Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,824,303	T→C	Q15R (CAG→CGG)	pncC ←	NMN aminohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,824,303	0	T	C	100.0%	215.0 / NA	57	Q15R (CAG→CGG)	pncC	NMN aminohydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/31);  total (26/31)

TGGCTTCGTTACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACG  >  NC_000913/2824166‑2824428                                                                                                                                          |                                                                                                                              tGGCTTCGTTACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGc                                                                                                                             >  1:1261837/1‑140 (MQ=255)   gCTTCGTTACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCgcccgccc                                                                                                                           <  1:748906/140‑1 (MQ=255)    cTTCGTTACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCa                                                                                                                          <  1:395558/140‑1 (MQ=255)     ttCGTTACTGTAGGTGACAAATCCGCGTTC‑AACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAAc                                                                                                                        <  1:764640/140‑1 (MQ=255)      tCGTTACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAAc                                                                                                                        <  1:529485/140‑1 (MQ=255)        gTTACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCt                                                                                                                      <  1:848342/140‑1 (MQ=255)         ttACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCt                                                                                                                      >  1:80180/1‑139 (MQ=255)         ttACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCt                                                                                                                      <  2:80180/139‑1 (MQ=255)                 ggTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTa                                                                                                             <  1:992355/140‑1 (MQ=255)                 ggTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTa                                                                                                             >  2:77962/1‑140 (MQ=255)                 ggTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTa                                                                                                             >  1:1094076/1‑140 (MQ=255)                  gTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTaa                                                                                                            <  2:472179/140‑1 (MQ=255)                   tGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAAc                                                                                                           <  1:53105/140‑1 (MQ=255)                   tGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAAc                                                                                                           <  2:1094076/140‑1 (MQ=255)                    gACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACt                                                                                                          >  1:267981/1‑140 (MQ=255)                     aCAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTg                                                                                                         <  2:954621/140‑1 (MQ=255)                      cAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTCAACTGc                                                                                                        >  1:966255/1‑140 (MQ=255)                       aaaTCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGtt                                                                                                                   >  1:151055/1‑128 (MQ=255)                       aaaTCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGtt                                                                                                                   <  2:151055/128‑1 (MQ=255)                        aaTCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCAt                                                                                                      <  2:267981/140‑1 (MQ=255)                                ttCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCAc                                                                                              <  2:594492/140‑1 (MQ=255)                                   aaaCCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTCAACTGCATCAGTTCACTgt                                                                                           <  2:966255/140‑1 (MQ=255)                                     aCCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgtca                                                                                         <  1:17696/140‑1 (MQ=255)                                      ccAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgtcag                                                                                        <  2:315611/140‑1 (MQ=255)                                       cAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgtcagt                                                                                       <  2:871935/140‑1 (MQ=255)                                         ggCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTgtcagtca                                                                                     >  1:957277/1‑140 (MQ=255)                                          gCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGtcat                                                                                    <  2:1232746/140‑1 (MQ=255)                                          gCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGtcat                                                                                    >  1:1048046/1‑140 (MQ=255)                                            ggAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCTCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGtcatca                                                                                  <  2:875566/140‑1 (MQ=255)                                            ggAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGtcatca                                                                                  <  2:1048046/140‑1 (MQ=255)                                            ggAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGtcatca                                                                                  <  2:857199/140‑1 (MQ=255)                                            ggAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGtcatca                                                                                  <  2:957277/140‑1 (MQ=255)                                                   ccGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACctc                                                                           >  1:1189805/1‑140 (MQ=255)                                                      gCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTgt                                                                        <  1:310378/140‑1 (MQ=255)                                                                  aTCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAAtt                                                            >  2:610232/1‑140 (MQ=255)                                                                   tCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAAttt                                                           >  1:610744/1‑140 (MQ=255)                                                                    cACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTa                                                          <  1:915195/140‑1 (MQ=255)                                                                      cTTTCGCTACCCAACCACCTGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATg                                                        <  1:1074053/140‑1 (MQ=255)                                                                          cGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCgg                                                    >  1:1058731/1‑140 (MQ=255)                                                                           gCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGa                                                   <  2:942992/140‑1 (MQ=255)                                                                             tACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACa                                                 >  2:1206163/1‑140 (MQ=255)                                                                                  aaCCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATa                                            <  2:1189805/140‑1 (MQ=255)                                                                                    ccaccGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGc                                          >  1:131644/1‑140 (MQ=255)                                                                                        cGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACtt                                      <  2:131644/140‑1 (MQ=255)                                                                                         ggTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACttt                                     >  1:879968/1‑140 (MQ=255)                                                                                               aGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAg                               <  1:555302/140‑1 (MQ=255)                                                                                                  cTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGaa                            >  1:1252225/1‑140 (MQ=255)                                                                                                  cTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGaa                            <  2:1261837/140‑1 (MQ=255)                                                                                                     ggCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGAt                         >  1:1014899/1‑140 (MQ=255)                                                                                                     ggCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGAt                         >  2:96471/1‑140 (MQ=255)                                                                                                     ggCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGAt                         >  2:1092637/1‑140 (MQ=255)                                                                                                     ggCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGAt                         >  2:616081/1‑140 (MQ=255)                                                                                                          ttgttACGGTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGgtgt                    >  2:244293/1‑140 (MQ=255)                                                                                                                  gTTGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTg            <  2:603135/140‑1 (MQ=255)                                                                                                                    tGCGCCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCa          >  2:705673/1‑140 (MQ=255)                                                                                                                      cgcCACGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAAt        >  1:299285/1‑140 (MQ=255)                                                                                                                           cGGGCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTAc   >  2:1194472/1‑140 (MQ=255)                                                                                                                            gggCTTTCAGCGCCCGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACg  >  2:812867/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                              TGGCTTCGTTACTGTAGGTGACAAATCCGCGTTCAAACCAGGCGGAGCTACCGGCAATATCGGTAATCACTTTCGCTACCCAACCACCGGTACAAGACTCGGCAGTTGTTACGGTTGCGCCACGGGCTTTCAGCGCCTGCCCAACCTGTTCACTTAACTGCATCAGTTCACTGTCAGTCATCACAACCTCTGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACG  >  NC_000913/2824166‑2824428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑