Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	294,733	(T)5→4	coding (67/855 nt)	yagM ←	CP4‑6 prophage; protein YagM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	294,729	0	T	.	100.0%	251.9 / NA	59	coding (71/855 nt)	yagM	CP4‑6 prophage; protein YagM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (35/24);  total (35/24)

CTTCCGCTATTACGGAAGAGTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTGTTTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCC  >  NC_000913/294595‑294863                                                                                                                                       |                                                                                                                                       cTTCCGCTATTACGGAAGAGTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTGttttgc                                                                                                                                   <  1:243810/140‑3 (MQ=255)    ccGCTATTACGGAAGAGTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCtt                                                                                                                               >  2:799592/1‑140 (MQ=255)    ccGCTATTACGGAAGAGTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCtt                                                                                                                               >  1:327851/1‑140 (MQ=255)           tACGGAAGAGTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAgg                                                                                                                        >  2:444242/1‑140 (MQ=255)                    gTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTcc                                                                                                               <  2:765008/140‑1 (MQ=255)                    gTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTcc                                                                                                               >  2:243032/1‑140 (MQ=255)                    gTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTcc                                                                                                               >  1:590699/1‑140 (MQ=255)                     tGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGtttttt                                                                                                                  <  1:168597/136‑1 (MQ=255)                     tGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGtttttt                                                                                                                  >  2:168597/1‑136 (MQ=255)                     tGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCt                                                                                                              >  2:348281/1‑140 (MQ=255)                       agaTTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTgg                                                                                                            >  2:138227/1‑140 (MQ=255)                             atttGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAAc                                                                                                      >  1:348961/1‑140 (MQ=255)                                    aGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTc                                                                                               <  2:914209/140‑1 (MQ=255)                                         ggCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCAtctc                                                                                          >  1:829961/1‑140 (MQ=255)                                                tGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTa                                                                                   >  2:691565/1‑140 (MQ=255)                                                    cAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTaa                                                                               >  1:308444/1‑140 (MQ=255)                                                      aTTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAaca                                                                             <  1:691565/140‑1 (MQ=255)                                                      aTTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAaca                                                                             <  1:412831/140‑1 (MQ=255)                                                        tACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACt                                                                           >  1:690768/1‑140 (MQ=255)                                                         aCTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTa                                                                          >  2:144980/1‑140 (MQ=255)                                                              tAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGa                                                                     <  1:243032/140‑1 (MQ=255)                                                               aaTGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGAc                                                                    >  2:375210/1‑140 (MQ=255)                                                               aaTGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGAc                                                                    >  1:754565/1‑140 (MQ=255)                                                               aaTGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGAc                                                                    >  2:378480/1‑140 (MQ=255)                                                                aTGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACa                                                                   >  1:480333/1‑140 (MQ=255)                                                                     gattgattTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACAttttt                                                              >  1:589379/1‑140 (MQ=255)                                                                     gattgattTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACAttttt                                                              >  1:529469/1‑140 (MQ=255)                                                                          attTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGa                                                         <  2:690768/140‑1 (MQ=255)                                                                          attTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGa                                                         <  2:529469/140‑1 (MQ=255)                                                                          attTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGa                                                         <  2:590699/140‑1 (MQ=255)                                                                          attTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGa                                                         <  1:799592/140‑1 (MQ=255)                                                                           tttttGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGaa                                                        <  1:375210/140‑1 (MQ=255)                                                                               tGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACct                                                    <  2:348961/140‑1 (MQ=255)                                                                                gAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACctc                                                   <  1:165048/140‑1 (MQ=255)                                                                                gAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACctc                                                   <  2:829961/140‑1 (MQ=255)                                                                                 aaCGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACctct                                                  >  1:172314/1‑140 (MQ=255)                                                                                        cTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTTCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTAt                                           <  1:467727/140‑1 (MQ=255)                                                                                        cTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTAt                                           <  2:592793/140‑1 (MQ=255)                                                                                          gAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTAtct                                         <  2:589379/140‑1 (MQ=255)                                                                                          gAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTAtct                                         <  1:444242/140‑1 (MQ=255)                                                                                          gAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTAtct                                         <  1:348281/140‑1 (MQ=255)                                                                                          gAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTAtct                                         <  2:187772/140‑1 (MQ=255)                                                                                            gtgtTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCa                                       <  1:144980/140‑1 (MQ=255)                                                                                             tgtTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCTTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCaa                                      <  1:738894/140‑1 (MQ=255)                                                                                                 tttttATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAAtata                                  <  2:480333/140‑1 (MQ=255)                                                                                                  ttttATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAata                                    >  2:433504/1‑137 (MQ=255)                                                                                                  ttttATGGGAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAata                                    <  1:433504/137‑1 (MQ=255)                                                                                                         ggAGTAGTCTGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAactact                          >  2:570140/1‑140 (MQ=255)                                                                                                                  tGTACTACCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAAt                 >  2:580290/1‑140 (MQ=255)                                                                                                                    tactacCTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAAttt               >  1:687637/1‑140 (MQ=255)                                                                                                                         ccTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAg          >  1:780633/1‑140 (MQ=255)                                                                                                                         ccTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAg          >  1:717591/1‑140 (MQ=255)                                                                                                                         ccTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAg          >  2:694400/1‑140 (MQ=255)                                                                                                                         ccTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAg          >  2:229536/1‑140 (MQ=255)                                                                                                                         ccTGTGACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTCATCTCAATATAAAACTACTATCTGAAATTTCTCAg          >  2:557808/1‑140 (MQ=255)                                                                                                                            gtgACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAgtgt       >  2:611083/1‑140 (MQ=255)                                                                                                                            gtgACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAgtgt       >  2:561106/1‑140 (MQ=255)                                                                                                                              gACTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTg     >  1:329317/1‑140 (MQ=255)                                                                                                                                cTCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGcc   >  1:760577/1‑140 (MQ=255)                                                                                                                                 tCATTG‑TTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGccc  >  2:46039/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       CTTCCGCTATTACGGAAGAGTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTGTTTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCC  >  NC_000913/294595‑294863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑