| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,165,573 | (C)6→5 | intergenic (+52/‑95) | yegP → / → yegQ | DUF1508 domain‑containing protein YegP/putative peptidase YegQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,165,568 | 0 | C | . | 97.1% | 134.2 / ‑2.4 | 34 | intergenic (+47/‑100) | yegP/yegQ | DUF1508 domain‑containing protein YegP/putative peptidase YegQ |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (16/17); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGG > NC_000913/2165434‑2165699 | gATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGccccca < 1:768289/140‑2 (MQ=255)gATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGccccca < 1:577856/140‑2 (MQ=255) aTGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGcccccac > 1:514979/1‑138 (MQ=255) cGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAgcgc > 2:840330/1‑140 (MQ=255) ccGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACaa > 1:816704/1‑140 (MQ=255) ccGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACaa > 1:405495/1‑140 (MQ=255) gcAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAATCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATgcc < 1:534028/140‑1 (MQ=255) cAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATgtc < 1:414148/140‑3 (MQ=255) aCCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTg > 1:117524/1‑140 (MQ=255) ccGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTgg > 2:562876/1‑140 (MQ=255) ccGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTgg > 2:593741/1‑140 (MQ=255) aaTTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGc < 1:636753/140‑1 (MQ=255) aTTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCa < 2:739402/140‑1 (MQ=255) tccctcctTTAAAGCCATGGCCAAAAGCCAGCGGGTGAAAGACAATACGAAATTACGTGGCCGGGCGTGGGTTTTTGGCGTCCGGCGCTTTACCAGA‑CCCCCCACAGCGCTACAAGGCCCGCCCTTAAAGTGGGGGCACt < 2:514979/138‑1 (MQ=255) cGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTa < 2:537022/140‑1 (MQ=255) ttAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAAc < 2:82789/140‑1 (MQ=255) tAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAAcc < 2:117524/140‑1 (MQ=255) tGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTtcatca > 2:526790/1‑140 (MQ=255) gCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAgg < 1:476551/140‑1 (MQ=255) aaGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGaa > 1:898891/1‑140 (MQ=255) aGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAg < 2:279590/140‑1 (MQ=255) cGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCt < 1:562876/140‑1 (MQ=255) gAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGAcc < 1:221773/140‑1 (MQ=255) gACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGtc < 1:840330/140‑1 (MQ=255) tACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAg < 2:816704/140‑1 (MQ=255) tACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAg > 1:240430/1‑140 (MQ=255) aTTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACg > 2:791285/1‑138 (MQ=255) aTTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACg < 1:791285/138‑1 (MQ=255) ttACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACgaga < 1:804152/140‑1 (MQ=255) cGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGaa > 2:915308/1‑140 (MQ=255) gATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAAt < 1:526790/140‑1 (MQ=255) ggCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGttt > 1:186576/1‑140 (MQ=255) gttgttGGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCgg < 2:898891/140‑1 (MQ=255) tgttgGCGTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGaa < 2:405495/140‑1 (MQ=255) gTCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCttt > 2:152226/1‑140 (MQ=255) tCCGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTc > 1:184226/1‑140 (MQ=255) cGGCGCTTTACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTccc > 2:360691/1‑140 (MQ=255) ttACCAGAG‑CCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCggg > 1:720365/1‑140 (MQ=255) | GATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGG > NC_000913/2165434‑2165699 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |