906431/135‑1 (MQ=255)                                                                    acacCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATTCACACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGc                                                                         >  2:906431/1‑135 (MQ=255)                                                                    acacCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGAtata                                                                  <  2:209051/140‑1 (MQ=255)                                                                    acacCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGAtata                                                                  <  1:564236/140‑1 (MQ=255)                                                                    acacCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGAtata                                                                  <  2:77259/140‑1 (MQ=255)                                                                      acCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGc                                                                <  1:442047/140‑1 (MQ=255)                                                                      acCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGc                                                                >  1:259589/1‑140 (MQ=255)                                                                        cATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTc                                                              >  1:679958/1‑140 (MQ=255)                                                                        cATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTc                                                              >  1:603537/1‑140 (MQ=255)                                                                        cATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTc                                                              >  1:87936/1‑140 (MQ=255)                                                                         aTTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCa                                                             <  1:1197525/140‑1 (MQ=255)                                                                          ttCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGa                                                                      >  1:590811/1‑130 (MQ=255)                                                                          ttCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGa                                                                      <  2:590811/130‑1 (MQ=255)                                                                          ttCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGc                                                                >  1:751498/1‑136 (MQ=255)                                                                          ttCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGc                                                                <  2:751498/136‑1 (MQ=255)                                                                          ttCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCa                                                             >  2:299671/1‑139 (MQ=255)                                                                          ttCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCa                                                             <  1:299671/139‑1 (MQ=255)                                                                          ttCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAg                                                            >  2:1242847/1‑140 (MQ=255)                                                                          ttCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAg                                                            >  1:1044292/1‑140 (MQ=255)                                                                           tCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCa                                                             <  2:668819/138‑1 (MQ=255)                                                                           tCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCa                                                             >  1:668819/1‑138 (MQ=255)                                                                           tCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGt                                                           >  2:615260/1‑140 (MQ=255)                                                                               gCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGt                                                       >  2:891018/1‑140 (MQ=255)                                                                                cGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCa                                                             >  1:31042/1‑133 (MQ=255)                                                                                cGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCa                                                             <  2:31042/133‑1 (MQ=255)                                                                                cGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTa                                                      >  1:483856/1‑140 (MQ=255)                                                                                  taaataaaACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGc                                                                >  1:997896/1‑128 (MQ=255)                                                                                  taaataaaACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGc                                                                <  2:997896/128‑1 (MQ=255)                                                                                      taaaACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGagcag                                                <  1:1242847/140‑1 (MQ=255)                                                                                              ggTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCg                                        >  1:218834/1‑140 (MQ=255)                                                                                              ggTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCg                                        >  1:920697/1‑140 (MQ=255)                                                                                              ggTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCg                                        >  1:569913/1‑140 (MQ=255)                                                                                                   tGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATg                                   >  1:69385/1‑140 (MQ=255)                                                                                                     cAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCg                                 <  2:98949/140‑1 (MQ=255)                                                                                                     cAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCg                                 <  2:679958/140‑1 (MQ=255)                                                                                                     cAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCg                                 <  1:302157/140‑1 (MQ=255)                                                                                                     cAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCg                                 <  2:603537/140‑1 (MQ=255)                                                                                                     cAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGATCAGTTGGTAGAGCAGCGCATTCGTAATGCg                                 <  1:230953/140‑1 (MQ=255)                                                                                                      aaaaGTAGCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGa                                <  2:121987/140‑1 (MQ=255)                                                                                                             gCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCGGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGt                         >  2:950520/1‑140 (MQ=255)                                                                                                             gCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGt                         >  2:837751/1‑140 (MQ=255)                                                                                                             gCCATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGt                         <  2:218834/140‑1 (MQ=255)                                                                                                              ccATTTGATT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTa                        >  1:998508/1‑140 (MQ=255)                                                                                                                     aTT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                 >  1:260754/1‑140 (MQ=255)                                                                                                                     aTT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                 <  1:587973/140‑1 (MQ=255)                                                                                                                     aTT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                 <  2:87936/140‑1 (MQ=255)                                                                                                                     aTT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                 <  2:147954/140‑1 (MQ=255)                                                                                                                     aTT‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                 <  1:369412/140‑1 (MQ=255)                                                                                                                      tt‑‑CACAAGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGAc                <  1:615260/140‑1 (MQ=255)                                                                                                                             aaGGCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCta           >  1:102209/1‑140 (MQ=255)                                                                                                                               ggCCATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCtatt         >  1:792835/1‑140 (MQ=255)                                                                                                                                 ccATTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCtattat       <  2:962131/140‑1 (MQ=255)                                                                                                                                  cATTGACGTATCGCCCGGTTAGTTTTAGCCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATc      >  1:169698/1‑140 (MQ=255)                                                                                                                                   aTTGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCg     >  2:928948/1‑140 (MQ=255)                                                                                                                                     tGACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGc   <  2:569913/140‑1 (MQ=255)                                                                                                                                      gACGTATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCa  >  2:29043/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CTCCACGCGCGTGGCCCAATGGGGCTGGAAGCACTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAAATAAAACCGGGTGATGCAAAAGTAGCCATTTGATT‑‑CACAAGGCCATTGACGCATCGCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCA  >  NC_000913/262673‑262943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑