Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,619,496	(A)6→7	coding (377/435 nt)	marR →	DNA‑binding transcriptional repressor MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,619,490	1	.	A	100.0%	166.5 / NA	47	T124K (ACA→AAA)	marR	DNA‑binding transcriptional repressor MarR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (24/23);  total (24/23)

TAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAAC‑AAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACT  >  NC_000913/1619359‑1619622                                                                                                                                     |                                                                                                                                     tAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAAC‑aaaaaaac                                                                                                                              >  2:221169/1‑138 (MQ=255) tAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAAC‑aaaaaaac                                                                                                                              >  1:863298/1‑138 (MQ=255)      gCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGAc                                                                                                                          >  1:14762/1‑140 (MQ=255)      gCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGAc                                                                                                                          >  1:307320/1‑140 (MQ=255)           gTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGAcggcgg                                                                                                                     >  2:526192/1‑140 (MQ=255)               aaaGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGa                                                                                                                 <  2:466028/140‑1 (MQ=255)                aaGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGaa                                                                                                                <  1:210382/140‑1 (MQ=255)                aaGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGaa                                                                                                                <  1:797973/140‑1 (MQ=255)                    ttGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTgg                                                                                                            >  1:563508/1‑140 (MQ=255)                     tGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGc                                                                                                           >  1:624208/1‑140 (MQ=255)                     tGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGc                                                                                                           >  1:545540/1‑140 (MQ=255)                       ccGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCaa                                                                                                         <  2:798340/140‑1 (MQ=255)                        cGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAac                                                                                                        <  1:113606/140‑1 (MQ=255)                            cccGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACtt                                                                                                    <  1:221169/140‑1 (MQ=255)                                aaTGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGt                                                                                                <  2:624208/140‑1 (MQ=255)                                  tGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTAt                                                                                              <  1:358739/140‑1 (MQ=255)                                      aaGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGc                                                                                          >  2:66704/1‑140 (MQ=255)                                           cggcgTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAg                                                                                     >  2:473252/1‑140 (MQ=255)                                              cgTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGaaa                                                                                  >  1:365512/1‑140 (MQ=255)                                                          aCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTaa                                                                      >  2:728383/1‑140 (MQ=255)                                                             tACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACa                                                                   >  2:692916/1‑140 (MQ=255)                                                                caccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGTTTAAGAAAGTCCTGCCGTAAACaaaa                                                                <  1:311576/140‑1 (MQ=255)                                                                caccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACaaaa                                                                <  1:526192/140‑1 (MQ=255)                                                                caccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACaaaa                                                                <  2:563508/140‑1 (MQ=255)                                                                  ccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACaaaaaa                                                              <  2:791580/140‑1 (MQ=255)                                                                  ccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACaaaaaa                                                              >  1:241956/1‑140 (MQ=255)                                                                        gcgcggcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTa                                                        <  1:692916/140‑1 (MQ=255)                                                                             gcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg                                                   <  2:758934/140‑1 (MQ=255)                                                                             gcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg                                                   >  1:196387/1‑140 (MQ=255)                                                                             gcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg                                                   <  2:307320/140‑1 (MQ=255)                                                                                            tGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAAt                                    >  2:818959/1‑140 (MQ=255)                                                                                            tGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAAt                                    >  2:265878/1‑140 (MQ=255)                                                                                            tGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAAt                                    >  2:503119/1‑140 (MQ=255)                                                                                               cATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACt                                 <  1:265878/140‑1 (MQ=255)                                                                                               cATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACt                                 <  2:241956/140‑1 (MQ=255)                                                                                                    aTTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGc                            <  1:884723/140‑1 (MQ=255)                                                                                                         ttGGCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTa                       <  2:837281/140‑1 (MQ=255)                                                                                                           ggCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTAcc                     >  1:639456/1‑140 (MQ=255)                                                                                                           ggCCAGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTAcc                     >  2:882377/1‑140 (MQ=255)                                                                                                               aGGACCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTc                 >  2:173981/1‑140 (MQ=255)                                                                                                                  aCCTGCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATa              <  1:487303/140‑1 (MQ=255)                                                                                                                      gCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCAt          >  1:854724/1‑140 (MQ=255)                                                                                                                      gCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCAt          >  1:76264/1‑140 (MQ=255)                                                                                                                      gCACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCAt          >  1:297125/1‑140 (MQ=255)                                                                                                                       cACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCAtt         >  2:348997/1‑140 (MQ=255)                                                                                                                       cACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCAtt         >  2:203919/1‑140 (MQ=255)                                                                                                                       cACCAAGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCAtt         <  1:473252/140‑1 (MQ=255)                                                                                                                           aaGAATTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTgg     <  2:257099/140‑1 (MQ=255)                                                                                                                              aaTTAACAAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACt  <  2:297125/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     TAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAAC‑AAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATTTTGGACT  >  NC_000913/1619359‑1619622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑