| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,599,887 | (A)5→6 | intergenic (‑229/+42) | livJ ← / ← rpoH | branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein/RNA polymerase, sigma 32 (sigma H) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,599,882 | 1 | . | A | 97.6% | 140.7 / ‑3.4 | 41 | intergenic (‑224/+47) | livJ/rpoH | branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein/RNA polymerase, sigma 32 (sigma H) factor |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (25/15); total (25/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAAC‑AAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAG > NC_000913/3599745‑3600014 | gCTTTGGCAGAATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAaaaaac‑aa > 2:423848/1‑140 (MQ=255)gCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACA‑TTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAaaaaac‑aaa > 1:348604/1‑140 (MQ=255) cTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAaaaaac‑aaa > 2:879707/1‑140 (MQ=255) cTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAaaaaac‑aaa > 1:834052/1‑140 (MQ=255) cTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAaaaaac‑aaa < 1:49469/140‑1 (MQ=255) gcaTACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAAC‑AAAAAaccccg < 2:628775/140‑3 (MQ=255) aCTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGAct < 1:423848/140‑1 (MQ=255) tAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATcc < 2:141338/140‑1 (MQ=255) tAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCAGGACTCTCATcc > 1:240098/1‑140 (MQ=255) aaaaCATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCa < 2:251232/140‑1 (MQ=255) aaaCATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAg > 1:351366/1‑140 (MQ=255) tACCCGATTTTTATGATATTGGAATAGCTATTTTGACA‑TTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTtct < 2:348604/140‑1 (MQ=255) aTTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCt > 2:132149/1‑140 (MQ=255) ttttATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTa < 2:273735/140‑1 (MQ=255) tGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGc < 1:142755/140‑1 (MQ=255) gATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCg < 2:336061/140‑1 (MQ=255) tatTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCAGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGa < 2:240098/140‑1 (MQ=255) ggAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAAtt > 1:230194/1‑140 (MQ=255) gCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCtt > 1:264176/1‑140 (MQ=255) cTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTc > 1:660623/1‑140 (MQ=255) tGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATc < 1:132149/140‑1 (MQ=255) tGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATc > 1:70697/1‑140 (MQ=255) gCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATcg > 1:44698/1‑140 (MQ=255) aTTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCttt > 2:425890/1‑140 (MQ=255) ggCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTcc > 1:314680/1‑140 (MQ=255) cGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAg < 1:425890/140‑1 (MQ=255) ttttGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCt < 1:144725/140‑1 (MQ=255) ttttGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCt < 2:44698/140‑1 (MQ=255) tttGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGtt > 2:652522/1‑128 (MQ=255) tttGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGtt < 1:652522/128‑1 (MQ=255) tttGCCGGAGGGGAAATTGATTATTACAGAGACCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTg > 2:660271/1‑140 (MQ=255) ttGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAg > 1:724480/1‑136 (MQ=255) ttGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAg < 2:724480/136‑1 (MQ=255) ttGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTgg > 1:107079/1‑140 (MQ=255) ttGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTgg > 1:681289/1‑140 (MQ=255) aaTTGATTATTACAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAg > 1:796688/1‑140 (MQ=255) ttattaCAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGaaa > 1:381907/1‑140 (MQ=255) ttattaCAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGaaa > 2:593540/1‑140 (MQ=255) ttattaCAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGaaa > 2:133658/1‑140 (MQ=255) ttattaCAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGaaa > 1:659028/1‑140 (MQ=255) taCAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGcc > 2:713320/1‑140 (MQ=255) cAGAGGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGt > 1:581740/1‑140 (MQ=255) agGCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAAc > 1:712620/1‑140 (MQ=255) gCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACgg > 2:215235/1‑140 (MQ=255) gCCCAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACgg > 1:585177/1‑140 (MQ=255) cccAAAAAACAAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGt < 1:528218/140‑1 (MQ=255) caaaaaacaaaaaaCCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCa > 1:9275/1‑140 (MQ=255) aaaaaacaaaaaacCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAg < 2:264176/140‑1 (MQ=255) aaaaaacaaaaaacCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAg < 2:314680/140‑1 (MQ=255) | GCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAAC‑AAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAG > NC_000913/3599745‑3600014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |