| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,106,518 | (C)6→7 | coding (709/1167 nt) | wbbH ← | putative O‑antigen polymerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,106,512 | 1 | . | C | 95.6% | 156.2 / 0.1 | 45 | coding (715/1167 nt) | wbbH | putative O‑antigen polymerase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (16/27); total (17/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
ACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAT‑‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACA > NC_000913/2106373‑2106645 | aCTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAt < 1:185318/140‑1 (MQ=255)aCTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAt < 1:86560/140‑1 (MQ=255)aCTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAt < 1:756922/140‑1 (MQ=255)aCTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAt < 2:272498/140‑1 (MQ=255)aCTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAt < 2:393556/140‑1 (MQ=255)aCTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAt > 1:604926/1‑140 (MQ=255)aCTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAt < 2:897985/140‑1 (MQ=255)aCTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAt < 1:30895/140‑1 (MQ=255) tCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAT‑‑cc > 1:88699/1‑140 (MQ=255) ccACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAt < 1:437570/137‑1 (MQ=255) ccACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAt > 2:437570/1‑137 (MQ=255) ccACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAT‑‑ccc > 2:860001/1‑140 (MQ=255) ccACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAT‑‑ccc > 1:837844/1‑140 (MQ=255) aTAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAg < 1:296027/140‑1 (MQ=255) aGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTc < 1:568354/140‑1 (MQ=255) ccccATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAGAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTc > 2:754066/1‑138 (MQ=255) ccccATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAGAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTc < 1:754066/138‑1 (MQ=255) cATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGt > 2:496930/1‑140 (MQ=255) cATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGt > 1:807091/1‑140 (MQ=255) cATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGt > 1:810679/1‑140 (MQ=255) tCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCa < 2:88699/140‑1 (MQ=255) tCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCa < 2:196443/140‑1 (MQ=255) ccAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAg < 2:355003/140‑1 (MQ=255) aaGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGgaga < 2:850858/140‑1 (MQ=255) gACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAAt < 1:708888/140‑1 (MQ=255) cATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAg < 1:194577/140‑1 (MQ=255) cTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTa < 1:51852/140‑1 (MQ=255) cTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTa < 2:919935/140‑1 (MQ=255) cTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTa > 2:63125/1‑140 (MQ=255) cTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTa < 2:238953/140‑1 (MQ=255) tGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTa < 2:20797/140‑1 (MQ=255) tGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTAc > 1:697506/1‑140 (MQ=255) tAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATAGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGtaat < 2:807091/140‑1 (MQ=255) tACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGa > 2:205879/1‑140 (MQ=255) ttGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAT‑‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATaaa > 1:510456/1‑140 (MQ=255) gCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAAc > 1:556697/1‑140 (MQ=255) gCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAAc > 1:810793/1‑140 (MQ=255) cTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACa < 2:343836/140‑1 (MQ=255) aaaCTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGtttt < 1:695066/140‑1 (MQ=255) aaCTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTa < 2:708675/140‑1 (MQ=255) cTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTAct < 1:63125/140‑1 (MQ=255) cTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTa > 1:308459/1‑140 (MQ=255) gAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTAtt < 1:619521/140‑1 (MQ=255) gAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTAtt < 1:324421/140‑1 (MQ=255) gggCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGa < 2:810679/140‑1 (MQ=255) ggCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGaa < 2:308459/140‑1 (MQ=255) ggCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGaa > 2:392328/1‑140 (MQ=255) ggCTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGCAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGaa < 2:837844/140‑1 (MQ=255) cTGACCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATg < 1:392328/140‑1 (MQ=255) aCCAAATACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCAt > 1:847556/1‑140 (MQ=255) aaaTACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAag < 1:496930/140‑1 (MQ=255) aaTACATGGATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAaga < 2:714927/140‑1 (MQ=255) aaTACATGGATAGATAATATGCCAT‑‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAagag < 2:510456/140‑1 (MQ=255) ggATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATa > 1:790767/1‑140 (MQ=255) ggATAGATAATATGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATa < 2:604926/140‑1 (MQ=255) ataataTGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATa > 2:632118/1‑140 (MQ=255) atGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGAc > 1:739145/1‑140 (MQ=255) tGCCATC‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACa > 2:373084/1‑140 (MQ=255) | ACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGATTATAGGGCTGACCAAATACATGGATAGATAATATGCCAT‑‑CCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACA > NC_000913/2106373‑2106645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |